Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images
<p/> <p>We study the problem of segmenting, reconstructing, and analyzing the structure growth of thrombi (clots) in blood vessels <it>in vivo</it> based on 2-photon microscopic image data. First, we develop an algorithm for segmenting clots in 3D microscopic images based on...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2010-01-01
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| Series: | EURASIP Journal on Advances in Signal Processing |
| Online Access: | http://asp.eurasipjournals.com/content/2010/147216 |
| _version_ | 1818537962418208768 |
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| author | Xu Zhiliang Alber MarkS Kamocka MalgorzataM Rosen ElliotD Mu Jian Liu Xiaomin Chen DannyZ |
| author_facet | Xu Zhiliang Alber MarkS Kamocka MalgorzataM Rosen ElliotD Mu Jian Liu Xiaomin Chen DannyZ |
| author_sort | Xu Zhiliang |
| collection | DOAJ |
| description | <p/> <p>We study the problem of segmenting, reconstructing, and analyzing the structure growth of thrombi (clots) in blood vessels <it>in vivo</it> based on 2-photon microscopic image data. First, we develop an algorithm for segmenting clots in 3D microscopic images based on density-based clustering and methods for dealing with imaging artifacts. Next, we apply the union-of-balls (or alpha-shape) algorithm to reconstruct the boundary of clots in 3D. Finally, we perform experimental studies and analysis on the reconstructed clots and obtain quantitative data of thrombus growth and structures. We conduct experiments on laser-induced injuries in vessels of two types of mice (the wild type and the type with low levels of coagulation factor VII) and analyze and compare the developing clot structures based on their reconstructed clots from image data. The results we obtain are of biomedical significance. Our quantitative analysis of the clot composition leads to better understanding of the thrombus development, and is valuable to the modeling and verification of computational simulation of thrombogenesis.</p> |
| first_indexed | 2024-12-11T18:57:28Z |
| format | Article |
| id | doaj.art-09d0372d6c684fc88a2ff2c15cfbd156 |
| institution | Directory Open Access Journal |
| issn | 1687-6172 1687-6180 |
| language | English |
| last_indexed | 2024-12-11T18:57:28Z |
| publishDate | 2010-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | EURASIP Journal on Advances in Signal Processing |
| spelling | doaj.art-09d0372d6c684fc88a2ff2c15cfbd1562022-12-22T00:54:06ZengSpringerOpenEURASIP Journal on Advances in Signal Processing1687-61721687-61802010-01-0120101147216Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy ImagesXu ZhiliangAlber MarkSKamocka MalgorzataMRosen ElliotDMu JianLiu XiaominChen DannyZ<p/> <p>We study the problem of segmenting, reconstructing, and analyzing the structure growth of thrombi (clots) in blood vessels <it>in vivo</it> based on 2-photon microscopic image data. First, we develop an algorithm for segmenting clots in 3D microscopic images based on density-based clustering and methods for dealing with imaging artifacts. Next, we apply the union-of-balls (or alpha-shape) algorithm to reconstruct the boundary of clots in 3D. Finally, we perform experimental studies and analysis on the reconstructed clots and obtain quantitative data of thrombus growth and structures. We conduct experiments on laser-induced injuries in vessels of two types of mice (the wild type and the type with low levels of coagulation factor VII) and analyze and compare the developing clot structures based on their reconstructed clots from image data. The results we obtain are of biomedical significance. Our quantitative analysis of the clot composition leads to better understanding of the thrombus development, and is valuable to the modeling and verification of computational simulation of thrombogenesis.</p>http://asp.eurasipjournals.com/content/2010/147216 |
| spellingShingle | Xu Zhiliang Alber MarkS Kamocka MalgorzataM Rosen ElliotD Mu Jian Liu Xiaomin Chen DannyZ Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images EURASIP Journal on Advances in Signal Processing |
| title | Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images |
| title_full | Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images |
| title_fullStr | Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images |
| title_full_unstemmed | Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images |
| title_short | Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images |
| title_sort | segmentation reconstruction and analysis of blood thrombus formation in 3d 2 photon microscopy images |
| url | http://asp.eurasipjournals.com/content/2010/147216 |
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