Expression, activity measurement and structure prediction of spermatogenic regulator:recombinant human HSF5

Objective To obtain soluble expression of recombinant human heat shock transcription factor 5(rhHSF5) proteins and predict the structure of HSF5 bound to heat shock element. Methods The nucleotide sequence encoding the target gene was subcloned into expression vector pET-22b(+), followed by t...

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Bibliographic Details
Main Author: ZHONG Hong-li, XIAO Cheng-liang, SHI Xiang-rui, DAI Yu-jie, LIU Wei, ZHANG Qing-hua
Format: Article
Language:zho
Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2021-09-01
Series:Jichu yixue yu linchuang
Subjects:
Online Access:http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210172.pdf