Nisin Influence on the Antimicrobial Resistance Ability of Canine Oral Enterococci
Periodontal disease (PD) is one of the most common diseases in dogs. Although previous studies have shown the potential of the antimicrobial peptide nisin for PD control, there is no information regarding its influence in the development of antimicrobial resistance or horizontal gene transfer (HGT)....
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/12/890 |
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| author | Eva Cunha Rita Janela Margarida Costa Luís Tavares Ana Salomé Veiga Manuela Oliveira |
| author_facet | Eva Cunha Rita Janela Margarida Costa Luís Tavares Ana Salomé Veiga Manuela Oliveira |
| author_sort | Eva Cunha |
| collection | DOAJ |
| description | Periodontal disease (PD) is one of the most common diseases in dogs. Although previous studies have shown the potential of the antimicrobial peptide nisin for PD control, there is no information regarding its influence in the development of antimicrobial resistance or horizontal gene transfer (HGT). Nisin’s mutant prevention concentration (MPC) and selection window (MSW) were determined for a collection of canine oral enterococci. Isolates recovered after the determination of the MPC values were characterized for their antimicrobial profile and its nisin minimum inhibitory and bactericidal concentrations. The potential of <i>vanA</i> HGT between <i>Enterococcus faecium</i> CCGU36804 and nine clinical canine staphylococci and enterococci was evaluated. Nisin MPC values ranged from 400 to more than 600 μg/mL. In comparison with the original enterococci collection, the isolates recovered after the determination of the nisin MPC showed increased resistance towards amoxicillin/clavulanate (5%), vancomycin (5%), enrofloxacin (10%), gentamicin (10%) and imipenem (15%). The HGT of <i>vanA</i> gene was not observed. This work showed that nisin selective pressure may induce changes in the bacteria’s antimicrobial resistance profile but does not influence horizontal transfer of <i>vanA</i> gene. To our knowledge, this is the first report of nisin’s MPC and MSW determination regarding canine enterococci. |
| first_indexed | 2024-03-10T14:10:22Z |
| format | Article |
| id | doaj.art-09cef4a82961406ba966f77ef3f218c6 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T14:10:22Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-09cef4a82961406ba966f77ef3f218c62023-11-21T00:14:42ZengMDPI AGAntibiotics2079-63822020-12-0191289010.3390/antibiotics9120890Nisin Influence on the Antimicrobial Resistance Ability of Canine Oral EnterococciEva Cunha0Rita Janela1Margarida Costa2Luís Tavares3Ana Salomé Veiga4Manuela Oliveira5CIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalCIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028 Lisboa, PortugalCIISA—Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, PortugalPeriodontal disease (PD) is one of the most common diseases in dogs. Although previous studies have shown the potential of the antimicrobial peptide nisin for PD control, there is no information regarding its influence in the development of antimicrobial resistance or horizontal gene transfer (HGT). Nisin’s mutant prevention concentration (MPC) and selection window (MSW) were determined for a collection of canine oral enterococci. Isolates recovered after the determination of the MPC values were characterized for their antimicrobial profile and its nisin minimum inhibitory and bactericidal concentrations. The potential of <i>vanA</i> HGT between <i>Enterococcus faecium</i> CCGU36804 and nine clinical canine staphylococci and enterococci was evaluated. Nisin MPC values ranged from 400 to more than 600 μg/mL. In comparison with the original enterococci collection, the isolates recovered after the determination of the nisin MPC showed increased resistance towards amoxicillin/clavulanate (5%), vancomycin (5%), enrofloxacin (10%), gentamicin (10%) and imipenem (15%). The HGT of <i>vanA</i> gene was not observed. This work showed that nisin selective pressure may induce changes in the bacteria’s antimicrobial resistance profile but does not influence horizontal transfer of <i>vanA</i> gene. To our knowledge, this is the first report of nisin’s MPC and MSW determination regarding canine enterococci.https://www.mdpi.com/2079-6382/9/12/890nisinmutant prevention concentrationmutant selection windowantimicrobial susceptibility testinghorizontal gene transfer |
| spellingShingle | Eva Cunha Rita Janela Margarida Costa Luís Tavares Ana Salomé Veiga Manuela Oliveira Nisin Influence on the Antimicrobial Resistance Ability of Canine Oral Enterococci Antibiotics nisin mutant prevention concentration mutant selection window antimicrobial susceptibility testing horizontal gene transfer |
| title | Nisin Influence on the Antimicrobial Resistance Ability of Canine Oral Enterococci |
| title_full | Nisin Influence on the Antimicrobial Resistance Ability of Canine Oral Enterococci |
| title_fullStr | Nisin Influence on the Antimicrobial Resistance Ability of Canine Oral Enterococci |
| title_full_unstemmed | Nisin Influence on the Antimicrobial Resistance Ability of Canine Oral Enterococci |
| title_short | Nisin Influence on the Antimicrobial Resistance Ability of Canine Oral Enterococci |
| title_sort | nisin influence on the antimicrobial resistance ability of canine oral enterococci |
| topic | nisin mutant prevention concentration mutant selection window antimicrobial susceptibility testing horizontal gene transfer |
| url | https://www.mdpi.com/2079-6382/9/12/890 |
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