Determination of total bacterial count in raw milk by flow cytometry
The automatic flow cytometry as routine method for total bacterial count determination of raw ex-farm milk has recently been accepted in Croatia. This method significantly differs from the reference method (Standard Plate Count) mostly in the presentation of the results obtained. Therefore, this pap...
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Format: | Article |
Language: | English |
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Croatian Dairy Union
2004-01-01
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Series: | Mljekarstvo |
Subjects: | |
Online Access: | http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4022 |
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author | Dubravka Samaržija Neven Antunac Tomislav Pogačić Sanja Sikora |
author_facet | Dubravka Samaržija Neven Antunac Tomislav Pogačić Sanja Sikora |
author_sort | Dubravka Samaržija |
collection | DOAJ |
description | The automatic flow cytometry as routine method for total bacterial count determination of raw ex-farm milk has recently been accepted in Croatia. This method significantly differs from the reference method (Standard Plate Count) mostly in the presentation of the results obtained. Therefore, this paper summarized experiences in the application of flow cytometry in the dairy laboratories practice. The principle and the practice of the method, methodological details and factors influencing the results were described. In order to avoid problems regarding the interpretation of the results, which aregeneral problems of the quantitative microbiology, this article try to explain an appropriate conversion of the results with regards to SPC/ml, as an official method for the bacteriological quality proposal by the national legislation. |
first_indexed | 2024-12-11T18:49:15Z |
format | Article |
id | doaj.art-09f2a82ae22e43619f6fa66621dc3dce |
institution | Directory Open Access Journal |
issn | 0026-704X 1846-4025 |
language | English |
last_indexed | 2024-12-11T18:49:15Z |
publishDate | 2004-01-01 |
publisher | Croatian Dairy Union |
record_format | Article |
series | Mljekarstvo |
spelling | doaj.art-09f2a82ae22e43619f6fa66621dc3dce2022-12-22T00:54:21ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252004-01-015413951Determination of total bacterial count in raw milk by flow cytometryDubravka SamaržijaNeven AntunacTomislav PogačićSanja SikoraThe automatic flow cytometry as routine method for total bacterial count determination of raw ex-farm milk has recently been accepted in Croatia. This method significantly differs from the reference method (Standard Plate Count) mostly in the presentation of the results obtained. Therefore, this paper summarized experiences in the application of flow cytometry in the dairy laboratories practice. The principle and the practice of the method, methodological details and factors influencing the results were described. In order to avoid problems regarding the interpretation of the results, which aregeneral problems of the quantitative microbiology, this article try to explain an appropriate conversion of the results with regards to SPC/ml, as an official method for the bacteriological quality proposal by the national legislation.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4022total bacterial countflow cytometryaccuracy of the method |
spellingShingle | Dubravka Samaržija Neven Antunac Tomislav Pogačić Sanja Sikora Determination of total bacterial count in raw milk by flow cytometry Mljekarstvo total bacterial count flow cytometry accuracy of the method |
title | Determination of total bacterial count in raw milk by flow cytometry |
title_full | Determination of total bacterial count in raw milk by flow cytometry |
title_fullStr | Determination of total bacterial count in raw milk by flow cytometry |
title_full_unstemmed | Determination of total bacterial count in raw milk by flow cytometry |
title_short | Determination of total bacterial count in raw milk by flow cytometry |
title_sort | determination of total bacterial count in raw milk by flow cytometry |
topic | total bacterial count flow cytometry accuracy of the method |
url | http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4022 |
work_keys_str_mv | AT dubravkasamarzija determinationoftotalbacterialcountinrawmilkbyflowcytometry AT nevenantunac determinationoftotalbacterialcountinrawmilkbyflowcytometry AT tomislavpogacic determinationoftotalbacterialcountinrawmilkbyflowcytometry AT sanjasikora determinationoftotalbacterialcountinrawmilkbyflowcytometry |