Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangrove
Five Schizochytrium strains (N-1, N-2, N-5, N-6, and N-9) were isolated from fallen, senescent leaves of mangrove tree (Kandelia candel) in Hong Kong. The fungi were cultivated in glucose yeast extract medium containing 60 g of glucose, 10 g of yeast extract and 1 L of 15‰ artificial seawater, initi...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2003-09-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/25-5-pdf/11fatty-acid.pdf |
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| author | K.W. Fan Niyom Kamlangdee |
| author_facet | K.W. Fan Niyom Kamlangdee |
| author_sort | K.W. Fan |
| collection | DOAJ |
| description | Five Schizochytrium strains (N-1, N-2, N-5, N-6, and N-9) were isolated from fallen, senescent leaves of mangrove tree (Kandelia candel) in Hong Kong. The fungi were cultivated in glucose yeast extract medium containing 60 g of glucose, 10 g of yeast extract and 1 L of 15‰ artificial seawater, initial pH 6.0, with shaking for 52 hr at 25ºC. Biomass yields of 5 isolates ranged from 10.8 to 13.2 g/l. Isolate N-2 yielding the highest dried cell mass at 13.2 g/l and isolate N-9 grew poorly with 10.8 g/l of biomass. EPA (Eicosapentaenoic acid, 20:5n-3) yield was low in most strains, while DHA (Docosahexaenoic acid, 22:6n-3) was high on the same medium. The contents of DHA in biomass varied: 174.9, 203.6, 186.1, 171.3 and 157.9 mg/g of dried-biomass for Schizochytrium isolate N-1, N-2, N-5, N-6, and N-9, respectively. Isolate N-2 had the highest proportion of DHA in fatty acid profile with 15:0, 28.7%; 16:0, 21.3%; 18:0, 0.9%; 18:3, 0.2%; 20:4, 0.3%; 20:5, 0.9%; 22:4, 6.7%; 22:6, 36.1%; and others, 9.3%. The salinity range for growth of Schizochytrium isolates was from 0-30‰ with optimum salinity for growth between 20-30‰. |
| first_indexed | 2024-12-13T17:01:47Z |
| format | Article |
| id | doaj.art-099c4a3d483442c29b5dbeab26c5a802 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-13T17:01:47Z |
| publishDate | 2003-09-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-099c4a3d483442c29b5dbeab26c5a8022022-12-21T23:37:47ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952003-09-01255643650Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangroveK.W. FanNiyom KamlangdeeFive Schizochytrium strains (N-1, N-2, N-5, N-6, and N-9) were isolated from fallen, senescent leaves of mangrove tree (Kandelia candel) in Hong Kong. The fungi were cultivated in glucose yeast extract medium containing 60 g of glucose, 10 g of yeast extract and 1 L of 15‰ artificial seawater, initial pH 6.0, with shaking for 52 hr at 25ºC. Biomass yields of 5 isolates ranged from 10.8 to 13.2 g/l. Isolate N-2 yielding the highest dried cell mass at 13.2 g/l and isolate N-9 grew poorly with 10.8 g/l of biomass. EPA (Eicosapentaenoic acid, 20:5n-3) yield was low in most strains, while DHA (Docosahexaenoic acid, 22:6n-3) was high on the same medium. The contents of DHA in biomass varied: 174.9, 203.6, 186.1, 171.3 and 157.9 mg/g of dried-biomass for Schizochytrium isolate N-1, N-2, N-5, N-6, and N-9, respectively. Isolate N-2 had the highest proportion of DHA in fatty acid profile with 15:0, 28.7%; 16:0, 21.3%; 18:0, 0.9%; 18:3, 0.2%; 20:4, 0.3%; 20:5, 0.9%; 22:4, 6.7%; 22:6, 36.1%; and others, 9.3%. The salinity range for growth of Schizochytrium isolates was from 0-30‰ with optimum salinity for growth between 20-30‰.http://www.sjst.psu.ac.th/journal/25-5-pdf/11fatty-acid.pdfSchizochytriumdocosahexaenoic acid (DHA)polyunsaturated fatty acidThraustochytrids |
| spellingShingle | K.W. Fan Niyom Kamlangdee Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangrove Songklanakarin Journal of Science and Technology (SJST) Schizochytrium docosahexaenoic acid (DHA) polyunsaturated fatty acid Thraustochytrids |
| title | Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangrove |
| title_full | Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangrove |
| title_fullStr | Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangrove |
| title_full_unstemmed | Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangrove |
| title_short | Polyunsaturated fatty acids production by Schizochytrium sp. isolated from mangrove |
| title_sort | polyunsaturated fatty acids production by schizochytrium sp isolated from mangrove |
| topic | Schizochytrium docosahexaenoic acid (DHA) polyunsaturated fatty acid Thraustochytrids |
| url | http://www.sjst.psu.ac.th/journal/25-5-pdf/11fatty-acid.pdf |
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