Microbiomic signatures of anal fistula and putative sources of microbes
Anal fistula is a common perianal disease that typically develops from an abscess caused by in-flammation in the area. It has long been believed that intestinal microbes play a significant role in its development, considering its close relation to the intestinal environment. This work attempts to id...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1332490/full |
| _version_ | 1797351602780110848 |
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| author | Jun Yang Ling Li Wenya Su Shuqin Zhang Hai Xu Mingyu Wang Wenlong Shen |
| author_facet | Jun Yang Ling Li Wenya Su Shuqin Zhang Hai Xu Mingyu Wang Wenlong Shen |
| author_sort | Jun Yang |
| collection | DOAJ |
| description | Anal fistula is a common perianal disease that typically develops from an abscess caused by in-flammation in the area. It has long been believed that intestinal microbes play a significant role in its development, considering its close relation to the intestinal environment. This work attempts to identify the microbiomic signatures of anal fistula, and putative sources of microbes by analyzing microbiomes of 7 anal fistula-associated sites in 12 patients. This study found that microbes in anal fistulas may originate from the skin surface in addition to the intestinal tract. This finding was further validated by NMDS analysis, which also indicated that the microbial communities in the inner and outer openings of the fistula were more similar to their surrounding environments. Using MaAslin2, the characteristics of the microbiome were examined, demonstrating a higher similarity between the abundant bacteria in the anal fistula samples and those found on the skin surface. Moreover, pin-to-pair analysis conducted on all subjects consistently showed a higher abundance of skin-sourced bacteria in anal fistulas. This study identifies the microbiomic signatures of anal fistula, and provides novel insights into the origin of microorganisms in anal fistulas. |
| first_indexed | 2024-03-08T13:02:52Z |
| format | Article |
| id | doaj.art-09cd9e42267b4b5b943ca7d5188a1de1 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-08T13:02:52Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-09cd9e42267b4b5b943ca7d5188a1de12024-01-19T04:30:54ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-01-011410.3389/fcimb.2024.13324901332490Microbiomic signatures of anal fistula and putative sources of microbesJun Yang0Ling Li1Wenya Su2Shuqin Zhang3Hai Xu4Mingyu Wang5Wenlong Shen6Department of Anorectal Surgery, Qilu Hospital of Shandong University (Qingdao), Qingdao, Shandong, ChinaState Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, ChinaDepartment of Anorectal Surgery, Yinan Hospital of Traditional Chinese Medicine, Linyi, Shandong, ChinaState Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, ChinaDepartment of Anorectal Surgery, Qilu Hospital of Shandong University (Qingdao), Qingdao, Shandong, ChinaAnal fistula is a common perianal disease that typically develops from an abscess caused by in-flammation in the area. It has long been believed that intestinal microbes play a significant role in its development, considering its close relation to the intestinal environment. This work attempts to identify the microbiomic signatures of anal fistula, and putative sources of microbes by analyzing microbiomes of 7 anal fistula-associated sites in 12 patients. This study found that microbes in anal fistulas may originate from the skin surface in addition to the intestinal tract. This finding was further validated by NMDS analysis, which also indicated that the microbial communities in the inner and outer openings of the fistula were more similar to their surrounding environments. Using MaAslin2, the characteristics of the microbiome were examined, demonstrating a higher similarity between the abundant bacteria in the anal fistula samples and those found on the skin surface. Moreover, pin-to-pair analysis conducted on all subjects consistently showed a higher abundance of skin-sourced bacteria in anal fistulas. This study identifies the microbiomic signatures of anal fistula, and provides novel insights into the origin of microorganisms in anal fistulas.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1332490/fullanal fistulamicrobiomesource of microbes16S rDNA amplicon sequencingmicrobiomic signatures of anal fistula |
| spellingShingle | Jun Yang Ling Li Wenya Su Shuqin Zhang Hai Xu Mingyu Wang Wenlong Shen Microbiomic signatures of anal fistula and putative sources of microbes Frontiers in Cellular and Infection Microbiology anal fistula microbiome source of microbes 16S rDNA amplicon sequencing microbiomic signatures of anal fistula |
| title | Microbiomic signatures of anal fistula and putative sources of microbes |
| title_full | Microbiomic signatures of anal fistula and putative sources of microbes |
| title_fullStr | Microbiomic signatures of anal fistula and putative sources of microbes |
| title_full_unstemmed | Microbiomic signatures of anal fistula and putative sources of microbes |
| title_short | Microbiomic signatures of anal fistula and putative sources of microbes |
| title_sort | microbiomic signatures of anal fistula and putative sources of microbes |
| topic | anal fistula microbiome source of microbes 16S rDNA amplicon sequencing microbiomic signatures of anal fistula |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1332490/full |
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