Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis
Abstract Background Serving as a stop signal of inflammation, the role of lipoxin A4 (LXA4) in periodontitis remains to be clarified. This study is aimed to examine the changes in LXA4 levels in gingival crevicular fluid (GCF) after scaling and root planing (SRP) and to determine the relationship be...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-024-03948-w |
| _version_ | 1827325937226612736 |
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| author | Rui Ma Yiying Liu Yi Xu Dingyu Duan |
| author_facet | Rui Ma Yiying Liu Yi Xu Dingyu Duan |
| author_sort | Rui Ma |
| collection | DOAJ |
| description | Abstract Background Serving as a stop signal of inflammation, the role of lipoxin A4 (LXA4) in periodontitis remains to be clarified. This study is aimed to examine the changes in LXA4 levels in gingival crevicular fluid (GCF) after scaling and root planing (SRP) and to determine the relationship between LXA4 levels and treatment outcomes and periodontal pathogens in severe periodontitis. Methods A total of 74 GCF samples were collected from 21 severe periodontitis participants at the deepest affected sites. These sites were re-sampled at 1, 3, and 6 months after SRP. Besides, GCF samples were also collected from 25 periodontally healthy participants. Clinical parameters including probing depth (PD) and clinical attachment level (CAL) in periodontitis group were recorded. LXA4 levels and periodontal pathogens in the GCF were analyzed by ELISA and PCR, respectively. Correlations between GCF LXA4 levels and treatment effect and periodontal pathogens were assessed. Results LXA4 levels in GCF significantly increased after SRP (p < 0.05), but remained lower than those observed in healthy individuals (p < 0.05). Sites with lower baseline LXA4 concentrations were more likely to experience greater improvements in PD at 6 months post-SRP (area under the curve [AUC] = 0.792), and the improvements were positively correlated with the increase of LXA4 at these sites post-treatment (p < 0.05). Furthermore, more elevated LXA4 levels were observed in sites that became negative for Prevotella intermedia or Tannerella forsythia after SRP. Conclusion Baseline LXA4 in GCF has the potential to predict the site-specific response of severe periodontal lesions to SRP. The increase of LXA4 levels after treatment was positively correlated with clinical improvements and negatively correlated with the presence of Prevotella intermedia or Tannerella forsythia. |
| first_indexed | 2024-03-07T14:37:28Z |
| format | Article |
| id | doaj.art-09f2e048a66e41d59ea5409def723577 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-03-07T14:37:28Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-09f2e048a66e41d59ea5409def7235772024-03-05T20:33:07ZengBMCBMC Oral Health1472-68312024-02-0124111010.1186/s12903-024-03948-wLipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitisRui Ma0Yiying Liu1Yi Xu2Dingyu Duan3State Key Laboratory of Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan UniversityState Key Laboratory of Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan UniversityState Key Laboratory of Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan UniversityState Key Laboratory of Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan UniversityAbstract Background Serving as a stop signal of inflammation, the role of lipoxin A4 (LXA4) in periodontitis remains to be clarified. This study is aimed to examine the changes in LXA4 levels in gingival crevicular fluid (GCF) after scaling and root planing (SRP) and to determine the relationship between LXA4 levels and treatment outcomes and periodontal pathogens in severe periodontitis. Methods A total of 74 GCF samples were collected from 21 severe periodontitis participants at the deepest affected sites. These sites were re-sampled at 1, 3, and 6 months after SRP. Besides, GCF samples were also collected from 25 periodontally healthy participants. Clinical parameters including probing depth (PD) and clinical attachment level (CAL) in periodontitis group were recorded. LXA4 levels and periodontal pathogens in the GCF were analyzed by ELISA and PCR, respectively. Correlations between GCF LXA4 levels and treatment effect and periodontal pathogens were assessed. Results LXA4 levels in GCF significantly increased after SRP (p < 0.05), but remained lower than those observed in healthy individuals (p < 0.05). Sites with lower baseline LXA4 concentrations were more likely to experience greater improvements in PD at 6 months post-SRP (area under the curve [AUC] = 0.792), and the improvements were positively correlated with the increase of LXA4 at these sites post-treatment (p < 0.05). Furthermore, more elevated LXA4 levels were observed in sites that became negative for Prevotella intermedia or Tannerella forsythia after SRP. Conclusion Baseline LXA4 in GCF has the potential to predict the site-specific response of severe periodontal lesions to SRP. The increase of LXA4 levels after treatment was positively correlated with clinical improvements and negatively correlated with the presence of Prevotella intermedia or Tannerella forsythia.https://doi.org/10.1186/s12903-024-03948-wLipoxinsPeriodontitisPrognosisRoot planingGingival crevicular fluid |
| spellingShingle | Rui Ma Yiying Liu Yi Xu Dingyu Duan Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis BMC Oral Health Lipoxins Periodontitis Prognosis Root planing Gingival crevicular fluid |
| title | Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis |
| title_full | Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis |
| title_fullStr | Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis |
| title_full_unstemmed | Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis |
| title_short | Lipoxin A4 levels predict site-specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis |
| title_sort | lipoxin a4 levels predict site specific clinical improvements post scaling and root planing and correlate negatively with periodontal pathogens in severe periodontitis |
| topic | Lipoxins Periodontitis Prognosis Root planing Gingival crevicular fluid |
| url | https://doi.org/10.1186/s12903-024-03948-w |
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