Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen
AIM: To investigate the role of procollagen C-proteinase enhancer 1 (PCPE1) in retinal angiogenesis and relevant mechanisms. METHODS: The Pcolce1-knockout (KO) mice were used to explore the effect of PCPE1 on retinal angiogenesis in vivo. Pcolce1 siRNA were designed, cell count kit 8 (CCK8) assays a...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2022-06-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/en_publish/2022/6/20220603.pdf |
| _version_ | 1818007481158205440 |
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| author | Jia Luo Pei-Quan Zhao Hao-Jie Chen Miao-Miao Liu Jia-Qi He Ping Fei |
| author_facet | Jia Luo Pei-Quan Zhao Hao-Jie Chen Miao-Miao Liu Jia-Qi He Ping Fei |
| author_sort | Jia Luo |
| collection | DOAJ |
| description | AIM: To investigate the role of procollagen C-proteinase enhancer 1 (PCPE1) in retinal angiogenesis and relevant mechanisms. METHODS: The Pcolce1-knockout (KO) mice were used to explore the effect of PCPE1 on retinal angiogenesis in vivo. Pcolce1 siRNA were designed, cell count kit 8 (CCK8) assays and tube formation assays were performed to investigate the cell proliferation and tube formation abilities of retinal microvascular endothelial cells (hRMECs) in vitro. Mouse embryo fibroblasts (MEF) cells were isolated and cultured to analyze the effect of PCPE1 on enhancing procollagen cleavage. RESULTS: In vivo studies showed that the retinal vascular density of Pcolce1-/- mice was significantly lower than that of the control group. Furthermore, silencing of Pcolce1 inhibited cell proliferation and tube formation abilities of hRMECs in vitro. Additionally, much more pro-collagen was found in Pcolce1-/- MEF cells, compared to wild type MEF cells. CONCLUSION: PCPE1 may promote physiological retinal angiogenesis by regulating the processing of collagen, which may provide a potential therapeutic target of retinal vascular disease. |
| first_indexed | 2024-04-14T05:16:07Z |
| format | Article |
| id | doaj.art-09c1accf530e49b5b20e4e24399ec48c |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-14T05:16:07Z |
| publishDate | 2022-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-09c1accf530e49b5b20e4e24399ec48c2022-12-22T02:10:22ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982022-06-0115686887510.18240/ijo.2022.06.0320220603Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagenJia Luo0Pei-Quan Zhao1Hao-Jie Chen2Miao-Miao Liu3Jia-Qi He4Ping Fei5Ping Fei. Department of Ophthalmology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No.1665 Kongjiang Road, Shanghai 200092, China. feiping@xinhuamed.com.cn; Jia-Qi He. Department of General Surgery, Huadong Hospital, Fudan University, No.221 West Yan'an Road, Shanghai 200040, China. jacky_he@163.comDepartment of Ophthalmology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaDepartment of Urology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaDepartment of Ophthalmology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaDepartment of General Surgery, Huadong Hospital, Fudan University, Shanghai 200040, ChinaDepartment of Ophthalmology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaAIM: To investigate the role of procollagen C-proteinase enhancer 1 (PCPE1) in retinal angiogenesis and relevant mechanisms. METHODS: The Pcolce1-knockout (KO) mice were used to explore the effect of PCPE1 on retinal angiogenesis in vivo. Pcolce1 siRNA were designed, cell count kit 8 (CCK8) assays and tube formation assays were performed to investigate the cell proliferation and tube formation abilities of retinal microvascular endothelial cells (hRMECs) in vitro. Mouse embryo fibroblasts (MEF) cells were isolated and cultured to analyze the effect of PCPE1 on enhancing procollagen cleavage. RESULTS: In vivo studies showed that the retinal vascular density of Pcolce1-/- mice was significantly lower than that of the control group. Furthermore, silencing of Pcolce1 inhibited cell proliferation and tube formation abilities of hRMECs in vitro. Additionally, much more pro-collagen was found in Pcolce1-/- MEF cells, compared to wild type MEF cells. CONCLUSION: PCPE1 may promote physiological retinal angiogenesis by regulating the processing of collagen, which may provide a potential therapeutic target of retinal vascular disease.http://ies.ijo.cn/en_publish/2022/6/20220603.pdfretinal diseasesangiogenesisprocollagen c-proteinase enhancer 1collagen |
| spellingShingle | Jia Luo Pei-Quan Zhao Hao-Jie Chen Miao-Miao Liu Jia-Qi He Ping Fei Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen International Journal of Ophthalmology retinal diseases angiogenesis procollagen c-proteinase enhancer 1 collagen |
| title | Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen |
| title_full | Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen |
| title_fullStr | Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen |
| title_full_unstemmed | Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen |
| title_short | Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen |
| title_sort | procollagen c proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen |
| topic | retinal diseases angiogenesis procollagen c-proteinase enhancer 1 collagen |
| url | http://ies.ijo.cn/en_publish/2022/6/20220603.pdf |
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