Levels and Significance of Arachidonic Acid Metabolites in Hypertensive Disorders of Pregnancy: A Prospective Cohort Study
Background: This project aimed to investigate the role of two arachidonic acid metabolites (14,15-epoxyeicosatrienoic acid (14,15-EET) and 15-hydroxyeicosateraenoic acid (15-HETE)) as a precursor of hypertensive disorders in pregnancy by comparing their serum levels between third-trimester hypertens...
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| Format: | Article |
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IMR Press
2023-12-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/50/12/10.31083/j.ceog5012263 |
| _version_ | 1797365336376344576 |
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| author | Xueyan Cao Jiaqi Yang Yu Zhu Xiaoqing Huo Junze Bao Liuhong Shi Min Su |
| author_facet | Xueyan Cao Jiaqi Yang Yu Zhu Xiaoqing Huo Junze Bao Liuhong Shi Min Su |
| author_sort | Xueyan Cao |
| collection | DOAJ |
| description | Background: This project aimed to investigate the role of two arachidonic acid metabolites (14,15-epoxyeicosatrienoic acid (14,15-EET) and 15-hydroxyeicosateraenoic acid (15-HETE)) as a precursor of hypertensive disorders in pregnancy by comparing their serum levels between third-trimester hypertensive and normal pregnant women. The relationship between their differential levels and pregnancy outcomes was investigated to clarify the role of arachidonic acid metabolites in the occurrence and development of hypertensive disorders during pregnancy. Methods: This is a prospective cohort study and a total of 88 patients were included in the study. 17 of them were recognized as the gestational hypertension and 25 of them were considered to be the preeclampsia. 24 women were diagnosed with severe preeclampsia. The control group consisted of 22 healthy patients pregnancy course, with no disease at the present and in the history. For this purpose, the serum levels of 14,15-EET and 15-HETE of gestational hypertension, preeclampsia (PE) and normal pregnant women group were detected by enzyme-linked immunosorbent assay (ELISA) during the third trimester of pregnancy. It was followed by detecting placental cytochrome P450 2J2 (CYP2J2) and 15-lipoxygenase-2 (15-LOX-2) expression and localization using immunohistochemistry, their corresponding proteins employing western blotting. All outcomes of maternal pregnancy were then statistically analyzed. Results: The analysis indicated that the levels of 14,15-EET, and 15-HETE were significantly higher (p < 0.05) in gestational hypertension compared to control, where the same differences were observed when severe preeclampsia and preeclampsia groups were compared, which were altogether higher than gestational hypertension group patients. Similarly, the 14,15-EET and 15-HETE levels were significantly (p < 0.05) higher in the adverse pregnancy outcome than in the normal pregnancy outcome group in severe preeclampsia. The immunohistochemical analysis results revealed that the positive staining for CYP2J2 and 15-LOX-2 expression in the placenta of the preeclampsia group was significant (p < 0.05) compared to the normal pregnant control and gestational hypertension groups, with significantly higher (p < 0.05) relative CYP2J2 and 15-LOX-2 protein levels in preeclampsia compared to normal control pregnancy group. Conclusions: The study concluded that the 14,15-EET and 15-HETE might be involved in gestational hypertension pathophysiology and preeclampsia, associated with disease severity and adverse pregnancy outcomes. Moreover, CYP2J2 and 15-LOX-2 signaling expression in the placenta may be related to arachidonic acid metabolites generated in preeclampsia. |
| first_indexed | 2024-03-08T16:47:40Z |
| format | Article |
| id | doaj.art-09c6f5f02d64420caa72e80a178309a2 |
| institution | Directory Open Access Journal |
| issn | 0390-6663 |
| language | English |
| last_indexed | 2024-03-08T16:47:40Z |
| publishDate | 2023-12-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj.art-09c6f5f02d64420caa72e80a178309a22024-01-05T07:27:09ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632023-12-01501226310.31083/j.ceog5012263S0390-6663(23)02220-0Levels and Significance of Arachidonic Acid Metabolites in Hypertensive Disorders of Pregnancy: A Prospective Cohort StudyXueyan Cao0Jiaqi Yang1Yu Zhu2Xiaoqing Huo3Junze Bao4Liuhong Shi5Min Su6Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Medical School of Nantong University, 226001 Nantong, Jiangsu, ChinaDepartment of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Medical School of Nantong University, 226001 Nantong, Jiangsu, ChinaDepartment of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Medical School of Nantong University, 226001 Nantong, Jiangsu, ChinaDepartment of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Medical School of Nantong University, 226001 Nantong, Jiangsu, ChinaMedical School of Nantong University, 226001 Nantong, Jiangsu, ChinaDepartment of Obstetrics and Gynecology, the Third People's Hospital of Tongzhou, 226001 Nantong, Jiangsu, ChinaDepartment of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Medical School of Nantong University, 226001 Nantong, Jiangsu, ChinaBackground: This project aimed to investigate the role of two arachidonic acid metabolites (14,15-epoxyeicosatrienoic acid (14,15-EET) and 15-hydroxyeicosateraenoic acid (15-HETE)) as a precursor of hypertensive disorders in pregnancy by comparing their serum levels between third-trimester hypertensive and normal pregnant women. The relationship between their differential levels and pregnancy outcomes was investigated to clarify the role of arachidonic acid metabolites in the occurrence and development of hypertensive disorders during pregnancy. Methods: This is a prospective cohort study and a total of 88 patients were included in the study. 17 of them were recognized as the gestational hypertension and 25 of them were considered to be the preeclampsia. 24 women were diagnosed with severe preeclampsia. The control group consisted of 22 healthy patients pregnancy course, with no disease at the present and in the history. For this purpose, the serum levels of 14,15-EET and 15-HETE of gestational hypertension, preeclampsia (PE) and normal pregnant women group were detected by enzyme-linked immunosorbent assay (ELISA) during the third trimester of pregnancy. It was followed by detecting placental cytochrome P450 2J2 (CYP2J2) and 15-lipoxygenase-2 (15-LOX-2) expression and localization using immunohistochemistry, their corresponding proteins employing western blotting. All outcomes of maternal pregnancy were then statistically analyzed. Results: The analysis indicated that the levels of 14,15-EET, and 15-HETE were significantly higher (p < 0.05) in gestational hypertension compared to control, where the same differences were observed when severe preeclampsia and preeclampsia groups were compared, which were altogether higher than gestational hypertension group patients. Similarly, the 14,15-EET and 15-HETE levels were significantly (p < 0.05) higher in the adverse pregnancy outcome than in the normal pregnancy outcome group in severe preeclampsia. The immunohistochemical analysis results revealed that the positive staining for CYP2J2 and 15-LOX-2 expression in the placenta of the preeclampsia group was significant (p < 0.05) compared to the normal pregnant control and gestational hypertension groups, with significantly higher (p < 0.05) relative CYP2J2 and 15-LOX-2 protein levels in preeclampsia compared to normal control pregnancy group. Conclusions: The study concluded that the 14,15-EET and 15-HETE might be involved in gestational hypertension pathophysiology and preeclampsia, associated with disease severity and adverse pregnancy outcomes. Moreover, CYP2J2 and 15-LOX-2 signaling expression in the placenta may be related to arachidonic acid metabolites generated in preeclampsia.https://www.imrpress.com/journal/CEOG/50/12/10.31083/j.ceog501226314,15-eet15-hetearachidonic acid metabolitesgestational hypertensionpreeclampsia |
| spellingShingle | Xueyan Cao Jiaqi Yang Yu Zhu Xiaoqing Huo Junze Bao Liuhong Shi Min Su Levels and Significance of Arachidonic Acid Metabolites in Hypertensive Disorders of Pregnancy: A Prospective Cohort Study Clinical and Experimental Obstetrics & Gynecology 14,15-eet 15-hete arachidonic acid metabolites gestational hypertension preeclampsia |
| title | Levels and Significance of Arachidonic Acid Metabolites in Hypertensive Disorders of Pregnancy: A Prospective Cohort Study |
| title_full | Levels and Significance of Arachidonic Acid Metabolites in Hypertensive Disorders of Pregnancy: A Prospective Cohort Study |
| title_fullStr | Levels and Significance of Arachidonic Acid Metabolites in Hypertensive Disorders of Pregnancy: A Prospective Cohort Study |
| title_full_unstemmed | Levels and Significance of Arachidonic Acid Metabolites in Hypertensive Disorders of Pregnancy: A Prospective Cohort Study |
| title_short | Levels and Significance of Arachidonic Acid Metabolites in Hypertensive Disorders of Pregnancy: A Prospective Cohort Study |
| title_sort | levels and significance of arachidonic acid metabolites in hypertensive disorders of pregnancy a prospective cohort study |
| topic | 14,15-eet 15-hete arachidonic acid metabolites gestational hypertension preeclampsia |
| url | https://www.imrpress.com/journal/CEOG/50/12/10.31083/j.ceog5012263 |
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