In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe
Kaempferia angustifolia Roscoe is a medicinal plant in the family Zingiberaceae, with wild occurrence decreasing as a result of both natural and human threats. Tissue culture techniques provide an alternative method of propagation for mass production. Microshoots of K. angustifolia (1 cm in length)...
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| Format: | Article |
| Language: | English |
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AcademicPres
2023-11-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13190 |
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| author | Surapon SAENSOUK Wipa YAOWACHAI Theeraphan CHUMROENPHAT Sukanya NONTHALEE Piyaporn SAENSOUK |
| author_facet | Surapon SAENSOUK Wipa YAOWACHAI Theeraphan CHUMROENPHAT Sukanya NONTHALEE Piyaporn SAENSOUK |
| author_sort | Surapon SAENSOUK |
| collection | DOAJ |
| description |
Kaempferia angustifolia Roscoe is a medicinal plant in the family Zingiberaceae, with wild occurrence decreasing as a result of both natural and human threats. Tissue culture techniques provide an alternative method of propagation for mass production. Microshoots of K. angustifolia (1 cm in length) were cultured on MS medium supplemented with various concentrations of cytokinins (BA and Kinetin) and auxins (NAA and IAA) for 8 weeks. Phytochemical profiles were evaluated by total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ⁄ antioxidant power (FRAP) assay) and Gas chromatography–mass spectrometry (GC–MS) analysis. Maximum number of shoots was observed in medium supplemented with 2 mg/l BA plus 0.5 mg/l NAA (6.33 shoots/explant) and optimal rooting was induced in MS medium with 2 mg/l Kinetin plus 1 mg/l NAA (35.70 roots/explant). Highest numbers of shoots and roots were obtained when cultured on liquid MS medium supplemented with 2 mg/l BA, 2 mg/l Kinetin and 1 mg/l NAA (8.73 shoots/explant and 29.67 roots/explant, respectively). Regenerated plantlets of K. angustifolia were transferred to pots containing different types of plant materials under natural conditions for 8 weeks. Optimal survival rate was 100% when transplanting K. angustifolia to soil, sand, soil and sand (1:1) or soil and small pieces of rock (1:1) ratio. The methanol extract of leaves K. angustifolia from natural plants and in vitro derives plants showed a significantly higher amount of TFC and antioxidant activity. GC-MS analysis identified 52 phytochemical compounds in leaves of K. angustifolia. This study may be helpful to increase the value of commercial production of K. angustifolia, pharmaceutical and medicinal purpose.
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| first_indexed | 2024-03-08T20:14:39Z |
| format | Article |
| id | doaj.art-09a5f3afb6b24df19fae7f6b49d58de5 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-03-08T20:14:39Z |
| publishDate | 2023-11-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-09a5f3afb6b24df19fae7f6b49d58de52023-12-22T16:11:55ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092023-11-0151410.15835/nbha51413190In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia RoscoeSurapon SAENSOUK0Wipa YAOWACHAI1Theeraphan CHUMROENPHAT2Sukanya NONTHALEE3Piyaporn SAENSOUK4Mahasarakham University, Walai Rukhavej Botanical Research Institute, Mahasarakham 44150; Mahasarakham University, Diversity of Family Zingiberaceae and Vascular Plant for Its Applications Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Science, Department of Biology, Mahasarakham 44150Ubon Ratchathani Rajabhat University, Faculty of Thai Traditional and Alternative Medicine, Aesthetic Sciences and Health Program, Ubonratchathani 34000Horticultural Research Institute, Trang Horticultural Research Center, Trang 92150Mahasarakham University, Faculty of Science, Department of Biology, Mahasarakham 44150; Mahasarakham University, Diversity of Family Zingiberaceae and Vascular Plant for Its Applications Research Unit, Mahasarakham 44150 Kaempferia angustifolia Roscoe is a medicinal plant in the family Zingiberaceae, with wild occurrence decreasing as a result of both natural and human threats. Tissue culture techniques provide an alternative method of propagation for mass production. Microshoots of K. angustifolia (1 cm in length) were cultured on MS medium supplemented with various concentrations of cytokinins (BA and Kinetin) and auxins (NAA and IAA) for 8 weeks. Phytochemical profiles were evaluated by total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ⁄ antioxidant power (FRAP) assay) and Gas chromatography–mass spectrometry (GC–MS) analysis. Maximum number of shoots was observed in medium supplemented with 2 mg/l BA plus 0.5 mg/l NAA (6.33 shoots/explant) and optimal rooting was induced in MS medium with 2 mg/l Kinetin plus 1 mg/l NAA (35.70 roots/explant). Highest numbers of shoots and roots were obtained when cultured on liquid MS medium supplemented with 2 mg/l BA, 2 mg/l Kinetin and 1 mg/l NAA (8.73 shoots/explant and 29.67 roots/explant, respectively). Regenerated plantlets of K. angustifolia were transferred to pots containing different types of plant materials under natural conditions for 8 weeks. Optimal survival rate was 100% when transplanting K. angustifolia to soil, sand, soil and sand (1:1) or soil and small pieces of rock (1:1) ratio. The methanol extract of leaves K. angustifolia from natural plants and in vitro derives plants showed a significantly higher amount of TFC and antioxidant activity. GC-MS analysis identified 52 phytochemical compounds in leaves of K. angustifolia. This study may be helpful to increase the value of commercial production of K. angustifolia, pharmaceutical and medicinal purpose. https://www.notulaebotanicae.ro/index.php/nbha/article/view/13190antioxidantGC-MSKaempferia angustifoliamicropropagationTFCTPC |
| spellingShingle | Surapon SAENSOUK Wipa YAOWACHAI Theeraphan CHUMROENPHAT Sukanya NONTHALEE Piyaporn SAENSOUK In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe Notulae Botanicae Horti Agrobotanici Cluj-Napoca antioxidant GC-MS Kaempferia angustifolia micropropagation TFC TPC |
| title | In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe |
| title_full | In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe |
| title_fullStr | In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe |
| title_full_unstemmed | In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe |
| title_short | In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe |
| title_sort | in vitro regeneration transplantation and phytochemical profiles of kaempferia angustifolia roscoe |
| topic | antioxidant GC-MS Kaempferia angustifolia micropropagation TFC TPC |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13190 |
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