Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cells
Dietary sulforaphane (SF) exerts anticancer effects via epigenetic changes resulting from the inhibition of histone deacetylase (HDAC) activity in colon and prostate cancer cells. The present study aimed to evaluate the inhibition of HDAC activity by SF and its effect on apoptosis in B16 melanoma ce...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464615003606 |
| _version_ | 1819294266455752704 |
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| author | Wu Yuanfeng Xiao Gongnian Mao Jianwei Liu Shiwang Huang Jun Mei Lehe |
| author_facet | Wu Yuanfeng Xiao Gongnian Mao Jianwei Liu Shiwang Huang Jun Mei Lehe |
| author_sort | Wu Yuanfeng |
| collection | DOAJ |
| description | Dietary sulforaphane (SF) exerts anticancer effects via epigenetic changes resulting from the inhibition of histone deacetylase (HDAC) activity in colon and prostate cancer cells. The present study aimed to evaluate the inhibition of HDAC activity by SF and its effect on apoptosis in B16 melanoma cells. SF significantly inhibited proliferation of B16 melanoma cells with an IC50 of 6.1 ± 1.0 µM, as assessed with the MTT assay performed after SF treatment for 48 h. SF induced apoptosis in B16 cells in a dose-dependent manner and caused them to undergo G2/M arrest. By quantitative real-time PCR and western blot analysis, expression of p21 and Bax was upregulated in B16 cells treated for 48 h with SF, and the levels of acetylated histones H3 and H4 were increased. In conclusion, these findings suggest that SF as functional food ingredients may be effective against melanoma through inhibition of HDAC activity. |
| first_indexed | 2024-12-24T04:23:35Z |
| format | Article |
| id | doaj.art-09c63275132544559d963364ea6d3231 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T04:23:35Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-09c63275132544559d963364ea6d32312022-12-21T17:15:42ZengElsevierJournal of Functional Foods1756-46462015-10-0118182189Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cellsWu Yuanfeng0Xiao Gongnian1Mao Jianwei2Liu Shiwang3Huang Jun4Mei Lehe5School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China; Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Produces, Hangzhou 310023, Zhejiang, China; Corresponding author. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China. Tel.: +86 571 85070398; fax: +86 571 85070371.School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China; Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Produces, Hangzhou 310023, Zhejiang, ChinaSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China; Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Produces, Hangzhou 310023, Zhejiang, China; Corresponding author. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China. Tel.: +86 571 85070392; fax: +86 571 85070392.School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China; Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Produces, Hangzhou 310023, Zhejiang, ChinaSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China; Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Produces, Hangzhou 310023, Zhejiang, ChinaDepartment of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China; Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, Zhejiang, ChinaDietary sulforaphane (SF) exerts anticancer effects via epigenetic changes resulting from the inhibition of histone deacetylase (HDAC) activity in colon and prostate cancer cells. The present study aimed to evaluate the inhibition of HDAC activity by SF and its effect on apoptosis in B16 melanoma cells. SF significantly inhibited proliferation of B16 melanoma cells with an IC50 of 6.1 ± 1.0 µM, as assessed with the MTT assay performed after SF treatment for 48 h. SF induced apoptosis in B16 cells in a dose-dependent manner and caused them to undergo G2/M arrest. By quantitative real-time PCR and western blot analysis, expression of p21 and Bax was upregulated in B16 cells treated for 48 h with SF, and the levels of acetylated histones H3 and H4 were increased. In conclusion, these findings suggest that SF as functional food ingredients may be effective against melanoma through inhibition of HDAC activity.http://www.sciencedirect.com/science/article/pii/S1756464615003606SulforaphaneHistone deacetylaseMelanomaApoptosisCell cycle arrestFunctional food |
| spellingShingle | Wu Yuanfeng Xiao Gongnian Mao Jianwei Liu Shiwang Huang Jun Mei Lehe Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cells Journal of Functional Foods Sulforaphane Histone deacetylase Melanoma Apoptosis Cell cycle arrest Functional food |
| title | Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cells |
| title_full | Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cells |
| title_fullStr | Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cells |
| title_full_unstemmed | Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cells |
| title_short | Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cells |
| title_sort | dietary sulforaphane inhibits histone deacetylase activity in b16 melanoma cells |
| topic | Sulforaphane Histone deacetylase Melanoma Apoptosis Cell cycle arrest Functional food |
| url | http://www.sciencedirect.com/science/article/pii/S1756464615003606 |
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