The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens
The redox imbalanced 6 mutant (rimb6) of Arabidopsis thaliana was isolated in a genetic screening approach for mutants with defects in chloroplast-to-nucleus redox signaling. It has an atypically low activation status of the 2-Cys peroxiredoxin-A promoter in the seedling stage. rimb6 shows wildtype-...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01650/full |
| _version_ | 1819075333324800000 |
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| author | Christiane Hedtmann Wei Guo Elena Reifschneider Isabelle Heiber Heiko Hiltscher Jörn van Buer Aiko Barsch Karsten Niehaus Beth Rowan Tobias Lortzing Anke Steppuhn Margarete Baier |
| author_facet | Christiane Hedtmann Wei Guo Elena Reifschneider Isabelle Heiber Heiko Hiltscher Jörn van Buer Aiko Barsch Karsten Niehaus Beth Rowan Tobias Lortzing Anke Steppuhn Margarete Baier |
| author_sort | Christiane Hedtmann |
| collection | DOAJ |
| description | The redox imbalanced 6 mutant (rimb6) of Arabidopsis thaliana was isolated in a genetic screening approach for mutants with defects in chloroplast-to-nucleus redox signaling. It has an atypically low activation status of the 2-Cys peroxiredoxin-A promoter in the seedling stage. rimb6 shows wildtype-like germination, seedling development and greening, but slower growth and reduced biomass in the rosette stage. Mapping of the casual mutation revealed that rimb6 carries a single nucleotide polymorphism in the gene encoding CONSTITUTIVE EXPRESSER OF PATHOGENESIS RELATED (PR) GENES 1, CPR1 (At4g12560), leading to a premature stop codon. CPR1 is known as a repressor of pathogen signaling and regulator of microtubule organization. Allelism of rimb6 and cpr1 revealed a function of CPR1 in chloroplast stress protection. Expression studies in pathogen signaling mutants demonstrated that CPR1-mediated activation of genes for photosynthesis and chloroplast antioxidant protection is, in contrast to activation of pathogen responses, regulated independently from PAD4-controlled salicylic acid (SA) accumulation. We conclude that the support of plastid function is a basic, SA-independent function of CPR1. |
| first_indexed | 2024-12-21T18:23:44Z |
| format | Article |
| id | doaj.art-09d603ab16c64f4c8129d61269f66953 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T18:23:44Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-09d603ab16c64f4c8129d61269f669532022-12-21T18:54:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-09-01810.3389/fpls.2017.01650290421The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of PathogensChristiane Hedtmann0Wei Guo1Elena Reifschneider2Isabelle Heiber3Heiko Hiltscher4Jörn van Buer5Aiko Barsch6Karsten Niehaus7Beth Rowan8Tobias Lortzing9Anke Steppuhn10Margarete Baier11Plant Physiology, Dahlem Centre of Plant Sciences, Free University of BerlinBerlin, GermanyPlant Physiology, Dahlem Centre of Plant Sciences, Free University of BerlinBerlin, GermanyPlant Physiology, Dahlem Centre of Plant Sciences, Free University of BerlinBerlin, GermanyPlant Physiology and Biochemistry, Bielefeld UniversityBielefeld, GermanyPlant Sciences, Heinrich Heine University of DüsseldorfDüsseldorf, GermanyPlant Physiology, Dahlem Centre of Plant Sciences, Free University of BerlinBerlin, GermanyProteom- und Metabolomforschung, Bielefeld UniversityBielefeld, GermanyProteom- und Metabolomforschung, Bielefeld UniversityBielefeld, GermanyDepartment of Molecular Biology, Max Planck Institute for Developmental BiologyTübingen, GermanyDepartment of Molecular Ecology, Free University of BerlinBerlin, GermanyDepartment of Molecular Ecology, Free University of BerlinBerlin, GermanyPlant Physiology, Dahlem Centre of Plant Sciences, Free University of BerlinBerlin, GermanyThe redox imbalanced 6 mutant (rimb6) of Arabidopsis thaliana was isolated in a genetic screening approach for mutants with defects in chloroplast-to-nucleus redox signaling. It has an atypically low activation status of the 2-Cys peroxiredoxin-A promoter in the seedling stage. rimb6 shows wildtype-like germination, seedling development and greening, but slower growth and reduced biomass in the rosette stage. Mapping of the casual mutation revealed that rimb6 carries a single nucleotide polymorphism in the gene encoding CONSTITUTIVE EXPRESSER OF PATHOGENESIS RELATED (PR) GENES 1, CPR1 (At4g12560), leading to a premature stop codon. CPR1 is known as a repressor of pathogen signaling and regulator of microtubule organization. Allelism of rimb6 and cpr1 revealed a function of CPR1 in chloroplast stress protection. Expression studies in pathogen signaling mutants demonstrated that CPR1-mediated activation of genes for photosynthesis and chloroplast antioxidant protection is, in contrast to activation of pathogen responses, regulated independently from PAD4-controlled salicylic acid (SA) accumulation. We conclude that the support of plastid function is a basic, SA-independent function of CPR1.http://journal.frontiersin.org/article/10.3389/fpls.2017.01650/fullArabidopsischloroplastCPR1reactive oxygen speciesredox imbalanced mutantsignaling |
| spellingShingle | Christiane Hedtmann Wei Guo Elena Reifschneider Isabelle Heiber Heiko Hiltscher Jörn van Buer Aiko Barsch Karsten Niehaus Beth Rowan Tobias Lortzing Anke Steppuhn Margarete Baier The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens Frontiers in Plant Science Arabidopsis chloroplast CPR1 reactive oxygen species redox imbalanced mutant signaling |
| title | The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens |
| title_full | The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens |
| title_fullStr | The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens |
| title_full_unstemmed | The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens |
| title_short | The Plant Immunity Regulating F-Box Protein CPR1 Supports Plastid Function in Absence of Pathogens |
| title_sort | plant immunity regulating f box protein cpr1 supports plastid function in absence of pathogens |
| topic | Arabidopsis chloroplast CPR1 reactive oxygen species redox imbalanced mutant signaling |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01650/full |
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