Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia
Introduction: Acute lymphocytic leukemia (ALL) is the most common cancer type in children and accounts for 80% of pediatric leukemias. Novel targets are necessary to improve survival rates for refractory and relapsed disease. There is accumulating evidence that Toll-like Receptor (TLR) signaling ma...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Câncer (INCA)
2023-07-01
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| Series: | Revista Brasileira de Cancerologia |
| Subjects: | |
| Online Access: | https://rbc.inca.gov.br/index.php/revista/article/view/3676 |
| _version_ | 1797280288757252096 |
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| author | Matheus Loureiro da Silva Cruz Rafael Pereira dos Santos Barbara Kunzler Souza Mariane da Cunha Jaeger Camila Alves da Silva Lauro José Gregianin Jiseh Fagundes Loss Rebeca Ferreira Marques Algemir Lunardi Brunetto André Tesainer Brunetto Rafael Roesler Caroline Brunetto de Farias |
| author_facet | Matheus Loureiro da Silva Cruz Rafael Pereira dos Santos Barbara Kunzler Souza Mariane da Cunha Jaeger Camila Alves da Silva Lauro José Gregianin Jiseh Fagundes Loss Rebeca Ferreira Marques Algemir Lunardi Brunetto André Tesainer Brunetto Rafael Roesler Caroline Brunetto de Farias |
| author_sort | Matheus Loureiro da Silva Cruz |
| collection | DOAJ |
| description |
Introduction: Acute lymphocytic leukemia (ALL) is the most common cancer type in children and accounts for 80% of pediatric leukemias. Novel targets are necessary to improve survival rates for refractory and relapsed disease. There is accumulating evidence that Toll-like Receptor (TLR) signaling may be associated with outcomes in cancer however little has been described in leukemias. Objective: Analyze the expression and contribution of TLRs to the development of childhood ALL. Method: To evaluate the effect of specific TLR2, TLR3, and TLR4 agonists on the viability and proliferation of childhood ALL cell lines and to analyzed the mRNA expression of these types of TLR in bone marrow blast cells at diagnosis (D0) and induction (D35) in pediatric ALL patients. Results: Treatment with TLR agonists reduced the cell viability of Jurkat and Sup-B15 cell lines. Cell cycle distribution in Jurkat was altered, reducing polyploid cells and increasing sub-G1 phase. Conclusion: It was observed that the cell viability of the cell lines responded with different sensitivities to the agonists. The polyploidy associated with tumor malignancy was reduced, in addition to the increase in the sub-G1 phase indicating an increase in apoptosis. There were differences in TLR expression at D35 between groups at risk of the disease. Patients with high expression of TLR2 and low expression of TLR4 on D35 demonstrated a worse prognosis.
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| first_indexed | 2024-03-07T16:37:59Z |
| format | Article |
| id | doaj.art-09ba00fd63214fcdbaea678fe9b6ef79 |
| institution | Directory Open Access Journal |
| issn | 2176-9745 |
| language | English |
| last_indexed | 2024-03-07T16:37:59Z |
| publishDate | 2023-07-01 |
| publisher | Instituto Nacional de Câncer (INCA) |
| record_format | Article |
| series | Revista Brasileira de Cancerologia |
| spelling | doaj.art-09ba00fd63214fcdbaea678fe9b6ef792024-03-03T09:09:04ZengInstituto Nacional de Câncer (INCA)Revista Brasileira de Cancerologia2176-97452023-07-0169310.32635/2176-9745.RBC.2023v69n3.3676Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic LeukemiaMatheus Loureiro da Silva Cruz0Rafael Pereira dos Santos1Barbara Kunzler Souza2Mariane da Cunha Jaeger3Camila Alves da Silva4Lauro José Gregianin5Jiseh Fagundes Loss6Rebeca Ferreira Marques7Algemir Lunardi Brunetto8André Tesainer Brunetto9Rafael Roesler10Caroline Brunetto de Farias11Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil.Universidade Federal do Rio Grande do Sul (UFRGS). Porto Alegre (RS), Brazil. Instituto do Câncer Infantil. Porto Alegre (RS), Brazil. Introduction: Acute lymphocytic leukemia (ALL) is the most common cancer type in children and accounts for 80% of pediatric leukemias. Novel targets are necessary to improve survival rates for refractory and relapsed disease. There is accumulating evidence that Toll-like Receptor (TLR) signaling may be associated with outcomes in cancer however little has been described in leukemias. Objective: Analyze the expression and contribution of TLRs to the development of childhood ALL. Method: To evaluate the effect of specific TLR2, TLR3, and TLR4 agonists on the viability and proliferation of childhood ALL cell lines and to analyzed the mRNA expression of these types of TLR in bone marrow blast cells at diagnosis (D0) and induction (D35) in pediatric ALL patients. Results: Treatment with TLR agonists reduced the cell viability of Jurkat and Sup-B15 cell lines. Cell cycle distribution in Jurkat was altered, reducing polyploid cells and increasing sub-G1 phase. Conclusion: It was observed that the cell viability of the cell lines responded with different sensitivities to the agonists. The polyploidy associated with tumor malignancy was reduced, in addition to the increase in the sub-G1 phase indicating an increase in apoptosis. There were differences in TLR expression at D35 between groups at risk of the disease. Patients with high expression of TLR2 and low expression of TLR4 on D35 demonstrated a worse prognosis. https://rbc.inca.gov.br/index.php/revista/article/view/3676precursor cell lymphoblastic leukemia-lymphomaToll-like receptors |
| spellingShingle | Matheus Loureiro da Silva Cruz Rafael Pereira dos Santos Barbara Kunzler Souza Mariane da Cunha Jaeger Camila Alves da Silva Lauro José Gregianin Jiseh Fagundes Loss Rebeca Ferreira Marques Algemir Lunardi Brunetto André Tesainer Brunetto Rafael Roesler Caroline Brunetto de Farias Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia Revista Brasileira de Cancerologia precursor cell lymphoblastic leukemia-lymphoma Toll-like receptors |
| title | Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia |
| title_full | Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia |
| title_fullStr | Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia |
| title_full_unstemmed | Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia |
| title_short | Toll-like Receptors 2, 3, and 4 in Childhood Acute Lymphocytic Leukemia |
| title_sort | toll like receptors 2 3 and 4 in childhood acute lymphocytic leukemia |
| topic | precursor cell lymphoblastic leukemia-lymphoma Toll-like receptors |
| url | https://rbc.inca.gov.br/index.php/revista/article/view/3676 |
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