The Effect of α-Mangostin and Cisplatin on Ovarian Cancer Cells and the Microenvironment
Ovarian cancer is one of the cancers that, unfortunately, is detected at a late stage of development. The current use of treatment has many side effects. Notably, up to 20% of patients show cisplatin resistance. We assess the effects of cisplatin and/or α-mangostin, a natural plant derivative, on ov...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/5/1116 |
| _version_ | 1797501349449957376 |
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| author | Paulina Borzdziłowska Ilona Bednarek |
| author_facet | Paulina Borzdziłowska Ilona Bednarek |
| author_sort | Paulina Borzdziłowska |
| collection | DOAJ |
| description | Ovarian cancer is one of the cancers that, unfortunately, is detected at a late stage of development. The current use of treatment has many side effects. Notably, up to 20% of patients show cisplatin resistance. We assess the effects of cisplatin and/or α-mangostin, a natural plant derivative, on ovarian cancer cells and on the cancer cell microenvironment. The effect of cisplatin and/or α-mangostin on the following cells of ovarian cancer lines: A2780, TOV-21G, and SKOV-3 was verified using the XTT cytotoxicity assay. The separate and combined effects of tested drugs on ovarian cancer cell viability were assessed. We assessed the influence of chemotherapeutic agents on the possibility of modulating the microenvironment. For this purpose, we isolated exosomes from drug-treated and untreated ovarian cancer cells. We estimated the differences in the amounts of exosomes released from cancer cells (NTA technique). We also examined the effects of isolated exosome fractions on normal human cells (NHDF human fibroblast line). In the present study, we demonstrate that treatment of A2780, SKOV-3, and TOV-21G cells with α-mangostin in combination with cisplatin can allow a reduction in cisplatin concentration while maintaining the same cytotoxic effect. Ovarian cancer cells release a variable number of exosomes into the microenvironment when exposed to α-mangostin and/or cisplatin. However, it is important to note that the cargo carried by exosomes released from drug-treated cells may be significantly different. |
| first_indexed | 2024-03-10T03:17:07Z |
| format | Article |
| id | doaj.art-09b0bb577b9f416d9b5434e3c93c3990 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T03:17:07Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-09b0bb577b9f416d9b5434e3c93c39902023-11-23T10:11:14ZengMDPI AGBiomedicines2227-90592022-05-01105111610.3390/biomedicines10051116The Effect of α-Mangostin and Cisplatin on Ovarian Cancer Cells and the MicroenvironmentPaulina Borzdziłowska0Ilona Bednarek1Department of Biotechnology and Genetic Engineering, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Biotechnology and Genetic Engineering, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, PolandOvarian cancer is one of the cancers that, unfortunately, is detected at a late stage of development. The current use of treatment has many side effects. Notably, up to 20% of patients show cisplatin resistance. We assess the effects of cisplatin and/or α-mangostin, a natural plant derivative, on ovarian cancer cells and on the cancer cell microenvironment. The effect of cisplatin and/or α-mangostin on the following cells of ovarian cancer lines: A2780, TOV-21G, and SKOV-3 was verified using the XTT cytotoxicity assay. The separate and combined effects of tested drugs on ovarian cancer cell viability were assessed. We assessed the influence of chemotherapeutic agents on the possibility of modulating the microenvironment. For this purpose, we isolated exosomes from drug-treated and untreated ovarian cancer cells. We estimated the differences in the amounts of exosomes released from cancer cells (NTA technique). We also examined the effects of isolated exosome fractions on normal human cells (NHDF human fibroblast line). In the present study, we demonstrate that treatment of A2780, SKOV-3, and TOV-21G cells with α-mangostin in combination with cisplatin can allow a reduction in cisplatin concentration while maintaining the same cytotoxic effect. Ovarian cancer cells release a variable number of exosomes into the microenvironment when exposed to α-mangostin and/or cisplatin. However, it is important to note that the cargo carried by exosomes released from drug-treated cells may be significantly different.https://www.mdpi.com/2227-9059/10/5/1116exosomesα-mangostincisplatinovarian cancerNTA—nanoparticle analysis tracking |
| spellingShingle | Paulina Borzdziłowska Ilona Bednarek The Effect of α-Mangostin and Cisplatin on Ovarian Cancer Cells and the Microenvironment Biomedicines exosomes α-mangostin cisplatin ovarian cancer NTA—nanoparticle analysis tracking |
| title | The Effect of α-Mangostin and Cisplatin on Ovarian Cancer Cells and the Microenvironment |
| title_full | The Effect of α-Mangostin and Cisplatin on Ovarian Cancer Cells and the Microenvironment |
| title_fullStr | The Effect of α-Mangostin and Cisplatin on Ovarian Cancer Cells and the Microenvironment |
| title_full_unstemmed | The Effect of α-Mangostin and Cisplatin on Ovarian Cancer Cells and the Microenvironment |
| title_short | The Effect of α-Mangostin and Cisplatin on Ovarian Cancer Cells and the Microenvironment |
| title_sort | effect of α mangostin and cisplatin on ovarian cancer cells and the microenvironment |
| topic | exosomes α-mangostin cisplatin ovarian cancer NTA—nanoparticle analysis tracking |
| url | https://www.mdpi.com/2227-9059/10/5/1116 |
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