ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3′-hydroxylase gene in Zinnia elegans
Petal color in Zinnia elegans is characterized mainly by anthocyanin accumulation. The difference in the content of anthocyanins, especially cyanidins, affects petal coloration in Z. elegans, but the underlying regulatory mechanism remains elusive. Here, we report one R2R3-MYB transcription factor f...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.981086/full |
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| author | Jieyu Qian Jieyu Qian Jieyu Qian Lingli Jiang Lingli Jiang Lingli Jiang Hongsheng Qing Hongsheng Qing Hongsheng Qing Jiahong Chen Jiahong Chen Jiahong Chen Ziyun Wan Ziyun Wan Ziyun Wan Menghan Xu Menghan Xu Menghan Xu Jianxin Fu Jianxin Fu Jianxin Fu Chao Zhang Chao Zhang Chao Zhang |
| author_facet | Jieyu Qian Jieyu Qian Jieyu Qian Lingli Jiang Lingli Jiang Lingli Jiang Hongsheng Qing Hongsheng Qing Hongsheng Qing Jiahong Chen Jiahong Chen Jiahong Chen Ziyun Wan Ziyun Wan Ziyun Wan Menghan Xu Menghan Xu Menghan Xu Jianxin Fu Jianxin Fu Jianxin Fu Chao Zhang Chao Zhang Chao Zhang |
| author_sort | Jieyu Qian |
| collection | DOAJ |
| description | Petal color in Zinnia elegans is characterized mainly by anthocyanin accumulation. The difference in the content of anthocyanins, especially cyanidins, affects petal coloration in Z. elegans, but the underlying regulatory mechanism remains elusive. Here, we report one R2R3-MYB transcription factor from subgroup 6, ZeMYB9, acting as a positive regulator of anthocyanin accumulation in Z. elegans. Up-regulated expression of ZeMYB9 and flavonoid 3′-hydroxylase gene (ZeF3’H) was detected in the cultivar with higher cyanidin content. ZeMYB9 could specifically activate the promoter of ZeF3’H, and over-expression of ZeMYB9 induces much greater anthocyanin accumulation and higher expression level of anthocyanin biosynthetic genes in both petunia and tobacco. And then, ZeMYB9 was demonstrated to interact with ZeGL3, a bHLH transcription factor belonging to IIIf subgroup. Promoter activity of ZeF3’H was significantly promoted by co-expressing ZeMYB9 and ZeGL3 compared with expressing ZeMYB9 alone. Moreover, transient co-expression of ZeMYB9 and ZeGL3 induced anthocyanin accumulation in tobacco leaves. Our results suggest that ZeMYB9 could enhance cyanidin synthesis and regulate petal color in Z. elegans though activating the expression of ZeF3’H, by itself or interacting with ZeGL3. |
| first_indexed | 2024-04-11T09:27:32Z |
| format | Article |
| id | doaj.art-0999db92803f446fa59f1f48212c1ee8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T09:27:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-0999db92803f446fa59f1f48212c1ee82022-12-22T04:31:59ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.981086981086ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3′-hydroxylase gene in Zinnia elegansJieyu Qian0Jieyu Qian1Jieyu Qian2Lingli Jiang3Lingli Jiang4Lingli Jiang5Hongsheng Qing6Hongsheng Qing7Hongsheng Qing8Jiahong Chen9Jiahong Chen10Jiahong Chen11Ziyun Wan12Ziyun Wan13Ziyun Wan14Menghan Xu15Menghan Xu16Menghan Xu17Jianxin Fu18Jianxin Fu19Jianxin Fu20Chao Zhang21Chao Zhang22Chao Zhang23Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaKey Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaSchool of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaKey Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaSchool of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaKey Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaSchool of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaKey Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaSchool of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaKey Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaSchool of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaKey Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaSchool of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaKey Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaSchool of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaZhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaKey Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaSchool of Landscape Architecture, Zhejiang Agriculture & Forestry University, Hangzhou, ChinaPetal color in Zinnia elegans is characterized mainly by anthocyanin accumulation. The difference in the content of anthocyanins, especially cyanidins, affects petal coloration in Z. elegans, but the underlying regulatory mechanism remains elusive. Here, we report one R2R3-MYB transcription factor from subgroup 6, ZeMYB9, acting as a positive regulator of anthocyanin accumulation in Z. elegans. Up-regulated expression of ZeMYB9 and flavonoid 3′-hydroxylase gene (ZeF3’H) was detected in the cultivar with higher cyanidin content. ZeMYB9 could specifically activate the promoter of ZeF3’H, and over-expression of ZeMYB9 induces much greater anthocyanin accumulation and higher expression level of anthocyanin biosynthetic genes in both petunia and tobacco. And then, ZeMYB9 was demonstrated to interact with ZeGL3, a bHLH transcription factor belonging to IIIf subgroup. Promoter activity of ZeF3’H was significantly promoted by co-expressing ZeMYB9 and ZeGL3 compared with expressing ZeMYB9 alone. Moreover, transient co-expression of ZeMYB9 and ZeGL3 induced anthocyanin accumulation in tobacco leaves. Our results suggest that ZeMYB9 could enhance cyanidin synthesis and regulate petal color in Z. elegans though activating the expression of ZeF3’H, by itself or interacting with ZeGL3.https://www.frontiersin.org/articles/10.3389/fpls.2022.981086/fullZinnia elegansanthocyaninflavonoid 3′-hydroxylaseMYBbHLH |
| spellingShingle | Jieyu Qian Jieyu Qian Jieyu Qian Lingli Jiang Lingli Jiang Lingli Jiang Hongsheng Qing Hongsheng Qing Hongsheng Qing Jiahong Chen Jiahong Chen Jiahong Chen Ziyun Wan Ziyun Wan Ziyun Wan Menghan Xu Menghan Xu Menghan Xu Jianxin Fu Jianxin Fu Jianxin Fu Chao Zhang Chao Zhang Chao Zhang ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3′-hydroxylase gene in Zinnia elegans Frontiers in Plant Science Zinnia elegans anthocyanin flavonoid 3′-hydroxylase MYB bHLH |
| title | ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3′-hydroxylase gene in Zinnia elegans |
| title_full | ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3′-hydroxylase gene in Zinnia elegans |
| title_fullStr | ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3′-hydroxylase gene in Zinnia elegans |
| title_full_unstemmed | ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3′-hydroxylase gene in Zinnia elegans |
| title_short | ZeMYB9 regulates cyanidin synthesis by activating the expression of flavonoid 3′-hydroxylase gene in Zinnia elegans |
| title_sort | zemyb9 regulates cyanidin synthesis by activating the expression of flavonoid 3 hydroxylase gene in zinnia elegans |
| topic | Zinnia elegans anthocyanin flavonoid 3′-hydroxylase MYB bHLH |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.981086/full |
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