Endothelial nitric oxide synthase gene Glu298Asp polymorphism in patients with coronary artery disease
<b>Background and objectives</b> : Endo-derived nitric oxide (NO) is synthesized from L-arginine by endothelial nitric oxide synthase (NOS3). Since reduced NO synthesis in endothelial cells has been implicated in the development of coronary atherosclerosis, we investigated the associatio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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King Faisal Specialist Hospital and Research Centre
2010-01-01
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| Series: | Annals of Saudi Medicine |
| Online Access: | http://www.saudiannals.net/article.asp?issn=0256-4947;year=2010;volume=30;issue=1;spage=33;epage=37;aulast=Salimi |
| _version_ | 1811299013353275392 |
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| author | Salimi Saeedeh Firoozrai Mohsen Zand Hamid Nakhaee Alireza Shafiee Sayed Tavilani Heidar Mohebbi Ahmad |
| author_facet | Salimi Saeedeh Firoozrai Mohsen Zand Hamid Nakhaee Alireza Shafiee Sayed Tavilani Heidar Mohebbi Ahmad |
| author_sort | Salimi Saeedeh |
| collection | DOAJ |
| description | <b>Background and objectives</b> : Endo-derived nitric oxide (NO) is synthesized from L-arginine by endothelial nitric oxide synthase (NOS3). Since reduced NO synthesis in endothelial cells has been implicated in the development of coronary atherosclerosis, we investigated the association of NOS3 gene polymorphisms and coronary artery disease (CAD) in an Iranian population. <b> Subjects and Methods</b> : We studied the NOS3 gene Glu298Asp polymorphism in 241 CAD patients with positive coronary angiograms (i.e.,> 50% stenosis affecting at least one coronary vessel) in Shahid Rajaee Heart Hospital and 261 control subjects without a history of symptomatic CAD. The NOS3 gene polymorphism was analyzed by polymerase chain reaction and restriction fragment length polymorphism. Lipid profile and other risk factors were also determined. <b> Results</b> : The genotype frequencies of Glu298Asp polymorphism for Glu/Glu, Glu/Asp, and Asp/Asp were 61.3%, 32.2%, and 6.5%, respectively, in control subjects, and 46.5%, 42.7%, and 10.8% in CAD patients, respectively. The genotype frequencies differed significantly between the two groups (<i>P</i>=.003). The frequencies of the Asp alleles were 32.2% and 22.6% for CAD patients and control subjects, respectively; the difference between the two groups was statistically significant (<i>P</i>=.001; odds ratio=1.6). Plasma lipids, except HDL-C, were also significantly increased in the CAD groups. <b>Conclusion</b> : These results suggest that CAD is associated with Glu298Asp polymorphism of the NOS3 gene in our population and that this polymorphism is an independent risk factor for CAD. |
| first_indexed | 2024-04-13T06:28:52Z |
| format | Article |
| id | doaj.art-09e7245d9b844c5aac1230e275206e4e |
| institution | Directory Open Access Journal |
| issn | 0256-4947 0975-4466 |
| language | English |
| last_indexed | 2024-04-13T06:28:52Z |
| publishDate | 2010-01-01 |
| publisher | King Faisal Specialist Hospital and Research Centre |
| record_format | Article |
| series | Annals of Saudi Medicine |
| spelling | doaj.art-09e7245d9b844c5aac1230e275206e4e2022-12-22T02:58:14ZengKing Faisal Specialist Hospital and Research CentreAnnals of Saudi Medicine0256-49470975-44662010-01-013013337Endothelial nitric oxide synthase gene Glu298Asp polymorphism in patients with coronary artery diseaseSalimi SaeedehFiroozrai MohsenZand HamidNakhaee AlirezaShafiee SayedTavilani HeidarMohebbi Ahmad<b>Background and objectives</b> : Endo-derived nitric oxide (NO) is synthesized from L-arginine by endothelial nitric oxide synthase (NOS3). Since reduced NO synthesis in endothelial cells has been implicated in the development of coronary atherosclerosis, we investigated the association of NOS3 gene polymorphisms and coronary artery disease (CAD) in an Iranian population. <b> Subjects and Methods</b> : We studied the NOS3 gene Glu298Asp polymorphism in 241 CAD patients with positive coronary angiograms (i.e.,> 50% stenosis affecting at least one coronary vessel) in Shahid Rajaee Heart Hospital and 261 control subjects without a history of symptomatic CAD. The NOS3 gene polymorphism was analyzed by polymerase chain reaction and restriction fragment length polymorphism. Lipid profile and other risk factors were also determined. <b> Results</b> : The genotype frequencies of Glu298Asp polymorphism for Glu/Glu, Glu/Asp, and Asp/Asp were 61.3%, 32.2%, and 6.5%, respectively, in control subjects, and 46.5%, 42.7%, and 10.8% in CAD patients, respectively. The genotype frequencies differed significantly between the two groups (<i>P</i>=.003). The frequencies of the Asp alleles were 32.2% and 22.6% for CAD patients and control subjects, respectively; the difference between the two groups was statistically significant (<i>P</i>=.001; odds ratio=1.6). Plasma lipids, except HDL-C, were also significantly increased in the CAD groups. <b>Conclusion</b> : These results suggest that CAD is associated with Glu298Asp polymorphism of the NOS3 gene in our population and that this polymorphism is an independent risk factor for CAD.http://www.saudiannals.net/article.asp?issn=0256-4947;year=2010;volume=30;issue=1;spage=33;epage=37;aulast=Salimi |
| spellingShingle | Salimi Saeedeh Firoozrai Mohsen Zand Hamid Nakhaee Alireza Shafiee Sayed Tavilani Heidar Mohebbi Ahmad Endothelial nitric oxide synthase gene Glu298Asp polymorphism in patients with coronary artery disease Annals of Saudi Medicine |
| title | Endothelial nitric oxide synthase gene Glu298Asp polymorphism in patients with coronary artery disease |
| title_full | Endothelial nitric oxide synthase gene Glu298Asp polymorphism in patients with coronary artery disease |
| title_fullStr | Endothelial nitric oxide synthase gene Glu298Asp polymorphism in patients with coronary artery disease |
| title_full_unstemmed | Endothelial nitric oxide synthase gene Glu298Asp polymorphism in patients with coronary artery disease |
| title_short | Endothelial nitric oxide synthase gene Glu298Asp polymorphism in patients with coronary artery disease |
| title_sort | endothelial nitric oxide synthase gene glu298asp polymorphism in patients with coronary artery disease |
| url | http://www.saudiannals.net/article.asp?issn=0256-4947;year=2010;volume=30;issue=1;spage=33;epage=37;aulast=Salimi |
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