CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESIS
Chiral separation cetirizine, a second generation H1 antagonist was studied by cyclodextrine (CD) mediated capillary electrophoresis. The influence on the separation of several parameters including pH and concentration of the background electrolyte (BGE), CD type and concentration, applied voltage a...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2014-10-01
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| Series: | Indonesian Journal of Pharmacy |
| Subjects: | |
| Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/271 |
| _version_ | 1818041540407197696 |
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| author | Gabriel Hancu Monica Budău Lajos Attila Papp Aura Rusu Eleonora Mircia |
| author_facet | Gabriel Hancu Monica Budău Lajos Attila Papp Aura Rusu Eleonora Mircia |
| author_sort | Gabriel Hancu |
| collection | DOAJ |
| description | Chiral separation cetirizine, a second generation H1 antagonist was studied by cyclodextrine (CD) mediated capillary electrophoresis. The influence on the separation of several parameters including pH and concentration of the background electrolyte (BGE), CD type and concentration, applied voltage and temperature were studied and the electrophoretic and analytic parameters were optimized. The best conditions for the chiral separation were obtained using 25mM disodium hydrogeno-phosphate – 25mM sodium didydrogeno-phosphate (1:1) as BGE, 5mM sulfobuthyl ether- β-CD as chiral selector, a voltage of + 20kV, temperature of 20°C, injection pressure/time of 50mbar/ 1sec, UV detection at 230nm. The analytical performance of the method was evaluated. The proposed method was successfully applied to the enantioselective assay of cetirizine in pharmaceutical formula-tions. CE proved to be a rapid, specific, reliable and cost-effective method for the chiral separation of cetirizine enantiomers and can be useful for laboratories performing routine analysis. |
| first_indexed | 2024-12-10T08:32:03Z |
| format | Article |
| id | doaj.art-0997ed62e34340f19c7e90786a151342 |
| institution | Directory Open Access Journal |
| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-12-10T08:32:03Z |
| publishDate | 2014-10-01 |
| publisher | Universitas Gadjah Mada |
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| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-0997ed62e34340f19c7e90786a1513422022-12-22T01:56:03ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862014-10-0125422322910.14499/indonesianjpharm25iss4pp223CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESISGabriel Hancu0Monica Budău1Lajos Attila Papp2Aura Rusu3 Eleonora Mircia4Dept of Pharmaceutical Chemistry, Faculty of Pharmacy, Univ. of Medicine and Pharmacy, Tîrgu Mureş, Romania; Gh. Marinescu 38, 540000Dept of Pharmaceutical Chemistry, Faculty of Pharmacy, Univ. of Medicine and Pharmacy, Tîrgu Mureş, Romania; Gh. Marinescu 38, 540000Dept of Pharmaceutical Chemistry, Faculty of Pharmacy, Univ. of Medicine and Pharmacy, Tîrgu Mureş, Romania; Gh. Marinescu 38, 540000Dept of Pharmaceutical Chemistry, Faculty of Pharmacy, Univ. of Medicine and Pharmacy, Tîrgu Mureş, Romania; Gh. Marinescu 38, 540000Dept of Organic Chemistry, Faculty of Pharmacy, Univ. of Medicine and Pharmacy, Tîrgu Mureş, Romania; Gh. Marinescu 38, 540000Chiral separation cetirizine, a second generation H1 antagonist was studied by cyclodextrine (CD) mediated capillary electrophoresis. The influence on the separation of several parameters including pH and concentration of the background electrolyte (BGE), CD type and concentration, applied voltage and temperature were studied and the electrophoretic and analytic parameters were optimized. The best conditions for the chiral separation were obtained using 25mM disodium hydrogeno-phosphate – 25mM sodium didydrogeno-phosphate (1:1) as BGE, 5mM sulfobuthyl ether- β-CD as chiral selector, a voltage of + 20kV, temperature of 20°C, injection pressure/time of 50mbar/ 1sec, UV detection at 230nm. The analytical performance of the method was evaluated. The proposed method was successfully applied to the enantioselective assay of cetirizine in pharmaceutical formula-tions. CE proved to be a rapid, specific, reliable and cost-effective method for the chiral separation of cetirizine enantiomers and can be useful for laboratories performing routine analysis.http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/271chiral separationcetirizinecapillary electrophoresis |
| spellingShingle | Gabriel Hancu Monica Budău Lajos Attila Papp Aura Rusu Eleonora Mircia CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESIS Indonesian Journal of Pharmacy chiral separation cetirizine capillary electrophoresis |
| title | CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESIS |
| title_full | CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESIS |
| title_fullStr | CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESIS |
| title_full_unstemmed | CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESIS |
| title_short | CHIRAL SEPARATION OF CETIRIZINE ENANTIOMERS BY CYCLODEXTRIN MEDIATED CAPILLARY ELECTROPHORESIS |
| title_sort | chiral separation of cetirizine enantiomers by cyclodextrin mediated capillary electrophoresis |
| topic | chiral separation cetirizine capillary electrophoresis |
| url | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/271 |
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