Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips
Organ-on-chip (OoC) devices are increasingly used to mimic the tissue microenvironment of cells in intact organs. This includes microchannels to mimic, for example, fluidic flow through blood vessels. Present methods for controlling microfluidic flow in these systems rely on gravity, rocker systems...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/8/1359 |
| _version_ | 1827617688780800000 |
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| author | Mees N. S. de Graaf Aisen Vivas Andries D. van der Meer Christine L. Mummery Valeria V. Orlova |
| author_facet | Mees N. S. de Graaf Aisen Vivas Andries D. van der Meer Christine L. Mummery Valeria V. Orlova |
| author_sort | Mees N. S. de Graaf |
| collection | DOAJ |
| description | Organ-on-chip (OoC) devices are increasingly used to mimic the tissue microenvironment of cells in intact organs. This includes microchannels to mimic, for example, fluidic flow through blood vessels. Present methods for controlling microfluidic flow in these systems rely on gravity, rocker systems or external pressure pumps. For many purposes, pressure pumps give the most consistent flow profiles, but they are not well-suited for high throughput as might be required for testing drug responses. Here, we describe a method which allows for multiplexing of microfluidic channels in OoC devices plus the accompanying custom software necessary to run the system. Moreover, we show the approach is also suitable for recirculation of culture medium, an essential cost consideration when expensive culture reagents are used and are not “spent” through uptake by the cells during transient unidirectional flow. |
| first_indexed | 2024-03-09T09:51:33Z |
| format | Article |
| id | doaj.art-0998b380767740f0bdcac4f5f3ff011e |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T09:51:33Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-0998b380767740f0bdcac4f5f3ff011e2023-12-02T00:02:06ZengMDPI AGMicromachines2072-666X2022-08-01138135910.3390/mi13081359Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-ChipsMees N. S. de Graaf0Aisen Vivas1Andries D. van der Meer2Christine L. Mummery3Valeria V. Orlova4Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The NetherlandsApplied Stem Cell Technologies, Technical Medical Centre, University of Twente, 7500 AE Enschede, The NetherlandsApplied Stem Cell Technologies, Technical Medical Centre, University of Twente, 7500 AE Enschede, The NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The NetherlandsDepartment of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The NetherlandsOrgan-on-chip (OoC) devices are increasingly used to mimic the tissue microenvironment of cells in intact organs. This includes microchannels to mimic, for example, fluidic flow through blood vessels. Present methods for controlling microfluidic flow in these systems rely on gravity, rocker systems or external pressure pumps. For many purposes, pressure pumps give the most consistent flow profiles, but they are not well-suited for high throughput as might be required for testing drug responses. Here, we describe a method which allows for multiplexing of microfluidic channels in OoC devices plus the accompanying custom software necessary to run the system. Moreover, we show the approach is also suitable for recirculation of culture medium, an essential cost consideration when expensive culture reagents are used and are not “spent” through uptake by the cells during transient unidirectional flow.https://www.mdpi.com/2072-666X/13/8/1359organ-on-a-chip (OoC)vessels-on-chip (VoC)fluidic circuit board (FCB)multiplexingperfusionrecirculation |
| spellingShingle | Mees N. S. de Graaf Aisen Vivas Andries D. van der Meer Christine L. Mummery Valeria V. Orlova Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips Micromachines organ-on-a-chip (OoC) vessels-on-chip (VoC) fluidic circuit board (FCB) multiplexing perfusion recirculation |
| title | Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips |
| title_full | Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips |
| title_fullStr | Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips |
| title_full_unstemmed | Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips |
| title_short | Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips |
| title_sort | pressure driven perfusion system to control multiplex and recirculate cell culture medium for organs on chips |
| topic | organ-on-a-chip (OoC) vessels-on-chip (VoC) fluidic circuit board (FCB) multiplexing perfusion recirculation |
| url | https://www.mdpi.com/2072-666X/13/8/1359 |
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