Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media
Introduction: Urethral stricture is characterized by urethral lumen narrowing due to fibrosis. Urethroplasty of the urethral stricture involves excision of scar, and may be followed by reconstruction of the urethra using split-thickness skin, buccal mucosa, urethral mucosa or, more recently, tissue-...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Indian Journal of Urology |
| Subjects: | |
| Online Access: | http://www.indianjurol.com/article.asp?issn=0970-1591;year=2015;volume=31;issue=2;spage=111;epage=115;aulast=Nath |
| _version_ | 1811303234129625088 |
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| author | Nilima Nath Sumit K Saraswat Shashank Jain Sridhar Koteshwar |
| author_facet | Nilima Nath Sumit K Saraswat Shashank Jain Sridhar Koteshwar |
| author_sort | Nilima Nath |
| collection | DOAJ |
| description | Introduction: Urethral stricture is characterized by urethral lumen narrowing due to fibrosis. Urethroplasty of the urethral stricture involves excision of scar, and may be followed by reconstruction of the urethra using split-thickness skin, buccal mucosa, urethral mucosa or, more recently, tissue-engineered grafts. The stricture wound healing process after urethroplasty is known to be mediated by an interaction between keratinocyte and fibroblasts; however, the underlying mechanisms are not studied in detail yet. We investigated the influence of epithelial cell-conditioned medium (ECCM) (obtained from confluent penile skin, buccal mucosa and urethral cell cultures) on the proliferation and migration of stricture fibroblasts using an in vitro scratch assay.
Materials and Methods: ECCM was collected from confluent primary epithelial cell cultures of three different human biopsies (penile skin, buccal mucosa and urethral mucosa), whereas stricture fibroblasts were isolated from human urethral stricture biopsies. The effect of ECCM on stricture fibroblasts′ proliferation and migration into the scratch was observed using a standard in vitro scratch assay over a period of 3 days. Four experiments were performed independently using four stricture fibroblasts from four patients and ECCM was collected from 12 different patients′ primary cell cultures.
Results: ECCM from primary epithelial cells cultures obtained from penile skin, buccal mucosa and urethra inhibited stricture fibroblasts′ proliferation and migration in the in vitro scratch assay.
Conclusion: These results demonstrate the ability of ECCM to inhibit the proliferation and migration of stricture fibroblasts and present it as an effective adjunct in urethroplasty, which may influence stricture wound healing and inhibit the recurrence of stricture. |
| first_indexed | 2024-04-13T07:44:18Z |
| format | Article |
| id | doaj.art-09cc5a50f35f49f782795fe6ef54e3eb |
| institution | Directory Open Access Journal |
| issn | 0970-1591 1998-3824 |
| language | English |
| last_indexed | 2024-04-13T07:44:18Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Urology |
| spelling | doaj.art-09cc5a50f35f49f782795fe6ef54e3eb2022-12-22T02:55:45ZengWolters Kluwer Medknow PublicationsIndian Journal of Urology0970-15911998-38242015-01-0131211111510.4103/0970-1591.152809Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned mediaNilima NathSumit K SaraswatShashank JainSridhar KoteshwarIntroduction: Urethral stricture is characterized by urethral lumen narrowing due to fibrosis. Urethroplasty of the urethral stricture involves excision of scar, and may be followed by reconstruction of the urethra using split-thickness skin, buccal mucosa, urethral mucosa or, more recently, tissue-engineered grafts. The stricture wound healing process after urethroplasty is known to be mediated by an interaction between keratinocyte and fibroblasts; however, the underlying mechanisms are not studied in detail yet. We investigated the influence of epithelial cell-conditioned medium (ECCM) (obtained from confluent penile skin, buccal mucosa and urethral cell cultures) on the proliferation and migration of stricture fibroblasts using an in vitro scratch assay. Materials and Methods: ECCM was collected from confluent primary epithelial cell cultures of three different human biopsies (penile skin, buccal mucosa and urethral mucosa), whereas stricture fibroblasts were isolated from human urethral stricture biopsies. The effect of ECCM on stricture fibroblasts′ proliferation and migration into the scratch was observed using a standard in vitro scratch assay over a period of 3 days. Four experiments were performed independently using four stricture fibroblasts from four patients and ECCM was collected from 12 different patients′ primary cell cultures. Results: ECCM from primary epithelial cells cultures obtained from penile skin, buccal mucosa and urethra inhibited stricture fibroblasts′ proliferation and migration in the in vitro scratch assay. Conclusion: These results demonstrate the ability of ECCM to inhibit the proliferation and migration of stricture fibroblasts and present it as an effective adjunct in urethroplasty, which may influence stricture wound healing and inhibit the recurrence of stricture.http://www.indianjurol.com/article.asp?issn=0970-1591;year=2015;volume=31;issue=2;spage=111;epage=115;aulast=NathEpithelial cell-conditioned mediumscratch assaytissue-engineered epithelial cell grafturethral strictureurethroplastywound healing |
| spellingShingle | Nilima Nath Sumit K Saraswat Shashank Jain Sridhar Koteshwar Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media Indian Journal of Urology Epithelial cell-conditioned medium scratch assay tissue-engineered epithelial cell graft urethral stricture urethroplasty wound healing |
| title | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
| title_full | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
| title_fullStr | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
| title_full_unstemmed | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
| title_short | Inhibition of proliferation and migration of stricture fibroblasts by epithelial cell-conditioned media |
| title_sort | inhibition of proliferation and migration of stricture fibroblasts by epithelial cell conditioned media |
| topic | Epithelial cell-conditioned medium scratch assay tissue-engineered epithelial cell graft urethral stricture urethroplasty wound healing |
| url | http://www.indianjurol.com/article.asp?issn=0970-1591;year=2015;volume=31;issue=2;spage=111;epage=115;aulast=Nath |
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