CRISPR/Cas9-mediated genome editing in nonhuman primates
Owing to their high similarity to humans, non-human primates (NHPs) provide an exceedingly suitable model for the study of human disease. In this Review, we summarize the history of transgenic NHP models and the progress of CRISPR/Cas9-mediated genome editing in NHPs, from the first proof-of-princip...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2019-10-01
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| Series: | Disease Models & Mechanisms |
| Subjects: | |
| Online Access: | http://dmm.biologists.org/content/12/10/dmm039982 |
| _version_ | 1818920298476470272 |
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| author | Yu Kang Chu Chu Fang Wang Yuyu Niu |
| author_facet | Yu Kang Chu Chu Fang Wang Yuyu Niu |
| author_sort | Yu Kang |
| collection | DOAJ |
| description | Owing to their high similarity to humans, non-human primates (NHPs) provide an exceedingly suitable model for the study of human disease. In this Review, we summarize the history of transgenic NHP models and the progress of CRISPR/Cas9-mediated genome editing in NHPs, from the first proof-of-principle green fluorescent protein-expressing monkeys to sophisticated NHP models of human neurodegenerative disease that accurately phenocopy several complex disease features. We discuss not only the breakthroughs and advantages, but also the potential shortcomings of the application of the CRISPR/Cas9 system to NHPs that have emerged from the expanded understanding of this technology in recent years. Although off-target and mosaic mutations are the main concerns in CRISPR/Cas9-mediated NHP modeling, recent progress in genome editing techniques make it likely that these technical limitations will be overcome soon, bringing excellent prospects to human disease studies. |
| first_indexed | 2024-12-20T01:19:32Z |
| format | Article |
| id | doaj.art-09f0f4dcd0da4fc7a999ccb3adeb1ce0 |
| institution | Directory Open Access Journal |
| issn | 1754-8403 1754-8411 |
| language | English |
| last_indexed | 2024-12-20T01:19:32Z |
| publishDate | 2019-10-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Disease Models & Mechanisms |
| spelling | doaj.art-09f0f4dcd0da4fc7a999ccb3adeb1ce02022-12-21T19:58:28ZengThe Company of BiologistsDisease Models & Mechanisms1754-84031754-84112019-10-01121010.1242/dmm.039982039982CRISPR/Cas9-mediated genome editing in nonhuman primatesYu Kang0Chu Chu1Fang Wang2Yuyu Niu3 Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650500, China Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China Owing to their high similarity to humans, non-human primates (NHPs) provide an exceedingly suitable model for the study of human disease. In this Review, we summarize the history of transgenic NHP models and the progress of CRISPR/Cas9-mediated genome editing in NHPs, from the first proof-of-principle green fluorescent protein-expressing monkeys to sophisticated NHP models of human neurodegenerative disease that accurately phenocopy several complex disease features. We discuss not only the breakthroughs and advantages, but also the potential shortcomings of the application of the CRISPR/Cas9 system to NHPs that have emerged from the expanded understanding of this technology in recent years. Although off-target and mosaic mutations are the main concerns in CRISPR/Cas9-mediated NHP modeling, recent progress in genome editing techniques make it likely that these technical limitations will be overcome soon, bringing excellent prospects to human disease studies.http://dmm.biologists.org/content/12/10/dmm039982genome editingcrispr/cas9nonhuman primates |
| spellingShingle | Yu Kang Chu Chu Fang Wang Yuyu Niu CRISPR/Cas9-mediated genome editing in nonhuman primates Disease Models & Mechanisms genome editing crispr/cas9 nonhuman primates |
| title | CRISPR/Cas9-mediated genome editing in nonhuman primates |
| title_full | CRISPR/Cas9-mediated genome editing in nonhuman primates |
| title_fullStr | CRISPR/Cas9-mediated genome editing in nonhuman primates |
| title_full_unstemmed | CRISPR/Cas9-mediated genome editing in nonhuman primates |
| title_short | CRISPR/Cas9-mediated genome editing in nonhuman primates |
| title_sort | crispr cas9 mediated genome editing in nonhuman primates |
| topic | genome editing crispr/cas9 nonhuman primates |
| url | http://dmm.biologists.org/content/12/10/dmm039982 |
| work_keys_str_mv | AT yukang crisprcas9mediatedgenomeeditinginnonhumanprimates AT chuchu crisprcas9mediatedgenomeeditinginnonhumanprimates AT fangwang crisprcas9mediatedgenomeeditinginnonhumanprimates AT yuyuniu crisprcas9mediatedgenomeeditinginnonhumanprimates |