Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model
ABSTRACT Objective: To evaluate the magnetic hyperthermia therapy in glioblastoma tumor-on-a-Chip model using a microfluidics device. Methods: The magnetic nanoparticles coated with aminosilane were used for the therapy of magnetic hyperthermia, being evaluated the specific absorption rate of the...
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2020-01-01
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| Series: | Einstein (São Paulo) |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100227&tlng=pt |
| _version_ | 1818745252825006080 |
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| author | Javier Bustamante Mamani Bruna Souto Marinho Gabriel Nery de Albuquerque Rego Mariana Penteado Nucci Fernando Alvieri Ricardo Silva dos Santos João Victor Matias Ferreira Fernando Anselmo de Oliveira Lionel Fernel Gamarra |
| author_facet | Javier Bustamante Mamani Bruna Souto Marinho Gabriel Nery de Albuquerque Rego Mariana Penteado Nucci Fernando Alvieri Ricardo Silva dos Santos João Victor Matias Ferreira Fernando Anselmo de Oliveira Lionel Fernel Gamarra |
| author_sort | Javier Bustamante Mamani |
| collection | DOAJ |
| description | ABSTRACT Objective: To evaluate the magnetic hyperthermia therapy in glioblastoma tumor-on-a-Chip model using a microfluidics device. Methods: The magnetic nanoparticles coated with aminosilane were used for the therapy of magnetic hyperthermia, being evaluated the specific absorption rate of the magnetic nanoparticles at 300 Gauss and 305kHz. A preculture of C6 cells was performed before the 3D cells culture on the chip. The process of magnetic hyperthermia on the Chip was performed after administration of 20μL of magnetic nanoparticles (10mgFe/mL) using the parameters that generated the specific absorption rate value. The efficacy of magnetic hyperthermia therapy was evaluated by using the cell viability test through the following fluorescence staining: calcein acetoxymethyl ester (492/513nm), for live cells, and ethidium homodimer-1 (526/619nm) for dead cells dyes. Results: Magnetic nanoparticles when submitted to the alternating magnetic field (300 Gauss and 305kHz) produced a mean value of the specific absorption rate of 115.4±6.0W/g. The 3D culture of C6 cells evaluated by light field microscopy imaging showed the proliferation and morphology of the cells prior to the application of magnetic hyperthermia therapy. Fluorescence images showed decreased viability of cultured cells in organ-on-a-Chip by 20% and 100% after 10 and 30 minutes of the magnetic hyperthermia therapy application respectively. Conclusion: The study showed that the therapeutic process of magnetic hyperthermia in the glioblastoma on-a-chip model was effective to produce the total cell lise after 30 minutes of therapy. |
| first_indexed | 2024-12-18T02:57:15Z |
| format | Article |
| id | doaj.art-09cb9b0e2bf9437b8f2582d26243cc5c |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-12-18T02:57:15Z |
| publishDate | 2020-01-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-09cb9b0e2bf9437b8f2582d26243cc5c2022-12-21T21:23:21ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-63852020-01-011810.31744/einstein_journal/2020ao4954Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip modelJavier Bustamante Mamanihttps://orcid.org/0000-0001-5038-0070Bruna Souto Marinhohttps://orcid.org/0000-0003-3075-2724Gabriel Nery de Albuquerque Regohttps://orcid.org/0000-0003-2011-0373Mariana Penteado Nuccihttps://orcid.org/0000-0002-1502-9215Fernando Alvierihttps://orcid.org/0000-0001-9876-978XRicardo Silva dos Santoshttps://orcid.org/0000-0001-7523-8425João Victor Matias Ferreirahttps://orcid.org/0000-0001-8370-1862Fernando Anselmo de Oliveirahttps://orcid.org/0000-0002-7226-1694Lionel Fernel Gamarrahttps://orcid.org/0000-0002-3910-0047ABSTRACT Objective: To evaluate the magnetic hyperthermia therapy in glioblastoma tumor-on-a-Chip model using a microfluidics device. Methods: The magnetic nanoparticles coated with aminosilane were used for the therapy of magnetic hyperthermia, being evaluated the specific absorption rate of the magnetic nanoparticles at 300 Gauss and 305kHz. A preculture of C6 cells was performed before the 3D cells culture on the chip. The process of magnetic hyperthermia on the Chip was performed after administration of 20μL of magnetic nanoparticles (10mgFe/mL) using the parameters that generated the specific absorption rate value. The efficacy of magnetic hyperthermia therapy was evaluated by using the cell viability test through the following fluorescence staining: calcein acetoxymethyl ester (492/513nm), for live cells, and ethidium homodimer-1 (526/619nm) for dead cells dyes. Results: Magnetic nanoparticles when submitted to the alternating magnetic field (300 Gauss and 305kHz) produced a mean value of the specific absorption rate of 115.4±6.0W/g. The 3D culture of C6 cells evaluated by light field microscopy imaging showed the proliferation and morphology of the cells prior to the application of magnetic hyperthermia therapy. Fluorescence images showed decreased viability of cultured cells in organ-on-a-Chip by 20% and 100% after 10 and 30 minutes of the magnetic hyperthermia therapy application respectively. Conclusion: The study showed that the therapeutic process of magnetic hyperthermia in the glioblastoma on-a-chip model was effective to produce the total cell lise after 30 minutes of therapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100227&tlng=ptGlioblastoma/therapyHyperthermiaNanoparticlesMicrofluidicsC6 cells |
| spellingShingle | Javier Bustamante Mamani Bruna Souto Marinho Gabriel Nery de Albuquerque Rego Mariana Penteado Nucci Fernando Alvieri Ricardo Silva dos Santos João Victor Matias Ferreira Fernando Anselmo de Oliveira Lionel Fernel Gamarra Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model Einstein (São Paulo) Glioblastoma/therapy Hyperthermia Nanoparticles Microfluidics C6 cells |
| title | Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model |
| title_full | Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model |
| title_fullStr | Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model |
| title_full_unstemmed | Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model |
| title_short | Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model |
| title_sort | magnetic hyperthermia therapy in glioblastoma tumor on a chip model |
| topic | Glioblastoma/therapy Hyperthermia Nanoparticles Microfluidics C6 cells |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082020000100227&tlng=pt |
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