Cynomolgus macaque as an animal model for severe acute respiratory syndrome.
The emergence of severe acute respiratory syndrome (SARS) in 2002 and 2003 affected global health and caused major economic disruption. Adequate animal models are required to study the underlying pathogenesis of SARS-associated coronavirus (SARS-CoV) infection and to develop effective vaccines and t...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2006-05-01
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| Series: | PLoS Medicine |
| Online Access: | http://europepmc.org/articles/PMC1435788?pdf=render |
| _version_ | 1819009705466396672 |
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| author | James V Lawler Timothy P Endy Lisa E Hensley Aura Garrison Elizabeth A Fritz May Lesar Ralph S Baric David A Kulesh David A Norwood Leonard P Wasieloski Melanie P Ulrich Tom R Slezak Elizabeth Vitalis John W Huggins Peter B Jahrling Jason Paragas |
| author_facet | James V Lawler Timothy P Endy Lisa E Hensley Aura Garrison Elizabeth A Fritz May Lesar Ralph S Baric David A Kulesh David A Norwood Leonard P Wasieloski Melanie P Ulrich Tom R Slezak Elizabeth Vitalis John W Huggins Peter B Jahrling Jason Paragas |
| author_sort | James V Lawler |
| collection | DOAJ |
| description | The emergence of severe acute respiratory syndrome (SARS) in 2002 and 2003 affected global health and caused major economic disruption. Adequate animal models are required to study the underlying pathogenesis of SARS-associated coronavirus (SARS-CoV) infection and to develop effective vaccines and therapeutics. We report the first findings of measurable clinical disease in nonhuman primates (NHPs) infected with SARS-CoV.In order to characterize clinically relevant parameters of SARS-CoV infection in NHPs, we infected cynomolgus macaques with SARS-CoV in three groups: Group I was infected in the nares and bronchus, group II in the nares and conjunctiva, and group III intravenously. Nonhuman primates in groups I and II developed mild to moderate symptomatic illness. All NHPs demonstrated evidence of viral replication and developed neutralizing antibodies. Chest radiographs from several animals in groups I and II revealed unifocal or multifocal pneumonia that peaked between days 8 and 10 postinfection. Clinical laboratory tests were not significantly changed. Overall, inoculation by a mucosal route produced more prominent disease than did intravenous inoculation. Half of the group I animals were infected with a recombinant infectious clone SARS-CoV derived from the SARS-CoV Urbani strain. This infectious clone produced disease indistinguishable from wild-type Urbani strain.SARS-CoV infection of cynomolgus macaques did not reproduce the severe illness seen in the majority of adult human cases of SARS; however, our results suggest similarities to the milder syndrome of SARS-CoV infection characteristically seen in young children. |
| first_indexed | 2024-12-21T01:00:37Z |
| format | Article |
| id | doaj.art-09f0c2a62c5246ed8f496a27737d02b1 |
| institution | Directory Open Access Journal |
| issn | 1549-1277 1549-1676 |
| language | English |
| last_indexed | 2024-12-21T01:00:37Z |
| publishDate | 2006-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Medicine |
| spelling | doaj.art-09f0c2a62c5246ed8f496a27737d02b12022-12-21T19:21:11ZengPublic Library of Science (PLoS)PLoS Medicine1549-12771549-16762006-05-0135e14910.1371/journal.pmed.0030149Cynomolgus macaque as an animal model for severe acute respiratory syndrome.James V LawlerTimothy P EndyLisa E HensleyAura GarrisonElizabeth A FritzMay LesarRalph S BaricDavid A KuleshDavid A NorwoodLeonard P WasieloskiMelanie P UlrichTom R SlezakElizabeth VitalisJohn W HugginsPeter B JahrlingJason ParagasThe emergence of severe acute respiratory syndrome (SARS) in 2002 and 2003 affected global health and caused major economic disruption. Adequate animal models are required to study the underlying pathogenesis of SARS-associated coronavirus (SARS-CoV) infection and to develop effective vaccines and therapeutics. We report the first findings of measurable clinical disease in nonhuman primates (NHPs) infected with SARS-CoV.In order to characterize clinically relevant parameters of SARS-CoV infection in NHPs, we infected cynomolgus macaques with SARS-CoV in three groups: Group I was infected in the nares and bronchus, group II in the nares and conjunctiva, and group III intravenously. Nonhuman primates in groups I and II developed mild to moderate symptomatic illness. All NHPs demonstrated evidence of viral replication and developed neutralizing antibodies. Chest radiographs from several animals in groups I and II revealed unifocal or multifocal pneumonia that peaked between days 8 and 10 postinfection. Clinical laboratory tests were not significantly changed. Overall, inoculation by a mucosal route produced more prominent disease than did intravenous inoculation. Half of the group I animals were infected with a recombinant infectious clone SARS-CoV derived from the SARS-CoV Urbani strain. This infectious clone produced disease indistinguishable from wild-type Urbani strain.SARS-CoV infection of cynomolgus macaques did not reproduce the severe illness seen in the majority of adult human cases of SARS; however, our results suggest similarities to the milder syndrome of SARS-CoV infection characteristically seen in young children.http://europepmc.org/articles/PMC1435788?pdf=render |
| spellingShingle | James V Lawler Timothy P Endy Lisa E Hensley Aura Garrison Elizabeth A Fritz May Lesar Ralph S Baric David A Kulesh David A Norwood Leonard P Wasieloski Melanie P Ulrich Tom R Slezak Elizabeth Vitalis John W Huggins Peter B Jahrling Jason Paragas Cynomolgus macaque as an animal model for severe acute respiratory syndrome. PLoS Medicine |
| title | Cynomolgus macaque as an animal model for severe acute respiratory syndrome. |
| title_full | Cynomolgus macaque as an animal model for severe acute respiratory syndrome. |
| title_fullStr | Cynomolgus macaque as an animal model for severe acute respiratory syndrome. |
| title_full_unstemmed | Cynomolgus macaque as an animal model for severe acute respiratory syndrome. |
| title_short | Cynomolgus macaque as an animal model for severe acute respiratory syndrome. |
| title_sort | cynomolgus macaque as an animal model for severe acute respiratory syndrome |
| url | http://europepmc.org/articles/PMC1435788?pdf=render |
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