Determination of total bacterial count in raw milk by flow cytometry

The automatic flow cytometry as routine method for total bacterial count determination of raw ex-farm milk has recently been accepted in Croatia. This method significantly differs from the reference method (Standard Plate Count) mostly in the presentation of the results obtained. Therefore, this pap...

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Main Authors: Dubravka Samaržija, Neven Antunac, Tomislav Pogačić, Sanja Sikora
Format: Article
Language:English
Published: Croatian Dairy Union 2004-01-01
Series:Mljekarstvo
Subjects:
Online Access:http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4022
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author Dubravka Samaržija
Neven Antunac
Tomislav Pogačić
Sanja Sikora
author_facet Dubravka Samaržija
Neven Antunac
Tomislav Pogačić
Sanja Sikora
author_sort Dubravka Samaržija
collection DOAJ
description The automatic flow cytometry as routine method for total bacterial count determination of raw ex-farm milk has recently been accepted in Croatia. This method significantly differs from the reference method (Standard Plate Count) mostly in the presentation of the results obtained. Therefore, this paper summarized experiences in the application of flow cytometry in the dairy laboratories practice. The principle and the practice of the method, methodological details and factors influencing the results were described. In order to avoid problems regarding the interpretation of the results, which aregeneral problems of the quantitative microbiology, this article try to explain an appropriate conversion of the results with regards to SPC/ml, as an official method for the bacteriological quality proposal by the national legislation.
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spelling doaj.art-09f2a82ae22e43619f6fa66621dc3dce2022-12-22T00:54:21ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252004-01-015413951Determination of total bacterial count in raw milk by flow cytometryDubravka SamaržijaNeven AntunacTomislav PogačićSanja SikoraThe automatic flow cytometry as routine method for total bacterial count determination of raw ex-farm milk has recently been accepted in Croatia. This method significantly differs from the reference method (Standard Plate Count) mostly in the presentation of the results obtained. Therefore, this paper summarized experiences in the application of flow cytometry in the dairy laboratories practice. The principle and the practice of the method, methodological details and factors influencing the results were described. In order to avoid problems regarding the interpretation of the results, which aregeneral problems of the quantitative microbiology, this article try to explain an appropriate conversion of the results with regards to SPC/ml, as an official method for the bacteriological quality proposal by the national legislation.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4022total bacterial countflow cytometryaccuracy of the method
spellingShingle Dubravka Samaržija
Neven Antunac
Tomislav Pogačić
Sanja Sikora
Determination of total bacterial count in raw milk by flow cytometry
Mljekarstvo
total bacterial count
flow cytometry
accuracy of the method
title Determination of total bacterial count in raw milk by flow cytometry
title_full Determination of total bacterial count in raw milk by flow cytometry
title_fullStr Determination of total bacterial count in raw milk by flow cytometry
title_full_unstemmed Determination of total bacterial count in raw milk by flow cytometry
title_short Determination of total bacterial count in raw milk by flow cytometry
title_sort determination of total bacterial count in raw milk by flow cytometry
topic total bacterial count
flow cytometry
accuracy of the method
url http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4022
work_keys_str_mv AT dubravkasamarzija determinationoftotalbacterialcountinrawmilkbyflowcytometry
AT nevenantunac determinationoftotalbacterialcountinrawmilkbyflowcytometry
AT tomislavpogacic determinationoftotalbacterialcountinrawmilkbyflowcytometry
AT sanjasikora determinationoftotalbacterialcountinrawmilkbyflowcytometry