Effect of shilajit on the levels of pro-inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male rats
Background: The levels of IL-13, IL-4, IL-1B, TNF-α and IL-10 alter in the colon of people with ulcerative colitis. previous studies, it was found that shilajit (asphaltum) was effective in the improvement of ulcerative colitis.we guessed that the shilajit has been able to improve the ulcerative col...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institue of Medicinal Plants, ACECR
2020-09-01
|
| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-2376-en.html |
| _version_ | 1818360137910321152 |
|---|---|
| author | Fatemeh Alimahdi Nader Shahrokhi Mohammad Khaksari Hadad Gholamerza Asadikaram Moslem Abolhasani |
| author_facet | Fatemeh Alimahdi Nader Shahrokhi Mohammad Khaksari Hadad Gholamerza Asadikaram Moslem Abolhasani |
| author_sort | Fatemeh Alimahdi |
| collection | DOAJ |
| description | Background: The levels of IL-13, IL-4, IL-1B, TNF-α and IL-10 alter in the colon of people with ulcerative colitis. previous studies, it was found that shilajit (asphaltum) was effective in the improvement of ulcerative colitis.we guessed that the shilajit has been able to improve the ulcerative colitis by affecting the amount of cytokines. Objective: The aim of the present study was to investigate the effects of shilajit on IL-13, IL-4, IL-1B, TNF-α and IL-10 in ulcerative colitis. Methods: In this experimental study, 49 white male rats were randomly divided into 7 groups including sham groups, ulcerative colitis, Gavage vehicle, Gavage shilajit, sulfasalazine, Intra Anal Vehicle, Intra Anal shilajit. To produce ulcerative colitis 2 cc acetic acid (4%) was prescribed intra-rectal. 4 days after the induction of colitis, it was given 4 days: shilajit 250 mg / kg was used as a gavage or intra anal. Sulfasalazine was dosed at 250 mg / kg in the form of gavage. Results: The Gavage shilajit group increased IL-10, IL-4and decreased IL-1β, TNF-α compared to Gavage vehicle group. Shilajit has been able to close the levels of IL-4, IL-13, IL-10, TNF-α to normal values of the sham group. Conclusion: Considering that the weight ratio of intestinal rat to the body of rat in the shilajit groups was lower than that of the sulfasalazine group, it can be shown that shilajit has been able to more reduce inflammation by making a more suitable change in cytokines in comparison with sulfasalazine. |
| first_indexed | 2024-12-13T20:56:01Z |
| format | Article |
| id | doaj.art-09b90274a75b4a1bb94cce3a42d797ec |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-13T20:56:01Z |
| publishDate | 2020-09-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-09b90274a75b4a1bb94cce3a42d797ec2022-12-21T23:31:45ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582020-09-0119757891Effect of shilajit on the levels of pro-inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male ratsFatemeh Alimahdi0Nader Shahrokhi1Mohammad Khaksari Hadad2Gholamerza Asadikaram3Moslem Abolhasani4 Kerman University of Medical Sciences, Kerman, Iran Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, University of Medical Sciences, Kerman, Iran Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran Background: The levels of IL-13, IL-4, IL-1B, TNF-α and IL-10 alter in the colon of people with ulcerative colitis. previous studies, it was found that shilajit (asphaltum) was effective in the improvement of ulcerative colitis.we guessed that the shilajit has been able to improve the ulcerative colitis by affecting the amount of cytokines. Objective: The aim of the present study was to investigate the effects of shilajit on IL-13, IL-4, IL-1B, TNF-α and IL-10 in ulcerative colitis. Methods: In this experimental study, 49 white male rats were randomly divided into 7 groups including sham groups, ulcerative colitis, Gavage vehicle, Gavage shilajit, sulfasalazine, Intra Anal Vehicle, Intra Anal shilajit. To produce ulcerative colitis 2 cc acetic acid (4%) was prescribed intra-rectal. 4 days after the induction of colitis, it was given 4 days: shilajit 250 mg / kg was used as a gavage or intra anal. Sulfasalazine was dosed at 250 mg / kg in the form of gavage. Results: The Gavage shilajit group increased IL-10, IL-4and decreased IL-1β, TNF-α compared to Gavage vehicle group. Shilajit has been able to close the levels of IL-4, IL-13, IL-10, TNF-α to normal values of the sham group. Conclusion: Considering that the weight ratio of intestinal rat to the body of rat in the shilajit groups was lower than that of the sulfasalazine group, it can be shown that shilajit has been able to more reduce inflammation by making a more suitable change in cytokines in comparison with sulfasalazine.http://jmp.ir/article-1-2376-en.htmlasphaltumcytokineshilajitsulfasalazinulcerative colitis |
| spellingShingle | Fatemeh Alimahdi Nader Shahrokhi Mohammad Khaksari Hadad Gholamerza Asadikaram Moslem Abolhasani Effect of shilajit on the levels of pro-inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male rats Journal of Medicinal Plants asphaltum cytokine shilajit sulfasalazin ulcerative colitis |
| title | Effect of shilajit on the levels of pro-inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male rats |
| title_full | Effect of shilajit on the levels of pro-inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male rats |
| title_fullStr | Effect of shilajit on the levels of pro-inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male rats |
| title_full_unstemmed | Effect of shilajit on the levels of pro-inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male rats |
| title_short | Effect of shilajit on the levels of pro-inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male rats |
| title_sort | effect of shilajit on the levels of pro inflammatory and anti inflammatory cytokines in ulcerative colitis induced by acetic acid in male rats |
| topic | asphaltum cytokine shilajit sulfasalazin ulcerative colitis |
| url | http://jmp.ir/article-1-2376-en.html |
| work_keys_str_mv | AT fatemehalimahdi effectofshilajitonthelevelsofproinflammatoryandantiinflammatorycytokinesinulcerativecolitisinducedbyaceticacidinmalerats AT nadershahrokhi effectofshilajitonthelevelsofproinflammatoryandantiinflammatorycytokinesinulcerativecolitisinducedbyaceticacidinmalerats AT mohammadkhaksarihadad effectofshilajitonthelevelsofproinflammatoryandantiinflammatorycytokinesinulcerativecolitisinducedbyaceticacidinmalerats AT gholamerzaasadikaram effectofshilajitonthelevelsofproinflammatoryandantiinflammatorycytokinesinulcerativecolitisinducedbyaceticacidinmalerats AT moslemabolhasani effectofshilajitonthelevelsofproinflammatoryandantiinflammatorycytokinesinulcerativecolitisinducedbyaceticacidinmalerats |