Alveolar bone loss and mineralization in the pig with experimental periodontal disease
Objective: To address how experimental periodontal disease affects alveolar bone mass and mineral apposition in a young pig model. Materials and methods: Seven three-month-old pigs were periodically inoculated with 4 types of periodontal bacteria, along with a ligature around the last maxillary deci...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844018300793 |
| _version_ | 1828463549658169344 |
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| author | Mandee Yang Grace Eun Nam Atriya Salamati Michael Baldwin Mengzhao Deng Zi-Jun Liu |
| author_facet | Mandee Yang Grace Eun Nam Atriya Salamati Michael Baldwin Mengzhao Deng Zi-Jun Liu |
| author_sort | Mandee Yang |
| collection | DOAJ |
| description | Objective: To address how experimental periodontal disease affects alveolar bone mass and mineral apposition in a young pig model. Materials and methods: Seven three-month-old pigs were periodically inoculated with 4 types of periodontal bacteria, along with a ligature around the last maxillary deciduous molar for 8 weeks to induce periodontal disease (PG). Eight same-aged pigs served as the control (CG). Segmentations of 3D cone-beam CT images were performed to quantify volumes of the total alveolar bone, alveolar ridge, and all roots of the target molar. Calcein and alizarin were administered for labeling mineral apposition before euthanasia. The harvested molar blocks were sectioned and examined under epifluorescence. The inter-label distance between the two vital markers at regional bone surfaces were measured and mineral apposition rate (MAR) was calculated. Results: A significant reduction of total alveolar bone volume was seen in PG with the major loss at the alveolar ridge. MAR was significantly higher at the root furcation region than those at both buccal and palatal ridges in CG. Compared with CG, PG animals showed more interrupted labeled bands with significantly lower MAR at the furcation region. MARs were positively associated with both the volumes of total alveolar bone and ridge in CG, but only with the total alveolar bone in PG. Conclusions: In young growing pigs, mineral apposition is region specific. The experimental periodontal disease not only leads to alveolar bone loss, but also perturbs mineral apposition for new bone formation, thus impairing the homeostasis of alveolar bone remodeling. |
| first_indexed | 2024-12-11T02:55:29Z |
| format | Article |
| id | doaj.art-09b2078bc3394901a57e495a0c4e9c67 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-11T02:55:29Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-09b2078bc3394901a57e495a0c4e9c672022-12-22T01:23:09ZengElsevierHeliyon2405-84402018-03-0143e00589Alveolar bone loss and mineralization in the pig with experimental periodontal diseaseMandee Yang0Grace Eun Nam1Atriya Salamati2Michael Baldwin3Mengzhao Deng4Zi-Jun Liu5Dept. Orthodontics, School of Dentistry, University of Washington, Seattle, USADept. Orthodontics, School of Dentistry, University of Washington, Seattle, USADept. Orthodontics, School of Dentistry, University of Washington, Seattle, USA; Dept. Oral Health Sciences, School of Dentistry, University of Washington, Seattle, USADept. Orthodontics, School of Dentistry, University of Washington, Seattle, USA; Dept. Oral Health Sciences, School of Dentistry, University of Washington, Seattle, USADept. Orthodontics, School of Dentistry, University of Washington, Seattle, USA; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR ChinaDept. Orthodontics, School of Dentistry, University of Washington, Seattle, USA; Dept. Oral Health Sciences, School of Dentistry, University of Washington, Seattle, USA; Corresponding author.Objective: To address how experimental periodontal disease affects alveolar bone mass and mineral apposition in a young pig model. Materials and methods: Seven three-month-old pigs were periodically inoculated with 4 types of periodontal bacteria, along with a ligature around the last maxillary deciduous molar for 8 weeks to induce periodontal disease (PG). Eight same-aged pigs served as the control (CG). Segmentations of 3D cone-beam CT images were performed to quantify volumes of the total alveolar bone, alveolar ridge, and all roots of the target molar. Calcein and alizarin were administered for labeling mineral apposition before euthanasia. The harvested molar blocks were sectioned and examined under epifluorescence. The inter-label distance between the two vital markers at regional bone surfaces were measured and mineral apposition rate (MAR) was calculated. Results: A significant reduction of total alveolar bone volume was seen in PG with the major loss at the alveolar ridge. MAR was significantly higher at the root furcation region than those at both buccal and palatal ridges in CG. Compared with CG, PG animals showed more interrupted labeled bands with significantly lower MAR at the furcation region. MARs were positively associated with both the volumes of total alveolar bone and ridge in CG, but only with the total alveolar bone in PG. Conclusions: In young growing pigs, mineral apposition is region specific. The experimental periodontal disease not only leads to alveolar bone loss, but also perturbs mineral apposition for new bone formation, thus impairing the homeostasis of alveolar bone remodeling.http://www.sciencedirect.com/science/article/pii/S2405844018300793Dentistry |
| spellingShingle | Mandee Yang Grace Eun Nam Atriya Salamati Michael Baldwin Mengzhao Deng Zi-Jun Liu Alveolar bone loss and mineralization in the pig with experimental periodontal disease Heliyon Dentistry |
| title | Alveolar bone loss and mineralization in the pig with experimental periodontal disease |
| title_full | Alveolar bone loss and mineralization in the pig with experimental periodontal disease |
| title_fullStr | Alveolar bone loss and mineralization in the pig with experimental periodontal disease |
| title_full_unstemmed | Alveolar bone loss and mineralization in the pig with experimental periodontal disease |
| title_short | Alveolar bone loss and mineralization in the pig with experimental periodontal disease |
| title_sort | alveolar bone loss and mineralization in the pig with experimental periodontal disease |
| topic | Dentistry |
| url | http://www.sciencedirect.com/science/article/pii/S2405844018300793 |
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