An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in food
Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food tes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01227/full |
| _version_ | 1819096958642421760 |
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| author | Jodi Woan-Fei Law Nurul-Syakima eAb Mutalib Kok Gan eChan Learn-Han eLee |
| author_facet | Jodi Woan-Fei Law Nurul-Syakima eAb Mutalib Kok Gan eChan Learn-Han eLee |
| author_sort | Jodi Woan-Fei Law |
| collection | DOAJ |
| description | Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration and molecular identification of L. monocytogenes in various food samples. There are a number of enrichment and plating media that can be used for the isolation of L. monocytogenes from food samples. Enrichment media such as buffered Listeria Enrichment Broth (BLEB), Fraser broth and University of Vermont Medium (UVM) Listeria enrichment broth are recommended by regulatory agencies such as FDA-BAM, USDA-FSIS and ISO. Many plating media are available for the isolation of L. monocytogenes, for instance, PALCAM, Oxford and other chromogenic media. Besides, reference methods like FDA-BAM, ISO 11290 method and USDA-FSIS method are usually applied for the cultural detection or enumeration of L. monocytogenes. MPN technique is applied for the enumeration of L. monocytogenes in the case of low level contamination. Molecular methods including polymerase chain reaction (PCR), multiplex polymerase chain reaction (mPCR), real-time/quantitative polymerase chain reaction (qPCR), nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), DNA microarray and Next Generation Sequencing (NGS) technology for the detection and identification of L. monocytogenes are discussed in this review. Overall, molecular methods are rapid, sensitive, specific, time- and labour-saving. In future, there are chances for the development of new techniques for the detection and identification of foodborne with improved features. |
| first_indexed | 2024-12-22T00:07:28Z |
| format | Article |
| id | doaj.art-09e3494d2fc44f1b9dbc110f142586af |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T00:07:28Z |
| publishDate | 2015-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-09e3494d2fc44f1b9dbc110f142586af2022-12-21T18:45:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-11-01610.3389/fmicb.2015.01227163566An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in foodJodi Woan-Fei Law0Nurul-Syakima eAb Mutalib1Kok Gan eChan2Learn-Han eLee3Monash University MalaysiaUKM Medical Molecular Biology Institute (UMBI)University of MalayaMonash University MalaysiaListeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration and molecular identification of L. monocytogenes in various food samples. There are a number of enrichment and plating media that can be used for the isolation of L. monocytogenes from food samples. Enrichment media such as buffered Listeria Enrichment Broth (BLEB), Fraser broth and University of Vermont Medium (UVM) Listeria enrichment broth are recommended by regulatory agencies such as FDA-BAM, USDA-FSIS and ISO. Many plating media are available for the isolation of L. monocytogenes, for instance, PALCAM, Oxford and other chromogenic media. Besides, reference methods like FDA-BAM, ISO 11290 method and USDA-FSIS method are usually applied for the cultural detection or enumeration of L. monocytogenes. MPN technique is applied for the enumeration of L. monocytogenes in the case of low level contamination. Molecular methods including polymerase chain reaction (PCR), multiplex polymerase chain reaction (mPCR), real-time/quantitative polymerase chain reaction (qPCR), nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP), DNA microarray and Next Generation Sequencing (NGS) technology for the detection and identification of L. monocytogenes are discussed in this review. Overall, molecular methods are rapid, sensitive, specific, time- and labour-saving. In future, there are chances for the development of new techniques for the detection and identification of foodborne with improved features.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01227/fullListeria monocytogenesfoodborne pathogensEnumerationIsolationmolecular detection |
| spellingShingle | Jodi Woan-Fei Law Nurul-Syakima eAb Mutalib Kok Gan eChan Learn-Han eLee An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in food Frontiers in Microbiology Listeria monocytogenes foodborne pathogens Enumeration Isolation molecular detection |
| title | An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in food |
| title_full | An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in food |
| title_fullStr | An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in food |
| title_full_unstemmed | An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in food |
| title_short | An insight into the isolation, enumeration and molecular detection of Listeria monocytogenes in food |
| title_sort | insight into the isolation enumeration and molecular detection of listeria monocytogenes in food |
| topic | Listeria monocytogenes foodborne pathogens Enumeration Isolation molecular detection |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01227/full |
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