Effect of a lytic bacteriophage on rabbits experimentally infected with pathogenic Escherichia coli
Pathogenic Escherichia coli (E. coli) is severely threatening the rabbit industry in China, and the concern over antibiotic-resistant bacteria has given rise to an urgent need for antibiotic alternatives. In this study, a member (ZRP1) of the Myoviridae family was isolated from rabbit faeces using a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitat Politècnica de València
2017-09-01
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| Series: | World Rabbit Science |
| Subjects: | |
| Online Access: | https://polipapers.upv.es/index.php/wrs/article/view/6395 |
| Summary: | Pathogenic Escherichia coli (E. coli) is severely threatening the rabbit industry in China, and the concern over antibiotic-resistant bacteria has given rise to an urgent need for antibiotic alternatives. In this study, a member (ZRP1) of the Myoviridae family was isolated from rabbit faeces using a strain of rabbit atypical enteropathogenic E. coli (ZR1) as host. The one-step growth curve indicated that the latent period was around 25 to 30 min and the burst size was 144±31 plaque-forming unit/cell. The rate of phage-resistant mutation was 7×10–5±4×10–5. When the bacteriophage input at the multiplicity of infection (MOI) was 0.1, 1 or 10, the growth of host E. coli in broth was inhibited for 5 h. A single intravenous injection of ZRP1 at MOI 0.1, 1 or 10 significantly prolonged the survival time of rabbits which simultaneously received a lethal dose of ZR1. |
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| ISSN: | 1257-5011 1989-8886 |