<i>Trichoderma</i> spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae
<i>Pinus sylvestris</i> bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of <i>P. sylvestris</i> bark against <i>Botryosphaeria dothidea</i>,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/12/12/1731 |
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| author | Vera Karličić Milica Zlatković Jelena Jovičić-Petrović Milan P. Nikolić Saša Orlović Vera Raičević |
| author_facet | Vera Karličić Milica Zlatković Jelena Jovičić-Petrović Milan P. Nikolić Saša Orlović Vera Raičević |
| author_sort | Vera Karličić |
| collection | DOAJ |
| description | <i>Pinus sylvestris</i> bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of <i>P. sylvestris</i> bark against <i>Botryosphaeria dothidea</i>, <i>Dothiorella sarmentorum</i>, and <i>Neofusicoccum parvum</i> (Botryosphaeriaceae) through its chemical (water extracts) and biological (<i>Trichoderma</i> spp. isolated from the bark) components. The water bark extracts were prepared at two temperatures (80 and 120 °C) and pH regimes (7 and 9). The presence of bark extracts (30%) caused inhibition of mycelial growth of <i>B. dothidea</i> and <i>D. sarmentorum</i> for 39 to 44% and 53 to 60%, respectively. Moreover, we studied the antagonistic effect of three <i>Trichoderma</i> isolates originating from the pine bark. <i>Trichoderma</i> spp. reduced growth of <i>B. dothidea</i> by 67%–85%, <i>D. sarmentorum</i> by 63%–75% and <i>N. parvum</i> by 55%–62%. Microscopic examination confirmed typical mycoparasitism manifestations (coiling, parallel growth, hook-like structures). The isolates produced cellulase, β-glucosidase and N-acetyl-β-glucosaminidase. The volatile blend detected the emission of several volatile compounds with antimicrobial activity, including nonanoic acid, cubenene, cis-α-bergamotene, hexanedioic acid, and verticillol. The present study confirmed in vitro potential of <i>P. sylvestris</i> bark extracts and <i>Trichoderma</i> spp. against the Botryosphaeriaceae. The study is an important step towards the use of environmentally friendly methods of Botryosphaeriaceae disease control. |
| first_indexed | 2024-03-10T04:05:53Z |
| format | Article |
| id | doaj.art-09933789861545119eef33e1ccac6029 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T04:05:53Z |
| publishDate | 2021-12-01 |
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| series | Forests |
| spelling | doaj.art-09933789861545119eef33e1ccac60292023-11-23T08:21:37ZengMDPI AGForests1999-49072021-12-011212173110.3390/f12121731<i>Trichoderma</i> spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against BotryosphaeriaceaeVera Karličić0Milica Zlatković1Jelena Jovičić-Petrović2Milan P. Nikolić3Saša Orlović4Vera Raičević5Faculty of Agriculture, University of Belgrade, 11000 Belgrade, SerbiaInstitute of Lowland Forestry and Environment (ILFE), University of Novi Sad, 21102 Novi Sad, SerbiaFaculty of Agriculture, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Agronomy, University of Kragujevac, 32000 Čačak, SerbiaInstitute of Lowland Forestry and Environment (ILFE), University of Novi Sad, 21102 Novi Sad, SerbiaFaculty of Agriculture, University of Belgrade, 11000 Belgrade, Serbia<i>Pinus sylvestris</i> bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of <i>P. sylvestris</i> bark against <i>Botryosphaeria dothidea</i>, <i>Dothiorella sarmentorum</i>, and <i>Neofusicoccum parvum</i> (Botryosphaeriaceae) through its chemical (water extracts) and biological (<i>Trichoderma</i> spp. isolated from the bark) components. The water bark extracts were prepared at two temperatures (80 and 120 °C) and pH regimes (7 and 9). The presence of bark extracts (30%) caused inhibition of mycelial growth of <i>B. dothidea</i> and <i>D. sarmentorum</i> for 39 to 44% and 53 to 60%, respectively. Moreover, we studied the antagonistic effect of three <i>Trichoderma</i> isolates originating from the pine bark. <i>Trichoderma</i> spp. reduced growth of <i>B. dothidea</i> by 67%–85%, <i>D. sarmentorum</i> by 63%–75% and <i>N. parvum</i> by 55%–62%. Microscopic examination confirmed typical mycoparasitism manifestations (coiling, parallel growth, hook-like structures). The isolates produced cellulase, β-glucosidase and N-acetyl-β-glucosaminidase. The volatile blend detected the emission of several volatile compounds with antimicrobial activity, including nonanoic acid, cubenene, cis-α-bergamotene, hexanedioic acid, and verticillol. The present study confirmed in vitro potential of <i>P. sylvestris</i> bark extracts and <i>Trichoderma</i> spp. against the Botryosphaeriaceae. The study is an important step towards the use of environmentally friendly methods of Botryosphaeriaceae disease control.https://www.mdpi.com/1999-4907/12/12/1731Botryosphaeriaceaebiocontrolpine bark extracts<i>Trichoderma citrinoviride</i>VOCslytic enzymes |
| spellingShingle | Vera Karličić Milica Zlatković Jelena Jovičić-Petrović Milan P. Nikolić Saša Orlović Vera Raičević <i>Trichoderma</i> spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae Forests Botryosphaeriaceae biocontrol pine bark extracts <i>Trichoderma citrinoviride</i> VOCs lytic enzymes |
| title | <i>Trichoderma</i> spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae |
| title_full | <i>Trichoderma</i> spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae |
| title_fullStr | <i>Trichoderma</i> spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae |
| title_full_unstemmed | <i>Trichoderma</i> spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae |
| title_short | <i>Trichoderma</i> spp. from Pine Bark and Pine Bark Extracts: Potent Biocontrol Agents against Botryosphaeriaceae |
| title_sort | i trichoderma i spp from pine bark and pine bark extracts potent biocontrol agents against botryosphaeriaceae |
| topic | Botryosphaeriaceae biocontrol pine bark extracts <i>Trichoderma citrinoviride</i> VOCs lytic enzymes |
| url | https://www.mdpi.com/1999-4907/12/12/1731 |
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