Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and Pharmacokinetics
The aim of this study was to develop magnetic molecularly imprinted nano-conjugate sorbent for effective dispersive solid phase extraction of antazoline (ANT) and its metabolite, hydroxyantazoline (ANT-OH) in analytical method employing liquid chromatography coupled with mass spectrometry method. Th...
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| author | Joanna Giebułtowicz Natalia Korytowska Monika Sobiech Sebastian Polak Barbara Wiśniowska Roman Piotrowski Piotr Kułakowski Piotr Luliński |
| author_facet | Joanna Giebułtowicz Natalia Korytowska Monika Sobiech Sebastian Polak Barbara Wiśniowska Roman Piotrowski Piotr Kułakowski Piotr Luliński |
| author_sort | Joanna Giebułtowicz |
| collection | DOAJ |
| description | The aim of this study was to develop magnetic molecularly imprinted nano-conjugate sorbent for effective dispersive solid phase extraction of antazoline (ANT) and its metabolite, hydroxyantazoline (ANT-OH) in analytical method employing liquid chromatography coupled with mass spectrometry method. The core–shell material was characterized in terms of adsorption properties, morphology and structure. The heterogeneous population of adsorption sites towards ANT-OH was characterized by two K<sub>d</sub> and two B<sub>max</sub> values: K<sub>d</sub> (1) = 0.319 µg L<sup>−1</sup> and B<sub>max</sub> (1) = 0.240 μg g<sup>−1</sup>, and K<sub>d</sub> (2) = 34.6 µg L<sup>−1</sup> and B<sub>max</sub> (2) = 5.82 μg g<sup>−1</sup>. The elemental composition of magnetic sorbent was as follows: 17.55, 37.33, 9.14, 34.94 wt% for Si, C, Fe and O, respectively. The extraction protocol was optimized, and the obtained results were explained using theoretical analysis. Finally, the analytical method was validated prior to application to pharmacokinetic study in which the ANT was administrated intravenously to three healthy volunteers. The results prove that the novel sorbent could be useful in extraction of ANT and ANT-OH from human plasma and that the analytical strategy could be a versatile tool to explain a potential and pharmacological activity of ANT and ANT-OH. |
| first_indexed | 2024-03-10T12:42:11Z |
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| language | English |
| last_indexed | 2024-03-10T12:42:11Z |
| publishDate | 2021-04-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-09c0f7ad3925406e96fb0b716b464ea52023-11-21T13:45:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01227366510.3390/ijms22073665Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and PharmacokineticsJoanna Giebułtowicz0Natalia Korytowska1Monika Sobiech2Sebastian Polak3Barbara Wiśniowska4Roman Piotrowski5Piotr Kułakowski6Piotr Luliński7Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Social Pharmacy, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Social Pharmacy, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, PolandDepartment of Cardiology, Postgraduate Medical School, Grochowski Hospital, 04-073 Warsaw, PolandDepartment of Cardiology, Postgraduate Medical School, Grochowski Hospital, 04-073 Warsaw, PolandDepartment of Organic Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandThe aim of this study was to develop magnetic molecularly imprinted nano-conjugate sorbent for effective dispersive solid phase extraction of antazoline (ANT) and its metabolite, hydroxyantazoline (ANT-OH) in analytical method employing liquid chromatography coupled with mass spectrometry method. The core–shell material was characterized in terms of adsorption properties, morphology and structure. The heterogeneous population of adsorption sites towards ANT-OH was characterized by two K<sub>d</sub> and two B<sub>max</sub> values: K<sub>d</sub> (1) = 0.319 µg L<sup>−1</sup> and B<sub>max</sub> (1) = 0.240 μg g<sup>−1</sup>, and K<sub>d</sub> (2) = 34.6 µg L<sup>−1</sup> and B<sub>max</sub> (2) = 5.82 μg g<sup>−1</sup>. The elemental composition of magnetic sorbent was as follows: 17.55, 37.33, 9.14, 34.94 wt% for Si, C, Fe and O, respectively. The extraction protocol was optimized, and the obtained results were explained using theoretical analysis. Finally, the analytical method was validated prior to application to pharmacokinetic study in which the ANT was administrated intravenously to three healthy volunteers. The results prove that the novel sorbent could be useful in extraction of ANT and ANT-OH from human plasma and that the analytical strategy could be a versatile tool to explain a potential and pharmacological activity of ANT and ANT-OH.https://www.mdpi.com/1422-0067/22/7/3665antazolineatrial fibrillationbiomedical applicationscore–shell nanostructuresdispersive solid phase extractionhydroxyantazoline |
| spellingShingle | Joanna Giebułtowicz Natalia Korytowska Monika Sobiech Sebastian Polak Barbara Wiśniowska Roman Piotrowski Piotr Kułakowski Piotr Luliński Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and Pharmacokinetics International Journal of Molecular Sciences antazoline atrial fibrillation biomedical applications core–shell nanostructures dispersive solid phase extraction hydroxyantazoline |
| title | Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and Pharmacokinetics |
| title_full | Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and Pharmacokinetics |
| title_fullStr | Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and Pharmacokinetics |
| title_full_unstemmed | Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and Pharmacokinetics |
| title_short | Magnetic Core–Shell Molecularly Imprinted Nano-Conjugates for Extraction of Antazoline and Hydroxyantazoline from Human Plasma—Material Characterization, Theoretical Analysis and Pharmacokinetics |
| title_sort | magnetic core shell molecularly imprinted nano conjugates for extraction of antazoline and hydroxyantazoline from human plasma material characterization theoretical analysis and pharmacokinetics |
| topic | antazoline atrial fibrillation biomedical applications core–shell nanostructures dispersive solid phase extraction hydroxyantazoline |
| url | https://www.mdpi.com/1422-0067/22/7/3665 |
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