Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor Mice
Myeloid-derived suppressor cells (MDSCs) are capable of suppressing the immune response. 5-Fluorouracil (5-FU) compared to other chemotherapy drugs have shown considerable decreases in the number of MDSCs without visible effects on T, B and natural killer cells, as well as dendritic cells (DCs). DC-...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2018-02-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1331 |
| _version_ | 1811277666025734144 |
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| author | Najmeh khosravianfar Jamshid Hadjati Afshin Namdar Roobina Boghozian Morteza Hafezi Mahboubeh Ashourpour Nasim Kheshtchin Mahsa Banitalebi Reza Mirzaei Seyed Alireza Razavi |
| author_facet | Najmeh khosravianfar Jamshid Hadjati Afshin Namdar Roobina Boghozian Morteza Hafezi Mahboubeh Ashourpour Nasim Kheshtchin Mahsa Banitalebi Reza Mirzaei Seyed Alireza Razavi |
| author_sort | Najmeh khosravianfar |
| collection | DOAJ |
| description | Myeloid-derived suppressor cells (MDSCs) are capable of suppressing the immune response. 5-Fluorouracil (5-FU) compared to other chemotherapy drugs have shown considerable decreases in the number of MDSCs without visible effects on T, B and natural killer cells, as well as dendritic cells (DCs). DC-based vaccines considered to be appropriate candidates for cancer immunotherapy. However, due to the presence of various factors like MDSCs in tumor microenvironment, DC vaccine cannot effectively perform its function. The purpose of this study was to evaluate the effect of low doses of 5-FU on the efficacy of DC-based vaccines in preventing and treating of melanoma tumor model. This research was performed on 28 melanoma tumor bearing C57BL/6 female mice. The mice were randomly divided to 4 groups, group 1 is control population while group 2 and 3 were treated with DC vaccine and 5-FU respectively and group 4 was treated with both DC Vaccine and 5-FU. The mice survival, tumor growth rate, number of MDSC and CD8+/ CD107a+ T cells in mice spleen were evaluated in each group with maximum result in group 4. Our results revealed that combination of DC vaccine and 5-FU reduced number of MDSCs (3%) and also tumor growth rate(10%)(p<0.05) and increased mice survival (70%) and increased CD8+ /CD107a+ T cells (25%). This study have shown that combinational therapy with DC vaccine improved immunity in tumor mice compared to the therapy consisting of DC vaccine or 5-FU only. |
| first_indexed | 2024-04-13T00:20:52Z |
| format | Article |
| id | doaj.art-09ac869cebf6409d8efd0bc7ad304a3e |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-04-13T00:20:52Z |
| publishDate | 2018-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-09ac869cebf6409d8efd0bc7ad304a3e2022-12-22T03:10:46ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492018-02-011711331Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor MiceNajmeh khosravianfar0Jamshid Hadjati1Afshin Namdar2Roobina Boghozian3Morteza Hafezi4Mahboubeh Ashourpour5Nasim Kheshtchin6Mahsa Banitalebi7Reza Mirzaei8Seyed Alireza Razavi9Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranMyeloid-derived suppressor cells (MDSCs) are capable of suppressing the immune response. 5-Fluorouracil (5-FU) compared to other chemotherapy drugs have shown considerable decreases in the number of MDSCs without visible effects on T, B and natural killer cells, as well as dendritic cells (DCs). DC-based vaccines considered to be appropriate candidates for cancer immunotherapy. However, due to the presence of various factors like MDSCs in tumor microenvironment, DC vaccine cannot effectively perform its function. The purpose of this study was to evaluate the effect of low doses of 5-FU on the efficacy of DC-based vaccines in preventing and treating of melanoma tumor model. This research was performed on 28 melanoma tumor bearing C57BL/6 female mice. The mice were randomly divided to 4 groups, group 1 is control population while group 2 and 3 were treated with DC vaccine and 5-FU respectively and group 4 was treated with both DC Vaccine and 5-FU. The mice survival, tumor growth rate, number of MDSC and CD8+/ CD107a+ T cells in mice spleen were evaluated in each group with maximum result in group 4. Our results revealed that combination of DC vaccine and 5-FU reduced number of MDSCs (3%) and also tumor growth rate(10%)(p<0.05) and increased mice survival (70%) and increased CD8+ /CD107a+ T cells (25%). This study have shown that combinational therapy with DC vaccine improved immunity in tumor mice compared to the therapy consisting of DC vaccine or 5-FU only.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1331Dendritic cell5- FluorouracilMyeloid-derived suppressor cellTumor |
| spellingShingle | Najmeh khosravianfar Jamshid Hadjati Afshin Namdar Roobina Boghozian Morteza Hafezi Mahboubeh Ashourpour Nasim Kheshtchin Mahsa Banitalebi Reza Mirzaei Seyed Alireza Razavi Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor Mice Iranian Journal of Allergy, Asthma and Immunology Dendritic cell 5- Fluorouracil Myeloid-derived suppressor cell Tumor |
| title | Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor Mice |
| title_full | Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor Mice |
| title_fullStr | Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor Mice |
| title_full_unstemmed | Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor Mice |
| title_short | Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor Mice |
| title_sort | myeloid derived suppressor cells elimination by 5 fluorouracil increased dendritic cell based vaccine function and improved immunity in tumor mice |
| topic | Dendritic cell 5- Fluorouracil Myeloid-derived suppressor cell Tumor |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/1331 |
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