Establishing a prognostic model based on immune-related genes and identification of BIRC5 as a potential biomarker for lung adenocarcinoma patients
Abstract Background Lung adenocarcinoma (LUAD) is an extraordinarily malignant tumor, with rapidly increasing morbidity and poor prognosis. Immunotherapy has emerged as a hopeful therapeutic modality for lung adenocarcinoma. Furthermore, a prognostic model (based on immune genes) can fulfill the pur...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-023-11249-8 |
| _version_ | 1797452283933360128 |
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| author | Qianhe Ren Qifan Li Chenye Shao Pengpeng Zhang Zhuangzhuang Hu Jun Li Wei Wang Yue Yu |
| author_facet | Qianhe Ren Qifan Li Chenye Shao Pengpeng Zhang Zhuangzhuang Hu Jun Li Wei Wang Yue Yu |
| author_sort | Qianhe Ren |
| collection | DOAJ |
| description | Abstract Background Lung adenocarcinoma (LUAD) is an extraordinarily malignant tumor, with rapidly increasing morbidity and poor prognosis. Immunotherapy has emerged as a hopeful therapeutic modality for lung adenocarcinoma. Furthermore, a prognostic model (based on immune genes) can fulfill the purpose of early diagnosis and accurate prognostic prediction. Methods Immune-related mRNAs (IRmRNAs) were utilized to construct a prognostic model that sorted patients into high- and low-risk groups. Then, the prediction efficacy of our model was evaluated using a nomogram. The differences in overall survival (OS), the tumor mutation landscape, and the tumor microenvironment were further explored between different risk groups. In addition, the immune genes comprising the prognostic model were subjected to single-cell RNA sequencing to investigate the expression of these immune genes in different cells. Finally, the functions of BIRC5 were validated through in vitro experiments. Results Patients in different risk groups exhibited sharply significant variations in OS, pathway activity, immune cell infiltration, mutation patterns, and immune response. Single-cell RNA sequencing revealed that the expression level of BIRC5 was significantly high in T cells. Cell experiments further revealed that BIRC5 knockdown markedly reduced LUAD cell proliferation. Conclusion This model can function as an instrumental variable in the prognostic, molecular, and therapeutic prediction of LUAD, shedding new light on the optimal clinical practice guidelines for LUAD patients. |
| first_indexed | 2024-03-09T15:05:33Z |
| format | Article |
| id | doaj.art-09ec90bf678a465f9531139c745122ec |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-09T15:05:33Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-09ec90bf678a465f9531139c745122ec2023-11-26T13:37:37ZengBMCBMC Cancer1471-24072023-09-0123112010.1186/s12885-023-11249-8Establishing a prognostic model based on immune-related genes and identification of BIRC5 as a potential biomarker for lung adenocarcinoma patientsQianhe Ren0Qifan Li1Chenye Shao2Pengpeng Zhang3Zhuangzhuang Hu4Jun Li5Wei Wang6Yue Yu7Department of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Soochow UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Soochow UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical UniversityDepartment of Urology, Shuyang First People’s HospitalDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical UniversityDepartment of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical UniversityAbstract Background Lung adenocarcinoma (LUAD) is an extraordinarily malignant tumor, with rapidly increasing morbidity and poor prognosis. Immunotherapy has emerged as a hopeful therapeutic modality for lung adenocarcinoma. Furthermore, a prognostic model (based on immune genes) can fulfill the purpose of early diagnosis and accurate prognostic prediction. Methods Immune-related mRNAs (IRmRNAs) were utilized to construct a prognostic model that sorted patients into high- and low-risk groups. Then, the prediction efficacy of our model was evaluated using a nomogram. The differences in overall survival (OS), the tumor mutation landscape, and the tumor microenvironment were further explored between different risk groups. In addition, the immune genes comprising the prognostic model were subjected to single-cell RNA sequencing to investigate the expression of these immune genes in different cells. Finally, the functions of BIRC5 were validated through in vitro experiments. Results Patients in different risk groups exhibited sharply significant variations in OS, pathway activity, immune cell infiltration, mutation patterns, and immune response. Single-cell RNA sequencing revealed that the expression level of BIRC5 was significantly high in T cells. Cell experiments further revealed that BIRC5 knockdown markedly reduced LUAD cell proliferation. Conclusion This model can function as an instrumental variable in the prognostic, molecular, and therapeutic prediction of LUAD, shedding new light on the optimal clinical practice guidelines for LUAD patients.https://doi.org/10.1186/s12885-023-11249-8lung adenocarcinomaImmune genesRisk signaturePrognosisTumor mutation burdenImmunotherapy |
| spellingShingle | Qianhe Ren Qifan Li Chenye Shao Pengpeng Zhang Zhuangzhuang Hu Jun Li Wei Wang Yue Yu Establishing a prognostic model based on immune-related genes and identification of BIRC5 as a potential biomarker for lung adenocarcinoma patients BMC Cancer lung adenocarcinoma Immune genes Risk signature Prognosis Tumor mutation burden Immunotherapy |
| title | Establishing a prognostic model based on immune-related genes and identification of BIRC5 as a potential biomarker for lung adenocarcinoma patients |
| title_full | Establishing a prognostic model based on immune-related genes and identification of BIRC5 as a potential biomarker for lung adenocarcinoma patients |
| title_fullStr | Establishing a prognostic model based on immune-related genes and identification of BIRC5 as a potential biomarker for lung adenocarcinoma patients |
| title_full_unstemmed | Establishing a prognostic model based on immune-related genes and identification of BIRC5 as a potential biomarker for lung adenocarcinoma patients |
| title_short | Establishing a prognostic model based on immune-related genes and identification of BIRC5 as a potential biomarker for lung adenocarcinoma patients |
| title_sort | establishing a prognostic model based on immune related genes and identification of birc5 as a potential biomarker for lung adenocarcinoma patients |
| topic | lung adenocarcinoma Immune genes Risk signature Prognosis Tumor mutation burden Immunotherapy |
| url | https://doi.org/10.1186/s12885-023-11249-8 |
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