Graphene Oxide Loaded on TiO<sub>2</sub>-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts
Surface topography, protein adsorption, and the loading of coating materials can affect soft tissue sealing. Graphene oxide (GO) is a promising candidate for improving material surface functionalization to facilitate soft tissue integration between cells and biomaterials. In this study, TiO<sub&g...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8723 |
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| author | Xu Cao Keyi Wu Caiyun Wang Yatong Guo Ran Lu Xin Wang Su Chen |
| author_facet | Xu Cao Keyi Wu Caiyun Wang Yatong Guo Ran Lu Xin Wang Su Chen |
| author_sort | Xu Cao |
| collection | DOAJ |
| description | Surface topography, protein adsorption, and the loading of coating materials can affect soft tissue sealing. Graphene oxide (GO) is a promising candidate for improving material surface functionalization to facilitate soft tissue integration between cells and biomaterials. In this study, TiO<sub>2</sub> nanotubes (TNTs) were prepared by the anodization of Ti, and TNT-graphene oxide composites (TNT-GO) were prepared by subsequent electroplating. The aim of this study was to investigate the effect of TNTs and TNT-GO surface modifications on the behavior of human gingival fibroblasts (HGFs). Commercially pure Ti and TNTs were used as the control group, and the TNT-GO surface was used as the experimental group. Scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction were used to perform sample characterization. Cell adhesion, cell proliferation, cell immunofluorescence staining, a wound-healing assay, real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and Western blotting showed that the proliferation, adhesion, migration, and adhesion-related relative gene expression of HGFs on TNT-GO were significantly enhanced compared to the control groups, which may be mediated by the activation of integrin β1 and the MAPK-Erk1/2 pathway. Our findings suggest that the biological reactivity of HGFs can be enhanced by the TNT-GO surface, thereby improving the soft tissue sealing ability. |
| first_indexed | 2024-03-09T10:07:03Z |
| format | Article |
| id | doaj.art-09c3dd4f16f24f6bb69a11a6246a817b |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:07:03Z |
| publishDate | 2022-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-09c3dd4f16f24f6bb69a11a6246a817b2023-12-01T22:58:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012315872310.3390/ijms23158723Graphene Oxide Loaded on TiO<sub>2</sub>-Nanotube-Modified Ti Regulates the Behavior of Human Gingival FibroblastsXu Cao0Keyi Wu1Caiyun Wang2Yatong Guo3Ran Lu4Xin Wang5Su Chen6Laboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, ChinaLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, ChinaLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, ChinaLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, ChinaLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, ChinaLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, ChinaLaboratory of Biomaterials and Biomechanics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, ChinaSurface topography, protein adsorption, and the loading of coating materials can affect soft tissue sealing. Graphene oxide (GO) is a promising candidate for improving material surface functionalization to facilitate soft tissue integration between cells and biomaterials. In this study, TiO<sub>2</sub> nanotubes (TNTs) were prepared by the anodization of Ti, and TNT-graphene oxide composites (TNT-GO) were prepared by subsequent electroplating. The aim of this study was to investigate the effect of TNTs and TNT-GO surface modifications on the behavior of human gingival fibroblasts (HGFs). Commercially pure Ti and TNTs were used as the control group, and the TNT-GO surface was used as the experimental group. Scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction were used to perform sample characterization. Cell adhesion, cell proliferation, cell immunofluorescence staining, a wound-healing assay, real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and Western blotting showed that the proliferation, adhesion, migration, and adhesion-related relative gene expression of HGFs on TNT-GO were significantly enhanced compared to the control groups, which may be mediated by the activation of integrin β1 and the MAPK-Erk1/2 pathway. Our findings suggest that the biological reactivity of HGFs can be enhanced by the TNT-GO surface, thereby improving the soft tissue sealing ability.https://www.mdpi.com/1422-0067/23/15/8723soft tissue integrationhuman gingival fibroblaststitanium dioxide nanotubesgraphene oxideMAPK pathway |
| spellingShingle | Xu Cao Keyi Wu Caiyun Wang Yatong Guo Ran Lu Xin Wang Su Chen Graphene Oxide Loaded on TiO<sub>2</sub>-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts International Journal of Molecular Sciences soft tissue integration human gingival fibroblasts titanium dioxide nanotubes graphene oxide MAPK pathway |
| title | Graphene Oxide Loaded on TiO<sub>2</sub>-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts |
| title_full | Graphene Oxide Loaded on TiO<sub>2</sub>-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts |
| title_fullStr | Graphene Oxide Loaded on TiO<sub>2</sub>-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts |
| title_full_unstemmed | Graphene Oxide Loaded on TiO<sub>2</sub>-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts |
| title_short | Graphene Oxide Loaded on TiO<sub>2</sub>-Nanotube-Modified Ti Regulates the Behavior of Human Gingival Fibroblasts |
| title_sort | graphene oxide loaded on tio sub 2 sub nanotube modified ti regulates the behavior of human gingival fibroblasts |
| topic | soft tissue integration human gingival fibroblasts titanium dioxide nanotubes graphene oxide MAPK pathway |
| url | https://www.mdpi.com/1422-0067/23/15/8723 |
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