| Summary: | Anaerobic digestion (AD) of chicken litter (CL) is a viable alternative to disposal. However, methane yields from this primarily organic waste are quite low when mono-digested. This paper discusses the effect of an enzyme cocktail, trace element (TE) supplementation and selenium (Se) addition in small-scale batch biomethane potential (BMP) assays to enhance the AD of CL. Eleven different assays were set up in triplicate including assays containing only inoculum (blank), only CL (negative control) and cellulose and inoculum (positive control). The results indicate that both enzyme treatment and trace element supplementation enhanced the biogas and methane yield. The highest specific biogas and methane yields were noted for 1% enzyme-treated CL of 835.2 L/kg volatile solids (VS) and 460.8 L/kg VS, respectively. Usually, mono-digestion of CL is low due to high nitrogen content and the presence of recalcitrant lignocellulosic material from the bedding material. Enzyme treatment performed better than the addition of the TE mix and Se.
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