Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study
IntroductionAnti-glomerular basement membrane (GBM) antibodies are pathogenic antibodies first detected in renal-limited anti-GBM disease and in Goodpasture disease, the latter characterized by rapidly progressive crescentic glomerulonephritis combined with intra-alveolar hemorrhage. Studies have su...
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Frontiers Media S.A.
2020-12-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.597863/full |
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| author | Nellie Bourse Chalvon Pauline Orquevaux Delphine Giusti Gregory Gatouillat Thierry Tabary Marcelle Tonye Libyh Jan Chrusciel Moustapha Drame Grace Stockton-Bliard Zahir Amoura Laurent Arnaud Hanns-Martin Lorenz Gilles Blaison Bernard Bonnotte Nadine Magy-Bertrand Sabine Revuz Reinhard Edmund Voll Oliver Hinschberger Andreas Schwarting Bach Nga Pham Thierry Martin Jean-Loup Pennaforte Amelie Servettaz |
| author_facet | Nellie Bourse Chalvon Pauline Orquevaux Delphine Giusti Gregory Gatouillat Thierry Tabary Marcelle Tonye Libyh Jan Chrusciel Moustapha Drame Grace Stockton-Bliard Zahir Amoura Laurent Arnaud Hanns-Martin Lorenz Gilles Blaison Bernard Bonnotte Nadine Magy-Bertrand Sabine Revuz Reinhard Edmund Voll Oliver Hinschberger Andreas Schwarting Bach Nga Pham Thierry Martin Jean-Loup Pennaforte Amelie Servettaz |
| author_sort | Nellie Bourse Chalvon |
| collection | DOAJ |
| description | IntroductionAnti-glomerular basement membrane (GBM) antibodies are pathogenic antibodies first detected in renal-limited anti-GBM disease and in Goodpasture disease, the latter characterized by rapidly progressive crescentic glomerulonephritis combined with intra-alveolar hemorrhage. Studies have suggested that anti-GBM antibody positivity may be of interest in lupus nephritis (LN). Moreover, severe anti-GBM vasculitis cases in patients with systemic lupus erythematosus (SLE) have been described in the literature, but few studies have assessed the incidence of anti-GBM antibodies in SLE patients.ObjectiveThe main study objective was to determine if positive anti-GBM antibodies were present in the serum of SLE patients with or without proliferative renal damage and compared to a healthy control group.MethodologyThis retrospective study was performed on SLE patients’ sera from a Franco-German European biobank, developed between 2011 and 2014, from 17 hospital centers in the Haut-Rhin region. Patients were selected according to their renal involvement, and matched by age and gender. The serum from healthy voluntary blood donors was also tested. Anti-GBM were screened by fluorescence enzyme immunoassay (FEIA), and then by indirect immunofluorescence (IIF) in case of low reactivity detection (titer >6 U/ml).ResultsThe cohort was composed of 100 SLE patients with proliferative LN (27% with class III, 67% with class IV, and 6% with class V), compared to 100 SLE patients without LN and 100 controls. Patients were mostly Caucasian and met the ACR 1997 criteria and/or the SLICC 2012 criteria. Among the 300 tested sera, no significant levels of anti-GBM antibodies were detected (>10 U/ml) by the automated technique, three sera were found “ambivalent” (>7 U/ml): one in the SLE with LN group and two in the SLE without LN group. Subsequent IIF assays did not detect anti-GBM antibodies.ConclusionAnti-GBM antibodies were not detected in the serum of Caucasian patients with SLE, even in case of renal involvement, a situation favoring the antigenic exposure of glomerular basement membranes. Our results reaffirm the central role of anti-GBM antibodies as a specific diagnostic biomarker for Goodpasture vasculitis and therefore confirm that anti-GBM antibody must not be carried out in patients with SLE (with or without LN) in the absence of disease-suggestive symptoms. |
| first_indexed | 2024-12-21T14:34:52Z |
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| publishDate | 2020-12-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-099b092abb9544c0b34f557d13543e1d2022-12-21T19:00:22ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-12-011110.3389/fimmu.2020.597863597863Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS StudyNellie Bourse Chalvon0Pauline Orquevaux1Delphine Giusti2Gregory Gatouillat3Thierry Tabary4Marcelle Tonye Libyh5Jan Chrusciel6Moustapha Drame7Grace Stockton-Bliard8Zahir Amoura9Laurent Arnaud10Hanns-Martin Lorenz11Gilles Blaison12Bernard Bonnotte13Nadine Magy-Bertrand14Sabine Revuz15Reinhard Edmund Voll16Oliver Hinschberger17Andreas Schwarting18Bach Nga Pham19Thierry Martin20Jean-Loup Pennaforte21Amelie Servettaz22Département de médecine interne, Centre Hospitalier Universitaire de Reims, Reims, FranceDépartement de médecine interne, Centre Hospitalier Universitaire de Reims, Reims, FranceDépartement d'immunologie biologique (laboratoire d'immunologie), Centre Hospitalier Universitaire de Reims, Reims, FranceDépartement d'immunologie biologique (laboratoire d'immunologie), Centre Hospitalier Universitaire de Reims, Reims, FranceDépartement d'immunologie biologique (laboratoire d'immunologie), Centre Hospitalier Universitaire de Reims, Reims, FranceDépartement d'immunologie biologique (laboratoire d'immunologie), Centre Hospitalier Universitaire de Reims, Reims, FranceDépartement d'information médicale et d'évaluation des performances, santé publique, Centre Hospitalier de Troyes, Troyes, FranceDépartement de Délégation à la Recherche Clinique et à l'Innovation, University Hospital of Martinique, Fort-de-France, MartiniqueCommission de la recherche, Université de Reims Champagne-Ardenne, Reims, FranceService de Médecine interne, Assistance Publique Hôpitaux de Paris (APHP), Paris, FranceService de Rhumatologie, Hôpitaux Universitaires de Strasbourg, Strasbourg, FranceDivision of Rheumatology, Clinic for Hematology, Oncology and Rheumatology, Department of Internal Medicine V, University Hospital Heidelberg, Heidelberg, GermanyDépartement de médecine interne, Hôpital Pasteur, Colmar, France0Département de Médecine Interne et d’immunologie Clinique, Centre Hospitalier Regional Universitaire De Dijon, Dijon, France1Département de médecine interne, Centre Hospitalier Universitaire de Besançon, Besancon, France2Département de médecine interne, Hôpital Belle-Isle, Metz, France3Department of Rheumatology and Clinical Immunology, Medical Center, University of Freiburg, Freiburg, Germany4Département de médecine interne, Groupe Hospitalier de la Région de Mulhouse et Sud Alsace (GHRMSA), Mulhouse, France5Universitäres Centrum für Autoimmunität Mainz, Johannes Gutenberg-Universität Mainz, Mainz, GermanyDépartement d'immunologie biologique (laboratoire d'immunologie), Centre Hospitalier Universitaire de Reims, Reims, France6Immunologie Clinique et Médecine Interne, Hôpitaux Universitaires de Strasbourg, Strasbourg, FranceDépartement de médecine interne, Centre Hospitalier Universitaire de Reims, Reims, FranceDépartement de médecine interne, Centre Hospitalier Universitaire de Reims, Reims, FranceIntroductionAnti-glomerular basement membrane (GBM) antibodies are pathogenic antibodies first detected in renal-limited anti-GBM disease and in Goodpasture disease, the latter characterized by rapidly progressive crescentic glomerulonephritis combined with intra-alveolar hemorrhage. Studies have suggested that anti-GBM antibody positivity may be of interest in lupus nephritis (LN). Moreover, severe anti-GBM vasculitis cases in patients with systemic lupus erythematosus (SLE) have been described in the literature, but few studies have assessed the incidence of anti-GBM antibodies in SLE patients.ObjectiveThe main study objective was to determine if positive anti-GBM antibodies were present in the serum of SLE patients with or without proliferative renal damage and compared to a healthy control group.MethodologyThis retrospective study was performed on SLE patients’ sera from a Franco-German European biobank, developed between 2011 and 2014, from 17 hospital centers in the Haut-Rhin region. Patients were selected according to their renal involvement, and matched by age and gender. The serum from healthy voluntary blood donors was also tested. Anti-GBM were screened by fluorescence enzyme immunoassay (FEIA), and then by indirect immunofluorescence (IIF) in case of low reactivity detection (titer >6 U/ml).ResultsThe cohort was composed of 100 SLE patients with proliferative LN (27% with class III, 67% with class IV, and 6% with class V), compared to 100 SLE patients without LN and 100 controls. Patients were mostly Caucasian and met the ACR 1997 criteria and/or the SLICC 2012 criteria. Among the 300 tested sera, no significant levels of anti-GBM antibodies were detected (>10 U/ml) by the automated technique, three sera were found “ambivalent” (>7 U/ml): one in the SLE with LN group and two in the SLE without LN group. Subsequent IIF assays did not detect anti-GBM antibodies.ConclusionAnti-GBM antibodies were not detected in the serum of Caucasian patients with SLE, even in case of renal involvement, a situation favoring the antigenic exposure of glomerular basement membranes. Our results reaffirm the central role of anti-GBM antibodies as a specific diagnostic biomarker for Goodpasture vasculitis and therefore confirm that anti-GBM antibody must not be carried out in patients with SLE (with or without LN) in the absence of disease-suggestive symptoms.https://www.frontiersin.org/articles/10.3389/fimmu.2020.597863/fullanti-glomerular basement membrane antibodieslupus nephritisGoodpasture diseaseanti-GBM antibodiesanti-GBM glomerulonephritissystemic lupus erythematosus |
| spellingShingle | Nellie Bourse Chalvon Pauline Orquevaux Delphine Giusti Gregory Gatouillat Thierry Tabary Marcelle Tonye Libyh Jan Chrusciel Moustapha Drame Grace Stockton-Bliard Zahir Amoura Laurent Arnaud Hanns-Martin Lorenz Gilles Blaison Bernard Bonnotte Nadine Magy-Bertrand Sabine Revuz Reinhard Edmund Voll Oliver Hinschberger Andreas Schwarting Bach Nga Pham Thierry Martin Jean-Loup Pennaforte Amelie Servettaz Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study Frontiers in Immunology anti-glomerular basement membrane antibodies lupus nephritis Goodpasture disease anti-GBM antibodies anti-GBM glomerulonephritis systemic lupus erythematosus |
| title | Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study |
| title_full | Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study |
| title_fullStr | Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study |
| title_full_unstemmed | Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study |
| title_short | Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of the GOODLUPUS Study |
| title_sort | absence of anti glomerular basement membrane antibodies in 200 patients with systemic lupus erythematosus with or without lupus nephritis results of the goodlupus study |
| topic | anti-glomerular basement membrane antibodies lupus nephritis Goodpasture disease anti-GBM antibodies anti-GBM glomerulonephritis systemic lupus erythematosus |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.597863/full |
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