Opposing Roles of FACT for Euchromatin and Heterochromatin in Yeast
DNA is stored in the nucleus of a cell in a folded state; however, only the necessary genetic information is extracted from the required group of genes. The key to extracting genetic information is chromatin ambivalence. Depending on the chromosomal region, chromatin is characterized into low-densit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/2/377 |
| _version_ | 1797622038324576256 |
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| author | Shinya Takahata Yota Murakami |
| author_facet | Shinya Takahata Yota Murakami |
| author_sort | Shinya Takahata |
| collection | DOAJ |
| description | DNA is stored in the nucleus of a cell in a folded state; however, only the necessary genetic information is extracted from the required group of genes. The key to extracting genetic information is chromatin ambivalence. Depending on the chromosomal region, chromatin is characterized into low-density “euchromatin” and high-density “heterochromatin”, with various factors being involved in its regulation. Here, we focus on chromatin regulation and gene expression by the yeast FACT complex, which functions in both euchromatin and heterochromatin. FACT is known as a histone H2A/H2B chaperone and was initially reported as an elongation factor associated with RNA polymerase II. In budding yeast, FACT activates promoter chromatin by interacting with the transcriptional activators SBF/MBF via the regulation of G1/S cell cycle genes. In fission yeast, FACT plays an important role in the formation of higher-order chromatin structures and transcriptional repression by binding to Swi6, an HP1 family protein, at heterochromatin. This FACT property, which refers to the alternate chromatin-regulation depending on the binding partner, is an interesting phenomenon. Further analysis of nucleosome regulation within heterochromatin is expected in future studies. |
| first_indexed | 2024-03-11T09:05:36Z |
| format | Article |
| id | doaj.art-09dbdcc63bd148169e08f456ed5ecbb9 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T09:05:36Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-09dbdcc63bd148169e08f456ed5ecbb92023-11-16T19:24:21ZengMDPI AGBiomolecules2218-273X2023-02-0113237710.3390/biom13020377Opposing Roles of FACT for Euchromatin and Heterochromatin in YeastShinya Takahata0Yota Murakami1Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, JapanDepartment of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, JapanDNA is stored in the nucleus of a cell in a folded state; however, only the necessary genetic information is extracted from the required group of genes. The key to extracting genetic information is chromatin ambivalence. Depending on the chromosomal region, chromatin is characterized into low-density “euchromatin” and high-density “heterochromatin”, with various factors being involved in its regulation. Here, we focus on chromatin regulation and gene expression by the yeast FACT complex, which functions in both euchromatin and heterochromatin. FACT is known as a histone H2A/H2B chaperone and was initially reported as an elongation factor associated with RNA polymerase II. In budding yeast, FACT activates promoter chromatin by interacting with the transcriptional activators SBF/MBF via the regulation of G1/S cell cycle genes. In fission yeast, FACT plays an important role in the formation of higher-order chromatin structures and transcriptional repression by binding to Swi6, an HP1 family protein, at heterochromatin. This FACT property, which refers to the alternate chromatin-regulation depending on the binding partner, is an interesting phenomenon. Further analysis of nucleosome regulation within heterochromatin is expected in future studies.https://www.mdpi.com/2218-273X/13/2/377FACTSpt16Pob3Nhp6SBFheterochromatin |
| spellingShingle | Shinya Takahata Yota Murakami Opposing Roles of FACT for Euchromatin and Heterochromatin in Yeast Biomolecules FACT Spt16 Pob3 Nhp6 SBF heterochromatin |
| title | Opposing Roles of FACT for Euchromatin and Heterochromatin in Yeast |
| title_full | Opposing Roles of FACT for Euchromatin and Heterochromatin in Yeast |
| title_fullStr | Opposing Roles of FACT for Euchromatin and Heterochromatin in Yeast |
| title_full_unstemmed | Opposing Roles of FACT for Euchromatin and Heterochromatin in Yeast |
| title_short | Opposing Roles of FACT for Euchromatin and Heterochromatin in Yeast |
| title_sort | opposing roles of fact for euchromatin and heterochromatin in yeast |
| topic | FACT Spt16 Pob3 Nhp6 SBF heterochromatin |
| url | https://www.mdpi.com/2218-273X/13/2/377 |
| work_keys_str_mv | AT shinyatakahata opposingrolesoffactforeuchromatinandheterochromatininyeast AT yotamurakami opposingrolesoffactforeuchromatinandheterochromatininyeast |