Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations
Candida glabrata is an opportunistic fungal pathogen that currently ranks as the second most common cause of candidiasis. Although the mechanisms underlying virulence and drug resistance in C. glabrata are now starting to be elucidated, we still lack a good understanding of how this yeast adapts dur...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00112/full |
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| author | Laia Carreté Laia Carreté Ewa Ksiezopolska Ewa Ksiezopolska Emilia Gómez-Molero Adela Angoulvant Adela Angoulvant Oliver Bader Cécile Fairhead Toni Gabaldón Toni Gabaldón Toni Gabaldón |
| author_facet | Laia Carreté Laia Carreté Ewa Ksiezopolska Ewa Ksiezopolska Emilia Gómez-Molero Adela Angoulvant Adela Angoulvant Oliver Bader Cécile Fairhead Toni Gabaldón Toni Gabaldón Toni Gabaldón |
| author_sort | Laia Carreté |
| collection | DOAJ |
| description | Candida glabrata is an opportunistic fungal pathogen that currently ranks as the second most common cause of candidiasis. Although the mechanisms underlying virulence and drug resistance in C. glabrata are now starting to be elucidated, we still lack a good understanding of how this yeast adapts during the course of an infection. Outstanding questions are whether the observed genomic plasticity of C. glabrata plays a role during infection, or what levels of genetic variation exist within an infecting clonal population. To shed light onto the genomic variation within infecting C. glabrata populations, we compared the genomes of 11 pairs and one trio of serial clinical isolates, each obtained from a single patient. Our results provide a catalog of genetic variations existing within clonal infecting isolates, and reveal an enrichment of non-synonymous changes in genes encoding cell-wall proteins. Genetic variation and the presence of non-synonymous mutations and copy number variations accumulated within the host, suggest that clonal populations entail a non-negligible level of genetic variation that may reflect selection processes that occur within the human body. As we show here, these genomic changes can underlie phenotypic differences in traits that are relevant for infection. |
| first_indexed | 2024-12-23T14:36:57Z |
| format | Article |
| id | doaj.art-09f5092271714ea8b315ed48b739d658 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T14:36:57Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-09f5092271714ea8b315ed48b739d6582022-12-21T17:43:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00112429839Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal PopulationsLaia Carreté0Laia Carreté1Ewa Ksiezopolska2Ewa Ksiezopolska3Emilia Gómez-Molero4Adela Angoulvant5Adela Angoulvant6Oliver Bader7Cécile Fairhead8Toni Gabaldón9Toni Gabaldón10Toni Gabaldón11Bioinformatics and Genomics Programme, Centre for Genomic Regulation, Barcelona Institute of Science and Technology, Barcelona, SpainDepartament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), Barcelona, SpainBioinformatics and Genomics Programme, Centre for Genomic Regulation, Barcelona Institute of Science and Technology, Barcelona, SpainDepartament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), Barcelona, SpainInstitute for Medical Microbiology, University Medical Center Göttingen, Göttingen, GermanyGénétique Quantitative et Évolution Le Moulon, INRA, Université Paris-Sud, CNRS, AgroParisTech, Orsay, FranceParasitology and Mycology Department, Bicêtre University Hospital, Paris-Sud University, Le Kremlin-Bicêtre, FranceInstitute for Medical Microbiology, University Medical Center Göttingen, Göttingen, GermanyGénétique Quantitative et Évolution Le Moulon, INRA, Université Paris-Sud, CNRS, AgroParisTech, Orsay, FranceBioinformatics and Genomics Programme, Centre for Genomic Regulation, Barcelona Institute of Science and Technology, Barcelona, SpainDepartament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), Barcelona, SpainInstitució Catalana de Recerca i Estudis Avançats, Barcelona, SpainCandida glabrata is an opportunistic fungal pathogen that currently ranks as the second most common cause of candidiasis. Although the mechanisms underlying virulence and drug resistance in C. glabrata are now starting to be elucidated, we still lack a good understanding of how this yeast adapts during the course of an infection. Outstanding questions are whether the observed genomic plasticity of C. glabrata plays a role during infection, or what levels of genetic variation exist within an infecting clonal population. To shed light onto the genomic variation within infecting C. glabrata populations, we compared the genomes of 11 pairs and one trio of serial clinical isolates, each obtained from a single patient. Our results provide a catalog of genetic variations existing within clonal infecting isolates, and reveal an enrichment of non-synonymous changes in genes encoding cell-wall proteins. Genetic variation and the presence of non-synonymous mutations and copy number variations accumulated within the host, suggest that clonal populations entail a non-negligible level of genetic variation that may reflect selection processes that occur within the human body. As we show here, these genomic changes can underlie phenotypic differences in traits that are relevant for infection.https://www.frontiersin.org/article/10.3389/fmicb.2019.00112/fullcandidiasisCandida glabrataclinical isolatesresistancegenome sequencinggenome variation |
| spellingShingle | Laia Carreté Laia Carreté Ewa Ksiezopolska Ewa Ksiezopolska Emilia Gómez-Molero Adela Angoulvant Adela Angoulvant Oliver Bader Cécile Fairhead Toni Gabaldón Toni Gabaldón Toni Gabaldón Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations Frontiers in Microbiology candidiasis Candida glabrata clinical isolates resistance genome sequencing genome variation |
| title | Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations |
| title_full | Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations |
| title_fullStr | Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations |
| title_full_unstemmed | Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations |
| title_short | Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations |
| title_sort | genome comparisons of candida glabrata serial clinical isolates reveal patterns of genetic variation in infecting clonal populations |
| topic | candidiasis Candida glabrata clinical isolates resistance genome sequencing genome variation |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00112/full |
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