Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus
Subarachnoid hemorrhage is a specific, life-threatening form of hemorrhagic stroke linked to high morbidity and mortality. It has been found that the choroid plexus of the brain ventricles forming the blood-cerebrospinal fluid barrier plays an important role in subarachnoid hemorrhage pathophysiolog...
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| Format: | Article |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2020.593305/full |
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| author | Peter Solár Peter Solár Peter Solár Václav Brázda Václav Brázda Shahaf Levin Alemeh Zamani Radim Jančálek Radim Jančálek Petr Dubový Marek Joukal |
| author_facet | Peter Solár Peter Solár Peter Solár Václav Brázda Václav Brázda Shahaf Levin Alemeh Zamani Radim Jančálek Radim Jančálek Petr Dubový Marek Joukal |
| author_sort | Peter Solár |
| collection | DOAJ |
| description | Subarachnoid hemorrhage is a specific, life-threatening form of hemorrhagic stroke linked to high morbidity and mortality. It has been found that the choroid plexus of the brain ventricles forming the blood-cerebrospinal fluid barrier plays an important role in subarachnoid hemorrhage pathophysiology. Heme oxygenase-1 and biliverdin reductase are two of the key enzymes of the hemoglobin degradation cascade. Therefore, the aim of present study was to investigate changes in protein levels of heme oxygenase-1 and biliverdin reductase in the rat choroid plexus after experimental subarachnoid hemorrhage induced by injection of non-heparinized autologous blood to the cisterna magna. Artificial cerebrospinal fluid of the same volume as autologous blood was injected to mimic increased intracranial pressure in control rats. Immunohistochemical and Western blot analyses were used to monitor changes in the of heme oxygenase-1 and biliverdin reductase levels in the rat choroid plexus after induction of subarachnoid hemorrhage or artificial cerebrospinal fluid application for 1, 3, and 7 days. We found increased levels of heme oxygenase-1 and biliverdin reductase protein in the choroid plexus over the entire period following subarachnoid hemorrhage induction. The level of heme oxygenase-1 was the highest early (1 and 3 days) after subarachnoid hemorrhage indicating its importance in hemoglobin degradation. Increased levels of heme oxygenase-1 were also observed in the choroid plexus epithelial cells at all time points after application of artificial cerebrospinal fluid. Biliverdin reductase protein was detected mainly in the choroid plexus epithelial cells, with levels gradually increasing during subarachnoid hemorrhage. Our results suggest that heme oxygenase-1 and biliverdin reductase are involved not only in hemoglobin degradation but probably also in protecting choroid plexus epithelial cells and the blood-cerebrospinal fluid barrier from the negative effects of subarachnoid hemorrhage. |
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| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-17T01:24:31Z |
| publishDate | 2020-11-01 |
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| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-09c1922dce144879800fd97c9f79acaa2022-12-21T22:08:44ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022020-11-011410.3389/fncel.2020.593305593305Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid PlexusPeter Solár0Peter Solár1Peter Solár2Václav Brázda3Václav Brázda4Shahaf Levin5Alemeh Zamani6Radim Jančálek7Radim Jančálek8Petr Dubový9Marek Joukal10Department of Anatomy, Faculty of Medicine, Cellular and Molecular Neurobiology Research Group, Masaryk University, Brno, CzechiaDepartment of Neurosurgery – St. Anne’s University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Neurosurgery, St. Anne’s University Hospital Brno, Brno, CzechiaDepartment of Anatomy, Faculty of Medicine, Cellular and Molecular Neurobiology Research Group, Masaryk University, Brno, CzechiaInstitute of Biophysics of the Czech Academy of Sciences, Brno, CzechiaDepartment of Anatomy, Faculty of Medicine, Cellular and Molecular Neurobiology Research Group, Masaryk University, Brno, CzechiaDepartment of Anatomy, Faculty of Medicine, Cellular and Molecular Neurobiology Research Group, Masaryk University, Brno, CzechiaDepartment of Neurosurgery – St. Anne’s University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, CzechiaDepartment of Neurosurgery, St. Anne’s University Hospital Brno, Brno, CzechiaDepartment of Anatomy, Faculty of Medicine, Cellular and Molecular Neurobiology Research Group, Masaryk University, Brno, CzechiaDepartment of Anatomy, Faculty of Medicine, Cellular and Molecular Neurobiology Research Group, Masaryk University, Brno, CzechiaSubarachnoid hemorrhage is a specific, life-threatening form of hemorrhagic stroke linked to high morbidity and mortality. It has been found that the choroid plexus of the brain ventricles forming the blood-cerebrospinal fluid barrier plays an important role in subarachnoid hemorrhage pathophysiology. Heme oxygenase-1 and biliverdin reductase are two of the key enzymes of the hemoglobin degradation cascade. Therefore, the aim of present study was to investigate changes in protein levels of heme oxygenase-1 and biliverdin reductase in the rat choroid plexus after experimental subarachnoid hemorrhage induced by injection of non-heparinized autologous blood to the cisterna magna. Artificial cerebrospinal fluid of the same volume as autologous blood was injected to mimic increased intracranial pressure in control rats. Immunohistochemical and Western blot analyses were used to monitor changes in the of heme oxygenase-1 and biliverdin reductase levels in the rat choroid plexus after induction of subarachnoid hemorrhage or artificial cerebrospinal fluid application for 1, 3, and 7 days. We found increased levels of heme oxygenase-1 and biliverdin reductase protein in the choroid plexus over the entire period following subarachnoid hemorrhage induction. The level of heme oxygenase-1 was the highest early (1 and 3 days) after subarachnoid hemorrhage indicating its importance in hemoglobin degradation. Increased levels of heme oxygenase-1 were also observed in the choroid plexus epithelial cells at all time points after application of artificial cerebrospinal fluid. Biliverdin reductase protein was detected mainly in the choroid plexus epithelial cells, with levels gradually increasing during subarachnoid hemorrhage. Our results suggest that heme oxygenase-1 and biliverdin reductase are involved not only in hemoglobin degradation but probably also in protecting choroid plexus epithelial cells and the blood-cerebrospinal fluid barrier from the negative effects of subarachnoid hemorrhage.https://www.frontiersin.org/articles/10.3389/fncel.2020.593305/fullsubarachnoid hemorrhagechoroid plexusheme oxygenase-1biliverdin reductasemacrophages |
| spellingShingle | Peter Solár Peter Solár Peter Solár Václav Brázda Václav Brázda Shahaf Levin Alemeh Zamani Radim Jančálek Radim Jančálek Petr Dubový Marek Joukal Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus Frontiers in Cellular Neuroscience subarachnoid hemorrhage choroid plexus heme oxygenase-1 biliverdin reductase macrophages |
| title | Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus |
| title_full | Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus |
| title_fullStr | Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus |
| title_full_unstemmed | Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus |
| title_short | Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus |
| title_sort | subarachnoid hemorrhage increases level of heme oxygenase 1 and biliverdin reductase in the choroid plexus |
| topic | subarachnoid hemorrhage choroid plexus heme oxygenase-1 biliverdin reductase macrophages |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2020.593305/full |
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