Sustainability of Populations of Wild Rice Species in Natural in situ Conservation Sites of South and West Sulawesi
Exploration and identification of wild rice species (Oryza spp.) was carried out in South and West Sulawesi area of the Sulawesi Island in Indonesia. We collected several accessions of wild rice in these surveys and identified species of accessions through DNA analysis. Two accessions of wild rice w...
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| Format: | Article |
| Language: | English |
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Hasanuddin University
2015-12-01
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| Series: | International Journal of Agriculture System |
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| Online Access: | http://pasca.unhas.ac.id/ojs/index.php/ijas/article/view/108 |
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| author | Rinaldi Sjahril Nurhaya J. Panga Muhammad Riadi Tadashi Sato Ikuo Nakamura |
| author_facet | Rinaldi Sjahril Nurhaya J. Panga Muhammad Riadi Tadashi Sato Ikuo Nakamura |
| author_sort | Rinaldi Sjahril |
| collection | DOAJ |
| description | Exploration and identification of wild rice species (Oryza spp.) was carried out in South and West Sulawesi area of the Sulawesi Island in Indonesia. We collected several accessions of wild rice in these surveys and identified species of accessions through DNA analysis. Two accessions of wild rice were collected in Palopo (IS-03) and Malangke (IS-20) of South Sulawesi and one accession was collected in Mamuju of West Sulawesi (IS-23). Total DNA was isolated from leaves of each accession using a modified CTAB method. Then, DNA fragment containing intron 19 sequence of PolA1 gene was amplified by PCR using a pairs of primers, 19ex5P and 20ex3P. DNA sequence of the amplified PCR product was determined by direct sequencing method. The result showed that these three wild rice accessions collected in South and West Sulawesi were identified as Oryza officinalis. Sequence analysis also showed that there were two types of the intron 19 sequences in O. officinalis. Two accessions from Palopo and Mamuju had the intron 19 sequence (298 bp) that was identical to that of O. officinalis accession (W0614) from Prome in Burma. In contrast, the accession from Malangke had the sequence (283 bp) that was shared with W0002 accession from Bangkok in Thailand. The habitats of O. officinalis were swamp or a temporary flooded plain, for example at the edge of the ditch and the rivers that are flooded in the rainy season. Sustainability of populations of this wild rice species in natural in situ conservation sites is now dangerously threatened by suburbia town expansions during the last five years. |
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| format | Article |
| id | doaj.art-09c636042a9948f98292fdae911ef592 |
| institution | Directory Open Access Journal |
| issn | 2337-9782 2580-6815 |
| language | English |
| last_indexed | 2024-04-11T04:32:09Z |
| publishDate | 2015-12-01 |
| publisher | Hasanuddin University |
| record_format | Article |
| series | International Journal of Agriculture System |
| spelling | doaj.art-09c636042a9948f98292fdae911ef5922022-12-29T03:41:56ZengHasanuddin UniversityInternational Journal of Agriculture System2337-97822580-68152015-12-013220521110.20956/ijas.v3i2.10876Sustainability of Populations of Wild Rice Species in Natural in situ Conservation Sites of South and West SulawesiRinaldi Sjahril0Nurhaya J. Panga1Muhammad Riadi2Tadashi Sato3Ikuo Nakamura4Laboratory of Plant Biosciences and Reproduction Biotechnology, Department of Agronomy, Faculty of Agriculture, Hasanuddin University, MakassarGraduate Program in Agriculture Systems, Hasanuddin University, MakassarLaboratory of Plant Breeding and Seed Sciences, Department of Agronomy, Faculty of Agriculture, Hasanuddin University, MakassarLaboratory of Environmental Biotechnology, Graduate School of Agricultural Sciences, Tohoku University, Sendai, MiyagiLaboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba UniversityExploration and identification of wild rice species (Oryza spp.) was carried out in South and West Sulawesi area of the Sulawesi Island in Indonesia. We collected several accessions of wild rice in these surveys and identified species of accessions through DNA analysis. Two accessions of wild rice were collected in Palopo (IS-03) and Malangke (IS-20) of South Sulawesi and one accession was collected in Mamuju of West Sulawesi (IS-23). Total DNA was isolated from leaves of each accession using a modified CTAB method. Then, DNA fragment containing intron 19 sequence of PolA1 gene was amplified by PCR using a pairs of primers, 19ex5P and 20ex3P. DNA sequence of the amplified PCR product was determined by direct sequencing method. The result showed that these three wild rice accessions collected in South and West Sulawesi were identified as Oryza officinalis. Sequence analysis also showed that there were two types of the intron 19 sequences in O. officinalis. Two accessions from Palopo and Mamuju had the intron 19 sequence (298 bp) that was identical to that of O. officinalis accession (W0614) from Prome in Burma. In contrast, the accession from Malangke had the sequence (283 bp) that was shared with W0002 accession from Bangkok in Thailand. The habitats of O. officinalis were swamp or a temporary flooded plain, for example at the edge of the ditch and the rivers that are flooded in the rainy season. Sustainability of populations of this wild rice species in natural in situ conservation sites is now dangerously threatened by suburbia town expansions during the last five years.http://pasca.unhas.ac.id/ojs/index.php/ijas/article/view/108wild rice species, oryza, pola1, rna polymerase i largest subunit |
| spellingShingle | Rinaldi Sjahril Nurhaya J. Panga Muhammad Riadi Tadashi Sato Ikuo Nakamura Sustainability of Populations of Wild Rice Species in Natural in situ Conservation Sites of South and West Sulawesi International Journal of Agriculture System wild rice species, oryza, pola1, rna polymerase i largest subunit |
| title | Sustainability of Populations of Wild Rice Species in Natural in situ Conservation Sites of South and West Sulawesi |
| title_full | Sustainability of Populations of Wild Rice Species in Natural in situ Conservation Sites of South and West Sulawesi |
| title_fullStr | Sustainability of Populations of Wild Rice Species in Natural in situ Conservation Sites of South and West Sulawesi |
| title_full_unstemmed | Sustainability of Populations of Wild Rice Species in Natural in situ Conservation Sites of South and West Sulawesi |
| title_short | Sustainability of Populations of Wild Rice Species in Natural in situ Conservation Sites of South and West Sulawesi |
| title_sort | sustainability of populations of wild rice species in natural in situ conservation sites of south and west sulawesi |
| topic | wild rice species, oryza, pola1, rna polymerase i largest subunit |
| url | http://pasca.unhas.ac.id/ojs/index.php/ijas/article/view/108 |
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