Assessment of different methods for the detection of biofilm production in coagulase-negative staphylococci isolated from blood cultures of newborns
Abstract INTRODUCTION: Coagulase-negative staphylococci (CoNS) are a frequent cause of bacteremia, especially in neonates. The major virulence determinant in CoNS is the ability to produce biofilms, which is conferred by the icaADBC genes. This study aimed to assess different methods for the detect...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
|
| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000600761&lng=en&tlng=en |
| _version_ | 1818263865757007872 |
|---|---|
| author | Roberta Filipini Rampelotto Vinicius Victor Lorenzoni Danielly da Costa Silva Silvana Silveira Coelho Vanessa Wust Litiérri Razia Garzon Melise Silveira Nunes Bettina Meneghetti Patrícia Chaves Brites Manfredo Hörner Rosmari Hörner |
| author_facet | Roberta Filipini Rampelotto Vinicius Victor Lorenzoni Danielly da Costa Silva Silvana Silveira Coelho Vanessa Wust Litiérri Razia Garzon Melise Silveira Nunes Bettina Meneghetti Patrícia Chaves Brites Manfredo Hörner Rosmari Hörner |
| author_sort | Roberta Filipini Rampelotto |
| collection | DOAJ |
| description | Abstract INTRODUCTION: Coagulase-negative staphylococci (CoNS) are a frequent cause of bacteremia, especially in neonates. The major virulence determinant in CoNS is the ability to produce biofilms, which is conferred by the icaADBC genes. This study aimed to assess different methods for the detection of biofilm formation in 176 CoNS isolates from blood cultures of newborns. METHODS: The presence of the icaACD genes was assessed by polymerase chain reaction (PCR), and biofilm formation was assessed on congo red agar (CRA), by the tube method (TM), and on tissue culture plates (TCP). RESULTS: Of the 176 CoNS isolates, 30.1% expressed icaACD and 11.4% expressed icaAD. The CRA assay and TM showed that 42% and 38.6% of the isolates were biofilm producing, respectively. On TCP, 40.9% of the isolates produced biofilms; 21% were weakly adherent and 19.9% were strongly adherent. When compared to the gold standard technique (PCR), the CRAassay showed 79% sensitivity and 84% specificity (kappa = 0.64), TM showed 78% sensitivity and 89% specificity (kappa = 0.68), and TCP showed 99% sensitivity and 100% specificity (kappa = 0.99). CONCLUSIONS: In this study, ~42% of CoNS isolates produced biofilms, and the presence of icaACD was associated with a greater capacity to form biofilms. Compared to the other phenotypic methodologies, TCP is an ideal procedure for routine laboratory use. |
| first_indexed | 2024-12-12T19:25:49Z |
| format | Article |
| id | doaj.art-09da245bfc484e48a73594e33ce4a768 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T19:25:49Z |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-09da245bfc484e48a73594e33ce4a7682022-12-22T00:14:31ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-984951676176710.1590/0037-8682-0171-2018S0037-86822018000600761Assessment of different methods for the detection of biofilm production in coagulase-negative staphylococci isolated from blood cultures of newbornsRoberta Filipini RampelottoVinicius Victor LorenzoniDanielly da Costa SilvaSilvana Silveira CoelhoVanessa WustLitiérri Razia GarzonMelise Silveira NunesBettina MeneghettiPatrícia Chaves BritesManfredo HörnerRosmari HörnerAbstract INTRODUCTION: Coagulase-negative staphylococci (CoNS) are a frequent cause of bacteremia, especially in neonates. The major virulence determinant in CoNS is the ability to produce biofilms, which is conferred by the icaADBC genes. This study aimed to assess different methods for the detection of biofilm formation in 176 CoNS isolates from blood cultures of newborns. METHODS: The presence of the icaACD genes was assessed by polymerase chain reaction (PCR), and biofilm formation was assessed on congo red agar (CRA), by the tube method (TM), and on tissue culture plates (TCP). RESULTS: Of the 176 CoNS isolates, 30.1% expressed icaACD and 11.4% expressed icaAD. The CRA assay and TM showed that 42% and 38.6% of the isolates were biofilm producing, respectively. On TCP, 40.9% of the isolates produced biofilms; 21% were weakly adherent and 19.9% were strongly adherent. When compared to the gold standard technique (PCR), the CRAassay showed 79% sensitivity and 84% specificity (kappa = 0.64), TM showed 78% sensitivity and 89% specificity (kappa = 0.68), and TCP showed 99% sensitivity and 100% specificity (kappa = 0.99). CONCLUSIONS: In this study, ~42% of CoNS isolates produced biofilms, and the presence of icaACD was associated with a greater capacity to form biofilms. Compared to the other phenotypic methodologies, TCP is an ideal procedure for routine laboratory use.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000600761&lng=en&tlng=enBiofilmBlood cultureCoagulase-negative staphylococciNewborn |
| spellingShingle | Roberta Filipini Rampelotto Vinicius Victor Lorenzoni Danielly da Costa Silva Silvana Silveira Coelho Vanessa Wust Litiérri Razia Garzon Melise Silveira Nunes Bettina Meneghetti Patrícia Chaves Brites Manfredo Hörner Rosmari Hörner Assessment of different methods for the detection of biofilm production in coagulase-negative staphylococci isolated from blood cultures of newborns Revista da Sociedade Brasileira de Medicina Tropical Biofilm Blood culture Coagulase-negative staphylococci Newborn |
| title | Assessment of different methods for the detection of biofilm production in coagulase-negative staphylococci isolated from blood cultures of newborns |
| title_full | Assessment of different methods for the detection of biofilm production in coagulase-negative staphylococci isolated from blood cultures of newborns |
| title_fullStr | Assessment of different methods for the detection of biofilm production in coagulase-negative staphylococci isolated from blood cultures of newborns |
| title_full_unstemmed | Assessment of different methods for the detection of biofilm production in coagulase-negative staphylococci isolated from blood cultures of newborns |
| title_short | Assessment of different methods for the detection of biofilm production in coagulase-negative staphylococci isolated from blood cultures of newborns |
| title_sort | assessment of different methods for the detection of biofilm production in coagulase negative staphylococci isolated from blood cultures of newborns |
| topic | Biofilm Blood culture Coagulase-negative staphylococci Newborn |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000600761&lng=en&tlng=en |
| work_keys_str_mv | AT robertafilipinirampelotto assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT viniciusvictorlorenzoni assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT daniellydacostasilva assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT silvanasilveiracoelho assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT vanessawust assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT litierriraziagarzon assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT melisesilveiranunes assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT bettinameneghetti assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT patriciachavesbrites assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT manfredohorner assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns AT rosmarihorner assessmentofdifferentmethodsforthedetectionofbiofilmproductionincoagulasenegativestaphylococciisolatedfrombloodculturesofnewborns |