Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related Interleukins
Hyperglycemia associated with prediabetes (PD) alters NLRP3 inflammasome activity and related interleukins, yet no study has evaluated the expression of the NLRP3 inflammasome complex and related interleukins in individuals with a PD condition that did or did not develop type 2 diabetes mellitus (T2...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13837 |
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| author | Hend Alfadul Shaun Sabico Abdullah M. Alnaami Osama E. Amer Syed D. Hussain Kaiser Wani Mario Clerici Nasser M. Al-Daghri |
| author_facet | Hend Alfadul Shaun Sabico Abdullah M. Alnaami Osama E. Amer Syed D. Hussain Kaiser Wani Mario Clerici Nasser M. Al-Daghri |
| author_sort | Hend Alfadul |
| collection | DOAJ |
| description | Hyperglycemia associated with prediabetes (PD) alters NLRP3 inflammasome activity and related interleukins, yet no study has evaluated the expression of the NLRP3 inflammasome complex and related interleukins in individuals with a PD condition that did or did not develop type 2 diabetes mellitus (T2DM). This study investigated the effect of 6 months of lifestyle modification on the expression of the NLRP3 inflammasome and related interleukins (1α, 1β, 18, 33 and 37) in the sera of individuals with a PD condition that did or did not develop T2DM. This interventional study included 67 Saudi adults (mean age = 41.9 ± 8.0 years, mean BMI = 33.2 ± 5.5 kg/m<sup>2</sup>). Overnight-fasting serum samples were collected at baseline and at the 6-month follow-up. Serum levels of NLRP3, capsase-1 and related ILs were analyzed at both visits using commercially available immunoassay kits. Results showed that IL-1α increased in the PD group that developed T2DM (<i>p</i> = 0.046), IL-33 decreased in the PD group that reverted to normal (<i>p</i> < 0.001) and NLRP3 decreased in the PD group that remained PD (<i>p</i> = 0.01). Results also showed a positive over-time correlation between NLRP3 and both IL-1α and IL-33 (<i>p</i> < 0.001 and <i>p</i> = 0.028, respectively). In conclusion, glycemic control favorably altered NLRP3 inflammasome complex activity, and lifestyle modification in PD individuals is crucial in reversing harmful metabolic and inflammatory phenotypes. |
| first_indexed | 2024-03-10T22:40:53Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:53Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-09f7237fb25d47ee89c304fa865725bd2023-11-19T11:03:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181383710.3390/ijms241813837Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related InterleukinsHend Alfadul0Shaun Sabico1Abdullah M. Alnaami2Osama E. Amer3Syed D. Hussain4Kaiser Wani5Mario Clerici6Nasser M. Al-Daghri7Chair for Biomarkers of Chronic Diseases, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for Biomarkers of Chronic Diseases, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for Biomarkers of Chronic Diseases, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for Biomarkers of Chronic Diseases, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for Biomarkers of Chronic Diseases, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaChair for Biomarkers of Chronic Diseases, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Medical-Surgery Physiopathology and Transplantation, University of Milan, 20122 Milan, ItalyChair for Biomarkers of Chronic Diseases, Biochemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaHyperglycemia associated with prediabetes (PD) alters NLRP3 inflammasome activity and related interleukins, yet no study has evaluated the expression of the NLRP3 inflammasome complex and related interleukins in individuals with a PD condition that did or did not develop type 2 diabetes mellitus (T2DM). This study investigated the effect of 6 months of lifestyle modification on the expression of the NLRP3 inflammasome and related interleukins (1α, 1β, 18, 33 and 37) in the sera of individuals with a PD condition that did or did not develop T2DM. This interventional study included 67 Saudi adults (mean age = 41.9 ± 8.0 years, mean BMI = 33.2 ± 5.5 kg/m<sup>2</sup>). Overnight-fasting serum samples were collected at baseline and at the 6-month follow-up. Serum levels of NLRP3, capsase-1 and related ILs were analyzed at both visits using commercially available immunoassay kits. Results showed that IL-1α increased in the PD group that developed T2DM (<i>p</i> = 0.046), IL-33 decreased in the PD group that reverted to normal (<i>p</i> < 0.001) and NLRP3 decreased in the PD group that remained PD (<i>p</i> = 0.01). Results also showed a positive over-time correlation between NLRP3 and both IL-1α and IL-33 (<i>p</i> < 0.001 and <i>p</i> = 0.028, respectively). In conclusion, glycemic control favorably altered NLRP3 inflammasome complex activity, and lifestyle modification in PD individuals is crucial in reversing harmful metabolic and inflammatory phenotypes.https://www.mdpi.com/1422-0067/24/18/13837prediabetestype 2 diabetes mellitusNLRP3inflammasomesinterleukinschronic inflammation |
| spellingShingle | Hend Alfadul Shaun Sabico Abdullah M. Alnaami Osama E. Amer Syed D. Hussain Kaiser Wani Mario Clerici Nasser M. Al-Daghri Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related Interleukins International Journal of Molecular Sciences prediabetes type 2 diabetes mellitus NLRP3 inflammasomes interleukins chronic inflammation |
| title | Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related Interleukins |
| title_full | Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related Interleukins |
| title_fullStr | Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related Interleukins |
| title_full_unstemmed | Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related Interleukins |
| title_short | Acute Glycemic Control in Prediabetes Individuals Favorably Alters Serum NLRP3 Inflammasome and Related Interleukins |
| title_sort | acute glycemic control in prediabetes individuals favorably alters serum nlrp3 inflammasome and related interleukins |
| topic | prediabetes type 2 diabetes mellitus NLRP3 inflammasomes interleukins chronic inflammation |
| url | https://www.mdpi.com/1422-0067/24/18/13837 |
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