Rapid diagnosis of pulmonary tuberculosis
INTRODUCTION: World Health Organization had estimated 9.4 million tuberculosis cases on 2009, with 1.7 million of deaths as consequence of treatment and diagnosis failures. Improving diagnostic methods for the rapid and timely detection of tuberculosis patients is critical to control the disease. Th...
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| Format: | Article |
| Language: | English |
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The Pan African Medical Journal
2014-06-01
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| Series: | The Pan African Medical Journal |
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https://www.panafrican-med-journal.com/content/article/18/141/pdf/141.pdf
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| _version_ | 1818975461072437248 |
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| author | José Mauricio Hernéndez Sarmiento Natalia Builes Restrepo Gloria Isabel Mejéa Elsa Zapata Mary Alejandra Restrepo Jaime Robledo' |
| author_facet | José Mauricio Hernéndez Sarmiento Natalia Builes Restrepo Gloria Isabel Mejéa Elsa Zapata Mary Alejandra Restrepo Jaime Robledo' |
| author_sort | José Mauricio Hernéndez Sarmiento |
| collection | DOAJ |
| description | INTRODUCTION: World Health Organization had estimated 9.4 million tuberculosis cases on 2009, with 1.7 million of deaths as consequence of treatment and diagnosis failures. Improving diagnostic methods for the rapid and timely detection of tuberculosis patients is critical to control the disease. The aim of this study was evaluating the accuracy of the cord factor detection on the solid medium Middlebrook 7H11 thin layer agar compared to the Lowenstein Jensen medium for the rapid tuberculosis diagnosis. METHODS: Patients with suspected tuberculosis were enrolled and their sputum samples were processed for direct smear and culture on Lowenstein Jensen and BACTEC MGIT 960, from which positive tubes were subcultured on Middlebrook 7H11 thin layer agar. Statistical analysis was performed comparing culture results from Lowenstein Jensen and the thin layer agar, and their corresponding average times for detecting Mycobacterium tuberculosis. The performance of cord factor detection was evaluated determining its sensitivity, specificity, positive and negative predictive value. RESULTS: 111 out of 260 patients were positive for M. tuberculosis by Lowenstein Jensen medium with an average time '' standard deviation for its detection of 22.3 , 8.5 days. 115 patients were positive by the MGIT system identifying the cord factor by the Middlebrook 7H11 thin layer agar which average time standard deviation was 5.5 , 2.6 days. CONCLUSION: The cord factor detection by Middlebrook 7H11 thin layer agar allows early and accurate tuberculosis diagnosis during an average time of 5 days, making this rapid diagnosis particularly important in patients with negative sputum smear. |
| first_indexed | 2024-12-20T15:56:19Z |
| format | Article |
| id | doaj.art-09f2a96357e84873a2d898c1e9b7f34e |
| institution | Directory Open Access Journal |
| issn | 1937-8688 1937-8688 |
| language | English |
| last_indexed | 2024-12-20T15:56:19Z |
| publishDate | 2014-06-01 |
| publisher | The Pan African Medical Journal |
| record_format | Article |
| series | The Pan African Medical Journal |
| spelling | doaj.art-09f2a96357e84873a2d898c1e9b7f34e2022-12-21T19:34:28ZengThe Pan African Medical JournalThe Pan African Medical Journal1937-86881937-86882014-06-011814110.11604/pamj.2014.18.141.22952295Rapid diagnosis of pulmonary tuberculosisJosé Mauricio Hernéndez Sarmiento0Natalia Builes Restrepo1Gloria Isabel Mejéa2Elsa Zapata3Mary Alejandra Restrepo4Jaime Robledo'5 School of Health Sciences, Universidad Pontificia Bolivariana (UPB), Medellén, Colombia Bacteriology and Mycobacteriology Unit, Corporacién para Investigaciones Biolégicas (CIB), Medellén, Colombia School of Health Sciences, Universidad Pontificia Bolivariana (UPB), Medellén, Colombia Bacteriology and Mycobacteriology Unit, Corporacién para Investigaciones Biolégicas (CIB), Medellén, Colombia Bacteriology and Mycobacteriology Unit, Corporacién para Investigaciones Biolégicas (CIB), Medellén, Colombia School of Health Sciences, Universidad Pontificia Bolivariana (UPB), Medellén, Colombia INTRODUCTION: World Health Organization had estimated 9.4 million tuberculosis cases on 2009, with 1.7 million of deaths as consequence of treatment and diagnosis failures. Improving diagnostic methods for the rapid and timely detection of tuberculosis patients is critical to control the disease. The aim of this study was evaluating the accuracy of the cord factor detection on the solid medium Middlebrook 7H11 thin layer agar compared to the Lowenstein Jensen medium for the rapid tuberculosis diagnosis. METHODS: Patients with suspected tuberculosis were enrolled and their sputum samples were processed for direct smear and culture on Lowenstein Jensen and BACTEC MGIT 960, from which positive tubes were subcultured on Middlebrook 7H11 thin layer agar. Statistical analysis was performed comparing culture results from Lowenstein Jensen and the thin layer agar, and their corresponding average times for detecting Mycobacterium tuberculosis. The performance of cord factor detection was evaluated determining its sensitivity, specificity, positive and negative predictive value. RESULTS: 111 out of 260 patients were positive for M. tuberculosis by Lowenstein Jensen medium with an average time '' standard deviation for its detection of 22.3 , 8.5 days. 115 patients were positive by the MGIT system identifying the cord factor by the Middlebrook 7H11 thin layer agar which average time standard deviation was 5.5 , 2.6 days. CONCLUSION: The cord factor detection by Middlebrook 7H11 thin layer agar allows early and accurate tuberculosis diagnosis during an average time of 5 days, making this rapid diagnosis particularly important in patients with negative sputum smear. https://www.panafrican-med-journal.com/content/article/18/141/pdf/141.pdf cord factortuberculosismgitthin layer agar |
| spellingShingle | José Mauricio Hernéndez Sarmiento Natalia Builes Restrepo Gloria Isabel Mejéa Elsa Zapata Mary Alejandra Restrepo Jaime Robledo' Rapid diagnosis of pulmonary tuberculosis The Pan African Medical Journal cord factor tuberculosis mgit thin layer agar |
| title | Rapid diagnosis of pulmonary tuberculosis |
| title_full | Rapid diagnosis of pulmonary tuberculosis |
| title_fullStr | Rapid diagnosis of pulmonary tuberculosis |
| title_full_unstemmed | Rapid diagnosis of pulmonary tuberculosis |
| title_short | Rapid diagnosis of pulmonary tuberculosis |
| title_sort | rapid diagnosis of pulmonary tuberculosis |
| topic | cord factor tuberculosis mgit thin layer agar |
| url |
https://www.panafrican-med-journal.com/content/article/18/141/pdf/141.pdf
|
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