Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae)
Drosophila melanogaster Meigen, 1830 has served as a model insect for over a century. Sequencing of the 11 additional Drosophila Fallen, 1823 species marks substantial progress in comparative genomics of this genus. By comparison, practically nothing is known about the genome size or genome sequence...
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| Format: | Article |
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Pensoft Publishers
2011-08-01
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| Series: | Comparative Cytogenetics |
| Online Access: | http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1723 |
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| author | Vladimir Gokhman Spencer Johnston Chiyedza Small Roma Rajwani Shawn Hanrahan Shubha Govind |
| author_facet | Vladimir Gokhman Spencer Johnston Chiyedza Small Roma Rajwani Shawn Hanrahan Shubha Govind |
| author_sort | Vladimir Gokhman |
| collection | DOAJ |
| description | Drosophila melanogaster Meigen, 1830 has served as a model insect for over a century. Sequencing of the 11 additional Drosophila Fallen, 1823 species marks substantial progress in comparative genomics of this genus. By comparison, practically nothing is known about the genome size or genome sequences of parasitic wasps of Drosophila. Here, we present the first comparative analysis of genome size and karyotype structures of Drosophila parasitoids of the Leptopilina Förster, 1869 and Ganaspis Förster, 1869 species. The gametic genome size of Ganaspis xanthopoda (Ashmead, 1896) is larger than those of the three Leptopilina species studied. The genome sizes of all parasitic wasps studied here are also larger than those known for all Drosophila species. Surprisingly, genome sizes of these Drosophila parasitoids exceed the average value known for all previously studied Hymenoptera. The haploid chromosome number of both Leptopilina heterotoma (Thomson, 1862) and L. victoriae Nordlander, 1980 is ten. A chromosomal fusion appears to have produced a distinct karyotype for L. boulardi (Barbotin, Carton et Keiner-Pillault, 1979) (n = 9), whose genome size is smaller than that of wasps of the L. heterotoma clade. Like L. boulardi, the haploid chromosome number for G. xanthopoda is also nine. Our studies reveal a positive, but non linear, correlation between the genome size and total chromosome length in Drosophila parasitoids. These Drosophila parasitoids differ widely in their host range, and utilize different infection strategies to overcome host defense. Their comparative genomics, in relation to their exceptionally well-characterized hosts, will prove to be valuable for understanding the molecular basis of the host-parasite arms race and how such mechanisms shape the genetic structures of insect communities. |
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| institution | Directory Open Access Journal |
| issn | 1993-0771 1993-078X |
| language | English |
| last_indexed | 2024-12-19T13:29:53Z |
| publishDate | 2011-08-01 |
| publisher | Pensoft Publishers |
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| series | Comparative Cytogenetics |
| spelling | doaj.art-09dc2d0fd6b54fb1834526a976948e2c2022-12-21T20:19:26ZengPensoft PublishersComparative Cytogenetics1993-07711993-078X2011-08-015321122110.3897/compcytogen.v5i3.14351723Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae)Vladimir GokhmanSpencer JohnstonChiyedza SmallRoma RajwaniShawn HanrahanShubha GovindDrosophila melanogaster Meigen, 1830 has served as a model insect for over a century. Sequencing of the 11 additional Drosophila Fallen, 1823 species marks substantial progress in comparative genomics of this genus. By comparison, practically nothing is known about the genome size or genome sequences of parasitic wasps of Drosophila. Here, we present the first comparative analysis of genome size and karyotype structures of Drosophila parasitoids of the Leptopilina Förster, 1869 and Ganaspis Förster, 1869 species. The gametic genome size of Ganaspis xanthopoda (Ashmead, 1896) is larger than those of the three Leptopilina species studied. The genome sizes of all parasitic wasps studied here are also larger than those known for all Drosophila species. Surprisingly, genome sizes of these Drosophila parasitoids exceed the average value known for all previously studied Hymenoptera. The haploid chromosome number of both Leptopilina heterotoma (Thomson, 1862) and L. victoriae Nordlander, 1980 is ten. A chromosomal fusion appears to have produced a distinct karyotype for L. boulardi (Barbotin, Carton et Keiner-Pillault, 1979) (n = 9), whose genome size is smaller than that of wasps of the L. heterotoma clade. Like L. boulardi, the haploid chromosome number for G. xanthopoda is also nine. Our studies reveal a positive, but non linear, correlation between the genome size and total chromosome length in Drosophila parasitoids. These Drosophila parasitoids differ widely in their host range, and utilize different infection strategies to overcome host defense. Their comparative genomics, in relation to their exceptionally well-characterized hosts, will prove to be valuable for understanding the molecular basis of the host-parasite arms race and how such mechanisms shape the genetic structures of insect communities.http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1723 |
| spellingShingle | Vladimir Gokhman Spencer Johnston Chiyedza Small Roma Rajwani Shawn Hanrahan Shubha Govind Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae) Comparative Cytogenetics |
| title | Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae) |
| title_full | Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae) |
| title_fullStr | Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae) |
| title_full_unstemmed | Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae) |
| title_short | Genomic and karyotypic variation in Drosophila parasitoids (Hymenoptera, Cynipoidea, Figitidae) |
| title_sort | genomic and karyotypic variation in drosophila parasitoids hymenoptera cynipoidea figitidae |
| url | http://compcytogen.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=1723 |
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