Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans
Background and Aim: Sialic acid-binding immunoglobulin-like lectin 1 (Siglec-1) is a transmembrane glycoprotein involved in the sialic acid (Sia)-dependent regulation of the immune system. Siglec-1 expression has recently been identified in the male reproductive tract (MRT) of several species, inclu...
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| Format: | Article |
| Language: | English |
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Veterinary World
2024-03-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.17/March-2024/16.pdf |
| _version_ | 1797253683795197952 |
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| author | Hazem Almhanna Arun HS Kumar David Kilroy Gina Duggan Jane A. Irwin Bridget Hogg Colm Reid |
| author_facet | Hazem Almhanna Arun HS Kumar David Kilroy Gina Duggan Jane A. Irwin Bridget Hogg Colm Reid |
| author_sort | Hazem Almhanna |
| collection | DOAJ |
| description | Background and Aim: Sialic acid-binding immunoglobulin-like lectin 1 (Siglec-1) is a transmembrane glycoprotein involved in the sialic acid (Sia)-dependent regulation of the immune system. Siglec-1 expression has recently been identified in the male reproductive tract (MRT) of several species, including humans, cattle, horses, and sheep, and may play a role in modulating fertility in a Sia-dependent manner.
Materials and Methods: In this study, protein–protein interaction (PPI) analysis of Siglec-1 was conducted to identify associated network protein conservation, and the expression of Siglec-1 in the MRT of mice and rats, including their accessory sex glands and spermatozoa was determined by immunostaining.
Results: Network analysis of proteins with Siglec-1 in mice and rats demonstrated significant similarity to human Siglec-1 networks, suggesting a similar conservation of network proteins between these species and, hence, a potential conservation role in immune modulation and function. Specific immunostaining patterns of mouse and rat testes, epididymis, ductus deferens, accessory sex gland tissues, and sperm were detected using human Siglec-1. These results confirmed that the human Siglec-1 antibody could cross-react with mouse and rat Siglec-1, suggesting that the specific expression patterns of Siglec-1 in the MRT and sperm of both mice and rats are similar to those observed in other species.
Conclusion: The conservation of Siglec-1 expression patterns in sperm and within the MRT and the similarity of protein networks for Siglec-1 across species suggest that Siglec-1 may function in a similar manner across species. These results also suggest that rodents may serve as a valuable model system for exploring the function of Siglecs in the reproductive system across species and their potential role in modulating fertility in a Sia-dependent manner. |
| first_indexed | 2024-04-24T21:37:58Z |
| format | Article |
| id | doaj.art-09bdb9cf92aa43fcb476f6d9b47fc26c |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-24T21:37:58Z |
| publishDate | 2024-03-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-09bdb9cf92aa43fcb476f6d9b47fc26c2024-03-21T12:13:44ZengVeterinary WorldVeterinary World0972-89882231-09162024-03-0117364565710.14202/vetworld.2024.645-657Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humansHazem Almhanna0https://orcid.org/0000-0002-0189-2798Arun HS Kumar1https://orcid.org/0000-0001-8422-0219David Kilroy2Gina Duggan3https://orcid.org/0000-0002-1630-993XJane A. Irwin4https://orcid.org/0000-0002-1843-3643Bridget Hogg5https://orcid.org/0009-0005-4030-7116Colm Reid6https://orcid.org/0000-0001-9052-3291Department of Anatomy and Histology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq; School of Veterinary Medicine, University College Dublin, Belfield, Dublin-04, Ireland.School of Veterinary Medicine, University College Dublin, Belfield, Dublin-04, Ireland.School of Veterinary Medicine, University College Dublin, Belfield, Dublin-04, Ireland.School of Veterinary Medicine, University College Dublin, Belfield, Dublin-04, Ireland.School of Veterinary Medicine, University College Dublin, Belfield, Dublin-04, Ireland.School of Veterinary Medicine, University College Dublin, Belfield, Dublin-04, Ireland.School of Veterinary Medicine, University College Dublin, Belfield, Dublin-04, Ireland.Background and Aim: Sialic acid-binding immunoglobulin-like lectin 1 (Siglec-1) is a transmembrane glycoprotein involved in the sialic acid (Sia)-dependent regulation of the immune system. Siglec-1 expression has recently been identified in the male reproductive tract (MRT) of several species, including humans, cattle, horses, and sheep, and may play a role in modulating fertility in a Sia-dependent manner. Materials and Methods: In this study, protein–protein interaction (PPI) analysis of Siglec-1 was conducted to identify associated network protein conservation, and the expression of Siglec-1 in the MRT of mice and rats, including their accessory sex glands and spermatozoa was determined by immunostaining. Results: Network analysis of proteins with Siglec-1 in mice and rats demonstrated significant similarity to human Siglec-1 networks, suggesting a similar conservation of network proteins between these species and, hence, a potential conservation role in immune modulation and function. Specific immunostaining patterns of mouse and rat testes, epididymis, ductus deferens, accessory sex gland tissues, and sperm were detected using human Siglec-1. These results confirmed that the human Siglec-1 antibody could cross-react with mouse and rat Siglec-1, suggesting that the specific expression patterns of Siglec-1 in the MRT and sperm of both mice and rats are similar to those observed in other species. Conclusion: The conservation of Siglec-1 expression patterns in sperm and within the MRT and the similarity of protein networks for Siglec-1 across species suggest that Siglec-1 may function in a similar manner across species. These results also suggest that rodents may serve as a valuable model system for exploring the function of Siglecs in the reproductive system across species and their potential role in modulating fertility in a Sia-dependent manner.https://www.veterinaryworld.org/Vol.17/March-2024/16.pdffemale reproductive tractmale reproductive tractprotein–protein interactionsialic acidsialic acid-binding immunoglobulin-like lectin-1 |
| spellingShingle | Hazem Almhanna Arun HS Kumar David Kilroy Gina Duggan Jane A. Irwin Bridget Hogg Colm Reid Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans Veterinary World female reproductive tract male reproductive tract protein–protein interaction sialic acid sialic acid-binding immunoglobulin-like lectin-1 |
| title | Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans |
| title_full | Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans |
| title_fullStr | Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans |
| title_full_unstemmed | Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans |
| title_short | Comparison of Siglec-1 protein networks and expression patterns in sperm and male reproductive tracts of mice, rats, and humans |
| title_sort | comparison of siglec 1 protein networks and expression patterns in sperm and male reproductive tracts of mice rats and humans |
| topic | female reproductive tract male reproductive tract protein–protein interaction sialic acid sialic acid-binding immunoglobulin-like lectin-1 |
| url | https://www.veterinaryworld.org/Vol.17/March-2024/16.pdf |
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