Characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis B and C in respect to severity of morphological changes of the liver
Aim. To study cytokine concentration in blood serum in spontaneous and NDV- and PGA stimulated production by peripheral blood mononuclear cells (PBMC) and contents of peripheral blood lymphocyte subpopulations when compared with severity of morphological changes of the liver in patients with chronic...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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"Consilium Medicum" Publishing house
2009-04-01
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| Series: | Терапевтический архив |
| Subjects: | |
| Online Access: | https://ter-arkhiv.ru/0040-3660/article/view/30317 |
| _version_ | 1828417201560879104 |
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| author | A S Lazareva E V Volchkova K T Umbetova V P Chulanov Yu G Parkhomenko M V Kiselevskiy S G Pak A S Lazareva E V Volchkova K T Umbetova V P Chulanov Yu G Parkhomenko M V Kiselevsky S G Pak |
| author_facet | A S Lazareva E V Volchkova K T Umbetova V P Chulanov Yu G Parkhomenko M V Kiselevskiy S G Pak A S Lazareva E V Volchkova K T Umbetova V P Chulanov Yu G Parkhomenko M V Kiselevsky S G Pak |
| author_sort | A S Lazareva |
| collection | DOAJ |
| description | Aim. To study cytokine concentration in blood serum in spontaneous and NDV- and PGA stimulated production by peripheral blood mononuclear cells (PBMC) and contents of peripheral blood lymphocyte subpopulations when compared with severity of morphological changes of the liver in patients with chronic viral hepatitis B and C (CHB and CHC).
Material and methods. A total of 37 patients entered the trial (13 with CHB and 24 with CHC). Concentrations of IFN-alpha, IFN-gamma, TNF-alpha, IL-1beta, IL-4, IL-6 in blood serum in spontaneous and NDV and PGA stimulated PBMC production, content of CD3, CD4, CD8, CD16, CD25, HLADR, CD20, CD38 subpopulations of peripheral blood lymphocytes were examined. Also, puncture biopsy of the liver by Mengini was made with subsequent estimation of the histological activity index by R. Knodell adapted to hepatitis B and C by K. Ishak.
Results. Patients with CHB and CHC in minimal activity of the process and insignificant morphological changes in the liver demonstrated a significant fall of NDV induced IFN-alpha production. Patients with highly active hepatitis and maximal morphological changes in the liver had significant lowering of NDV induced production of IFN-alpha, IFN-gamma, TNF-alpha, IL-6 and PGA induced production of TNF-alpha.
Conclusion. CHB and CHC patients should be examined not only with estimation of serum cytokines concentration but also NDV and PGA stimulated production of PBMC should be taken into consideration with calculation of immunoregulatory index for prognosis of the disease and choice of adequate therapy. |
| first_indexed | 2024-12-10T14:16:16Z |
| format | Article |
| id | doaj.art-09e768f4c00a47d98989ca88862da1b3 |
| institution | Directory Open Access Journal |
| issn | 0040-3660 2309-5342 |
| language | Russian |
| last_indexed | 2024-12-10T14:16:16Z |
| publishDate | 2009-04-01 |
| publisher | "Consilium Medicum" Publishing house |
| record_format | Article |
| series | Терапевтический архив |
| spelling | doaj.art-09e768f4c00a47d98989ca88862da1b32022-12-22T01:45:19Zrus"Consilium Medicum" Publishing houseТерапевтический архив0040-36602309-53422009-04-01814555927344Characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis B and C in respect to severity of morphological changes of the liverA S LazarevaE V VolchkovaK T UmbetovaV P ChulanovYu G ParkhomenkoM V KiselevskiyS G PakA S LazarevaE V VolchkovaK T UmbetovaV P ChulanovYu G ParkhomenkoM V KiselevskyS G PakAim. To study cytokine concentration in blood serum in spontaneous and NDV- and PGA stimulated production by peripheral blood mononuclear cells (PBMC) and contents of peripheral blood lymphocyte subpopulations when compared with severity of morphological changes of the liver in patients with chronic viral hepatitis B and C (CHB and CHC). Material and methods. A total of 37 patients entered the trial (13 with CHB and 24 with CHC). Concentrations of IFN-alpha, IFN-gamma, TNF-alpha, IL-1beta, IL-4, IL-6 in blood serum in spontaneous and NDV and PGA stimulated PBMC production, content of CD3, CD4, CD8, CD16, CD25, HLADR, CD20, CD38 subpopulations of peripheral blood lymphocytes were examined. Also, puncture biopsy of the liver by Mengini was made with subsequent estimation of the histological activity index by R. Knodell adapted to hepatitis B and C by K. Ishak. Results. Patients with CHB and CHC in minimal activity of the process and insignificant morphological changes in the liver demonstrated a significant fall of NDV induced IFN-alpha production. Patients with highly active hepatitis and maximal morphological changes in the liver had significant lowering of NDV induced production of IFN-alpha, IFN-gamma, TNF-alpha, IL-6 and PGA induced production of TNF-alpha. Conclusion. CHB and CHC patients should be examined not only with estimation of serum cytokines concentration but also NDV and PGA stimulated production of PBMC should be taken into consideration with calculation of immunoregulatory index for prognosis of the disease and choice of adequate therapy.https://ter-arkhiv.ru/0040-3660/article/view/30317chronic viral hepatitis b and cindex of histological activitystimulated cytokine productionimmune status |
| spellingShingle | A S Lazareva E V Volchkova K T Umbetova V P Chulanov Yu G Parkhomenko M V Kiselevskiy S G Pak A S Lazareva E V Volchkova K T Umbetova V P Chulanov Yu G Parkhomenko M V Kiselevsky S G Pak Characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis B and C in respect to severity of morphological changes of the liver Терапевтический архив chronic viral hepatitis b and c index of histological activity stimulated cytokine production immune status |
| title | Characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis B and C in respect to severity of morphological changes of the liver |
| title_full | Characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis B and C in respect to severity of morphological changes of the liver |
| title_fullStr | Characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis B and C in respect to severity of morphological changes of the liver |
| title_full_unstemmed | Characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis B and C in respect to severity of morphological changes of the liver |
| title_short | Characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis B and C in respect to severity of morphological changes of the liver |
| title_sort | characteristics of the cytokine profile and peripheral blood lymphocyte subpopulations in patients with chronic viral hepatitis b and c in respect to severity of morphological changes of the liver |
| topic | chronic viral hepatitis b and c index of histological activity stimulated cytokine production immune status |
| url | https://ter-arkhiv.ru/0040-3660/article/view/30317 |
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