Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacao
Abstract Background Moniliophthora perniciosa is a phytopathogenic fungus responsible for witches’ broom disease of cacao trees (Theobroma cacao L.). Understanding the molecular events during germination of the pathogen may enable the development of strategies for disease control in these economical...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-017-1085-4 |
| _version_ | 1819145902409908224 |
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| author | Joise Hander Mares Karina Peres Gramacho Everton Cruz Santos André da Silva Santiago Juliano Oliveira Santana Aurizângela Oliveira de Sousa Fátima Cerqueira Alvim Carlos Priminho Pirovani |
| author_facet | Joise Hander Mares Karina Peres Gramacho Everton Cruz Santos André da Silva Santiago Juliano Oliveira Santana Aurizângela Oliveira de Sousa Fátima Cerqueira Alvim Carlos Priminho Pirovani |
| author_sort | Joise Hander Mares |
| collection | DOAJ |
| description | Abstract Background Moniliophthora perniciosa is a phytopathogenic fungus responsible for witches’ broom disease of cacao trees (Theobroma cacao L.). Understanding the molecular events during germination of the pathogen may enable the development of strategies for disease control in these economically important plants. In this study, we determined a comparative proteomic profile of M. perniciosa basidiospores during germination by two-dimensional SDS-PAGE and mass spectrometry. Results A total of 316 proteins were identified. Molecular changes during the development of the germinative tube were identified by a hierarchical clustering analysis based on the differential accumulation of proteins. Proteins associated with fungal filamentation, such as septin and kinesin, were detected only 4 h after germination (hag). A transcription factor related to biosynthesis of the secondary metabolite fumagillin, which can form hybrids with polyketides, was induced 2 hag, and polyketide synthase was observed 4 hag. The accumulation of ATP synthase, binding immunoglobulin protein (BiP), and catalase was validated by western blotting. Conclusions In this study, we showed variations in protein expression during the early germination stages of fungus M. perniciosa. Proteins associated with fungal filamentation, and consequently with virulence, were detected in basidiospores 4 hag., for example, septin and kinesin. We discuss these results and propose a model of the germination of fungus M. perniciosa. This research can help elucidate the mechanisms underlying basic processes of host invasion and to develop strategies for control of the disease. |
| first_indexed | 2024-12-22T13:05:24Z |
| format | Article |
| id | doaj.art-09df5e192b8d4b02847d38c1e618aa4a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-22T13:05:24Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-09df5e192b8d4b02847d38c1e618aa4a2022-12-21T18:24:53ZengBMCBMC Microbiology1471-21802017-08-0117111010.1186/s12866-017-1085-4Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacaoJoise Hander Mares0Karina Peres Gramacho1Everton Cruz Santos2André da Silva Santiago3Juliano Oliveira Santana4Aurizângela Oliveira de Sousa5Fátima Cerqueira Alvim6Carlos Priminho Pirovani7Laboratory of Proteomics, Center of Biotechnology and Genetics, State University of Santa Cruz (UESC)Laboratory of Plant Pathology, Cacao Research Center, CEPLACLaboratory of Proteomics, Center of Biotechnology and Genetics, State University of Santa Cruz (UESC)Center for Molecular Biology and Genetic Engineering (CBMEG) UnicampLaboratory of Proteomics, Center of Biotechnology and Genetics, State University of Santa Cruz (UESC)Laboratory of Proteomics, Center of Biotechnology and Genetics, State University of Santa Cruz (UESC)Laboratory of Proteomics, Center of Biotechnology and Genetics, State University of Santa Cruz (UESC)Laboratory of Proteomics, Center of Biotechnology and Genetics, State University of Santa Cruz (UESC)Abstract Background Moniliophthora perniciosa is a phytopathogenic fungus responsible for witches’ broom disease of cacao trees (Theobroma cacao L.). Understanding the molecular events during germination of the pathogen may enable the development of strategies for disease control in these economically important plants. In this study, we determined a comparative proteomic profile of M. perniciosa basidiospores during germination by two-dimensional SDS-PAGE and mass spectrometry. Results A total of 316 proteins were identified. Molecular changes during the development of the germinative tube were identified by a hierarchical clustering analysis based on the differential accumulation of proteins. Proteins associated with fungal filamentation, such as septin and kinesin, were detected only 4 h after germination (hag). A transcription factor related to biosynthesis of the secondary metabolite fumagillin, which can form hybrids with polyketides, was induced 2 hag, and polyketide synthase was observed 4 hag. The accumulation of ATP synthase, binding immunoglobulin protein (BiP), and catalase was validated by western blotting. Conclusions In this study, we showed variations in protein expression during the early germination stages of fungus M. perniciosa. Proteins associated with fungal filamentation, and consequently with virulence, were detected in basidiospores 4 hag., for example, septin and kinesin. We discuss these results and propose a model of the germination of fungus M. perniciosa. This research can help elucidate the mechanisms underlying basic processes of host invasion and to develop strategies for control of the disease.http://link.springer.com/article/10.1186/s12866-017-1085-4BasidiosporeGerminationWitches’ broomProteomic analysis |
| spellingShingle | Joise Hander Mares Karina Peres Gramacho Everton Cruz Santos André da Silva Santiago Juliano Oliveira Santana Aurizângela Oliveira de Sousa Fátima Cerqueira Alvim Carlos Priminho Pirovani Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacao BMC Microbiology Basidiospore Germination Witches’ broom Proteomic analysis |
| title | Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacao |
| title_full | Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacao |
| title_fullStr | Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacao |
| title_full_unstemmed | Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacao |
| title_short | Proteomic analysis during of spore germination of Moniliophthora perniciosa, the causal agent of witches’ broom disease in cacao |
| title_sort | proteomic analysis during of spore germination of moniliophthora perniciosa the causal agent of witches broom disease in cacao |
| topic | Basidiospore Germination Witches’ broom Proteomic analysis |
| url | http://link.springer.com/article/10.1186/s12866-017-1085-4 |
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