Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet
The objectives of this study were: 1) to evaluate the effects of a fructose enriched diet (FED) on rat sperm quality, epididymal function (i.e. oxidative stress and alpha-glucosidase expression) and testosterone concentrations; 2) to determine if the administration of ghrelin (Ghrl), reverses the ef...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019365454 |
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| author | Nicolás David Ramírez Eugenia Mercedes Luque Xaviar Michael Jones Pedro Javier Torres María José Moreira Espinoza Verónica Cantarelli Marina Flavia Ponzio Ana Arja María Belén Rabaglino Ana Carolina Martini |
| author_facet | Nicolás David Ramírez Eugenia Mercedes Luque Xaviar Michael Jones Pedro Javier Torres María José Moreira Espinoza Verónica Cantarelli Marina Flavia Ponzio Ana Arja María Belén Rabaglino Ana Carolina Martini |
| author_sort | Nicolás David Ramírez |
| collection | DOAJ |
| description | The objectives of this study were: 1) to evaluate the effects of a fructose enriched diet (FED) on rat sperm quality, epididymal function (i.e. oxidative stress and alpha-glucosidase expression) and testosterone concentrations; 2) to determine if the administration of ghrelin (Ghrl), reverses the effects induced by FED.After validating the protocol as an inductor of metabolic syndrome like-symptoms, adult male rats were assigned to one of the following treatments for 8 weeks: FED = 10% fructose enriched in water (v/v); FED + Ghrl = fructose enriched diet plus Ghrl (6 nmol/animal/day, s.c.) from week 6–8; or C = water without fructose (n = 5–10 animals/group).FED significantly decreased sperm concentration and motile sperm count/ml vs C (FED: 19.0 ± 1.6 × 106sperm/ml and 834.6 ± 137.0, respectively vs C: 25.8 ± 2.8 × 106 and 1300.4 ± 202.4, respectively; p < 0.05); ghrelin injection reversed this negative effect (23.5 ± 1.6 × 106sperm/ml and 1381.7 ± 71.3 respectively). FED resulted in hypogonadism, but Ghrl could not normalize testosterone concentrations (C: 1.4 ± 0.1 ng/ml vs FED: 0.8 ± 0.2 ng/ml and FED + Ghrl: 0.6 ± 0.2 ng/ml; p < 0.05). Ghrelin did not reverse metabolic abnormalities secondary to FED. FED did not alter epididymal expression of antioxidants enzymes (superoxido-dismutase, catalase and glutathione peroxidases –Gpx-). Nevertheless, FED + Ghrl significantly increased the expression of Gpx3 (FED + Ghrl: 3.47 ± 0.48 vs FED: 0.69 ± 0.28 and C: 1.00 ± 0.14; p < 0.05). The expression of neutral alpha-glucosidase, which is a marker of epididymal function, did not differ between treatments.In conclusion, the administration of Ghrl modulated the negative effects of FED on sperm quality, possibly by an epididymal increase in Gpx3 expression. However, Ghrl could not neither normalize the metabolism of FED animals, nor reverse hypogonadism. |
| first_indexed | 2024-12-23T10:23:38Z |
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| id | doaj.art-09d2787030014b7698d5d79fbcc76212 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-23T10:23:38Z |
| publishDate | 2019-11-01 |
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| spelling | doaj.art-09d2787030014b7698d5d79fbcc762122022-12-21T17:50:37ZengElsevierHeliyon2405-84402019-11-01511e02886Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched dietNicolás David Ramírez0Eugenia Mercedes Luque1Xaviar Michael Jones2Pedro Javier Torres3María José Moreira Espinoza4Verónica Cantarelli5Marina Flavia Ponzio6Ana Arja7María Belén Rabaglino8Ana Carolina Martini9Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, ArgentinaInstituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, ArgentinaInstituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, ArgentinaInstituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, Argentina; Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET-Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enrique Barros esq, Enfermera Gordillo, Pabellón de Biología Celular, Ciudad Universitaria, 5016, Córdoba, ArgentinaInstituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enrique Barros esq, Enfermera Gordillo, Ciudad Universitaria, 5016, Córdoba, ArgentinaInstituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, Argentina; Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET-Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enrique Barros esq, Enfermera Gordillo, Pabellón de Biología Celular, Ciudad Universitaria, 5016, Córdoba, ArgentinaInstituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, Argentina; Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET-Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enrique Barros esq, Enfermera Gordillo, Pabellón de Biología Celular, Ciudad Universitaria, 5016, Córdoba, ArgentinaInstituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, ArgentinaInstituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET-Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enrique Barros esq, Enfermera Gordillo, Pabellón de Biología Celular, Ciudad Universitaria, 5016, Córdoba, ArgentinaInstituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, X5000ESU, Córdoba, Argentina; Instituto de Investigaciones en Ciencias de la Salud (INICSA), CONICET-Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Enrique Barros esq, Enfermera Gordillo, Pabellón de Biología Celular, Ciudad Universitaria, 5016, Córdoba, Argentina; Corresponding author.The objectives of this study were: 1) to evaluate the effects of a fructose enriched diet (FED) on rat sperm quality, epididymal function (i.e. oxidative stress and alpha-glucosidase expression) and testosterone concentrations; 2) to determine if the administration of ghrelin (Ghrl), reverses the effects induced by FED.After validating the protocol as an inductor of metabolic syndrome like-symptoms, adult male rats were assigned to one of the following treatments for 8 weeks: FED = 10% fructose enriched in water (v/v); FED + Ghrl = fructose enriched diet plus Ghrl (6 nmol/animal/day, s.c.) from week 6–8; or C = water without fructose (n = 5–10 animals/group).FED significantly decreased sperm concentration and motile sperm count/ml vs C (FED: 19.0 ± 1.6 × 106sperm/ml and 834.6 ± 137.0, respectively vs C: 25.8 ± 2.8 × 106 and 1300.4 ± 202.4, respectively; p < 0.05); ghrelin injection reversed this negative effect (23.5 ± 1.6 × 106sperm/ml and 1381.7 ± 71.3 respectively). FED resulted in hypogonadism, but Ghrl could not normalize testosterone concentrations (C: 1.4 ± 0.1 ng/ml vs FED: 0.8 ± 0.2 ng/ml and FED + Ghrl: 0.6 ± 0.2 ng/ml; p < 0.05). Ghrelin did not reverse metabolic abnormalities secondary to FED. FED did not alter epididymal expression of antioxidants enzymes (superoxido-dismutase, catalase and glutathione peroxidases –Gpx-). Nevertheless, FED + Ghrl significantly increased the expression of Gpx3 (FED + Ghrl: 3.47 ± 0.48 vs FED: 0.69 ± 0.28 and C: 1.00 ± 0.14; p < 0.05). The expression of neutral alpha-glucosidase, which is a marker of epididymal function, did not differ between treatments.In conclusion, the administration of Ghrl modulated the negative effects of FED on sperm quality, possibly by an epididymal increase in Gpx3 expression. However, Ghrl could not neither normalize the metabolism of FED animals, nor reverse hypogonadism.http://www.sciencedirect.com/science/article/pii/S2405844019365454Cell biologyDevelopmental biologyMolecular biologyDietEndocrinologyMetabolic syndrome |
| spellingShingle | Nicolás David Ramírez Eugenia Mercedes Luque Xaviar Michael Jones Pedro Javier Torres María José Moreira Espinoza Verónica Cantarelli Marina Flavia Ponzio Ana Arja María Belén Rabaglino Ana Carolina Martini Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet Heliyon Cell biology Developmental biology Molecular biology Diet Endocrinology Metabolic syndrome |
| title | Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet |
| title_full | Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet |
| title_fullStr | Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet |
| title_full_unstemmed | Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet |
| title_short | Modulatory effects of ghrelin on sperm quality alterations induced by a fructose-enriched diet |
| title_sort | modulatory effects of ghrelin on sperm quality alterations induced by a fructose enriched diet |
| topic | Cell biology Developmental biology Molecular biology Diet Endocrinology Metabolic syndrome |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019365454 |
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