LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathway
This study was designed to investigate the biological functions of LINC00482 in prostate cancer (PCa) with bone metastasis. TCGA dataset of PCa was applied for LINC00482 expression analysis and real time PCR was used to verify the expression level of LINC00482 in PCa tissues as well as PCa bone meta...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
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| Series: | Journal of Bone Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212137423000271 |
| _version_ | 1797745946842365952 |
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| author | Shiyao Liao Xuemei Fang Kai Zhou Tingxiao Zhao Lichen Ji Wei Zhang Xugang Zhong Fabo Feng Jun Lv Yao Kang Danjie Zhu |
| author_facet | Shiyao Liao Xuemei Fang Kai Zhou Tingxiao Zhao Lichen Ji Wei Zhang Xugang Zhong Fabo Feng Jun Lv Yao Kang Danjie Zhu |
| author_sort | Shiyao Liao |
| collection | DOAJ |
| description | This study was designed to investigate the biological functions of LINC00482 in prostate cancer (PCa) with bone metastasis. TCGA dataset of PCa was applied for LINC00482 expression analysis and real time PCR was used to verify the expression level of LINC00482 in PCa tissues as well as PCa bone metastatic tissues. To detect the biological functions of LINC00482 in vitro, various assays were used including CCK-8, EdU, colony formation and transwell assays. The biological functions of LINC00482 were also identified in vivo by inoculating PCa cells into the left cardiac ventricle of mice, followed by evaluating the osteolytic lesions and osteolytic score. In addition, Starbase and Lncbase databases were applied for predicting the potential target miRNA of LINC00482, while TargetScan and Starbase databases were used for predicting the potential target of miRNA. The luciferase reporter assay was utilized to determine the interactions among these molecules and western blotting was employed to verified the targeted proteins. Results showed that high expression level of LINC00482 was observed in bone metastatic PCa tissues and associated with PCa progression. Silencing of LINC00482 inhibited cell proliferation, migration and invasion in PCa. Furthermore, LINC00482 was proved to act as a competing endogenous RNA by sponging miR-2467-3p to activate Wnt/β-catenin signaling pathway, which may be a promising therapeutic target for PCa with bone metastasis. |
| first_indexed | 2024-03-12T15:31:07Z |
| format | Article |
| id | doaj.art-09db257d5b60400aa0e8d22bc9760ebb |
| institution | Directory Open Access Journal |
| issn | 2212-1374 |
| language | English |
| last_indexed | 2024-03-12T15:31:07Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Bone Oncology |
| spelling | doaj.art-09db257d5b60400aa0e8d22bc9760ebb2023-08-10T04:34:15ZengElsevierJournal of Bone Oncology2212-13742023-08-0141100494LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathwayShiyao Liao0Xuemei Fang1Kai Zhou2Tingxiao Zhao3Lichen Ji4Wei Zhang5Xugang Zhong6Fabo Feng7Jun Lv8Yao Kang9Danjie Zhu10Department of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; Hangzhou Medical College People’s Hospital, Hangzhou, Zhejiang, ChinaDepartment of Head and Neck Surgery, Sun Yat-sen University Cancer Center, Guangzhou, China; State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, ChinaThe First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang Street, Ouhai District, Wenzhou City, Zhejiang, ChinaDepartment of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; Hangzhou Medical College People’s Hospital, Hangzhou, Zhejiang, ChinaDepartment of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, ChinaDepartment of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; Qingdao University, Qingdao, ChinaDepartment of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; Qingdao University, Qingdao, ChinaDepartment of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; Hangzhou Medical College People’s Hospital, Hangzhou, Zhejiang, ChinaDepartment of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; Hangzhou Medical College People’s Hospital, Hangzhou, Zhejiang, China; Corresponding authors.Department of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; Hangzhou Medical College People’s Hospital, Hangzhou, Zhejiang, China; Corresponding authors.Department of Orthopedics, Zhejiang Provincial People’s Hospital, Shangtang Road 158#, Hangzhou, Zhejiang, China; Hangzhou Medical College People’s Hospital, Hangzhou, Zhejiang, China; Corresponding authors.This study was designed to investigate the biological functions of LINC00482 in prostate cancer (PCa) with bone metastasis. TCGA dataset of PCa was applied for LINC00482 expression analysis and real time PCR was used to verify the expression level of LINC00482 in PCa tissues as well as PCa bone metastatic tissues. To detect the biological functions of LINC00482 in vitro, various assays were used including CCK-8, EdU, colony formation and transwell assays. The biological functions of LINC00482 were also identified in vivo by inoculating PCa cells into the left cardiac ventricle of mice, followed by evaluating the osteolytic lesions and osteolytic score. In addition, Starbase and Lncbase databases were applied for predicting the potential target miRNA of LINC00482, while TargetScan and Starbase databases were used for predicting the potential target of miRNA. The luciferase reporter assay was utilized to determine the interactions among these molecules and western blotting was employed to verified the targeted proteins. Results showed that high expression level of LINC00482 was observed in bone metastatic PCa tissues and associated with PCa progression. Silencing of LINC00482 inhibited cell proliferation, migration and invasion in PCa. Furthermore, LINC00482 was proved to act as a competing endogenous RNA by sponging miR-2467-3p to activate Wnt/β-catenin signaling pathway, which may be a promising therapeutic target for PCa with bone metastasis.http://www.sciencedirect.com/science/article/pii/S2212137423000271LINC00482miR-2467-3pWnt/β-cateninProstate cancerBone metastasis |
| spellingShingle | Shiyao Liao Xuemei Fang Kai Zhou Tingxiao Zhao Lichen Ji Wei Zhang Xugang Zhong Fabo Feng Jun Lv Yao Kang Danjie Zhu LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathway Journal of Bone Oncology LINC00482 miR-2467-3p Wnt/β-catenin Prostate cancer Bone metastasis |
| title | LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathway |
| title_full | LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathway |
| title_fullStr | LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathway |
| title_full_unstemmed | LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathway |
| title_short | LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathway |
| title_sort | linc00482 sponged mir 2467 3p to promote bone metastasis of prostate cancer through activating wnt β catenin signaling pathway |
| topic | LINC00482 miR-2467-3p Wnt/β-catenin Prostate cancer Bone metastasis |
| url | http://www.sciencedirect.com/science/article/pii/S2212137423000271 |
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