Expression of KLK2 gene in prostate cancer
Background: Prostate cancer is one of the most common diseases that affect men. Although prostate cancer is not the fatal flaw in most cases, detection of effective factors for early diagnosis and treatment is essential. Research results have shown that the use of KLK2 plus PSA can be a good biomark...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2018-01-01
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| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5649&slc_lang=en&sid=1 |
| _version_ | 1818900587396202496 |
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| author | Sajad Shafai Elham Moslemi Mehdi Mohammadi Kasra Esfahani Amir Izadi |
| author_facet | Sajad Shafai Elham Moslemi Mehdi Mohammadi Kasra Esfahani Amir Izadi |
| author_sort | Sajad Shafai |
| collection | DOAJ |
| description | Background: Prostate cancer is one of the most common diseases that affect men. Although prostate cancer is not the fatal flaw in most cases, detection of effective factors for early diagnosis and treatment is essential. Research results have shown that the use of KLK2 plus PSA can be a good biomarker for diagnosing prostate cancer. During prostate cancer, expression of KLK2 gene increases which can be used as a prostate cancer biomarker. The aim of this study is an assessment of KLK2 gene expression as a potential factor in the prostate cancer diagnosis.
Methods: In this case study, 50 prostate cancer urine samples from patients and 50 urine samples from normal individuals who were referred to Mehr Hospital of Tehran (from December 2014 to February 2016) were obtained and stored in the central research laboratory of Shahid Beheshti University of Medical Sciences, Tehran, till tests were being done. The age of collected samples between the 46 up to 71 years. RNA of samples were extracted, and then cDNA was synthesized by using M-MuLV enzyme, Oligo dt, and Random hexamer primers. KLK2 specific primers designed by Primer Express software, version 3.0 (Applied Biosystems, Foster City, CA, USA), and KLK2 gene expression evaluated by using ∆∆ct methods.
Results: In comparison with patients and normal sample`s gene expression, the mean increase expression of KLK2 gene in patients less than 50 years was 2.32 and in patients more than 50 years, it was 5.79, P<0.0001. In addition, gene expression results with respect to GS (Gleason grading system) classification shown that patients with GS6 had the lowest gene expression (3.40) and in the patients with GS8, had the highest gene expression (10.74) in comparison with normal group (P<0.0001).
Conclusion: The expression of KLK2 gene in people with prostate cancer is the higher than the healthy person; finally, according to the results, it could be mentioned that the KLK2 gene considered as a useful factor in prostate cancer, whose expression is associated with progression and development of the prostate cancer. |
| first_indexed | 2024-12-19T20:06:14Z |
| format | Article |
| id | doaj.art-09f839b8d1a0424ca2b643962d3b0a96 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-19T20:06:14Z |
| publishDate | 2018-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-09f839b8d1a0424ca2b643962d3b0a962022-12-21T20:07:29ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222018-01-017510745751Expression of KLK2 gene in prostate cancerSajad Shafai0Elham Moslemi1Mehdi Mohammadi2Kasra Esfahani3Amir Izadi4 Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran. Department of Biology, Islamic Azad University, East Tehran Branch, Tehran, Iran. Department of Bioprocess Engineering, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. Department of Plant Bioproducts, Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. Young Researcher and Elite Club, Islamic Azad University, East Tehran Branch, Tehran, Iran. Background: Prostate cancer is one of the most common diseases that affect men. Although prostate cancer is not the fatal flaw in most cases, detection of effective factors for early diagnosis and treatment is essential. Research results have shown that the use of KLK2 plus PSA can be a good biomarker for diagnosing prostate cancer. During prostate cancer, expression of KLK2 gene increases which can be used as a prostate cancer biomarker. The aim of this study is an assessment of KLK2 gene expression as a potential factor in the prostate cancer diagnosis. Methods: In this case study, 50 prostate cancer urine samples from patients and 50 urine samples from normal individuals who were referred to Mehr Hospital of Tehran (from December 2014 to February 2016) were obtained and stored in the central research laboratory of Shahid Beheshti University of Medical Sciences, Tehran, till tests were being done. The age of collected samples between the 46 up to 71 years. RNA of samples were extracted, and then cDNA was synthesized by using M-MuLV enzyme, Oligo dt, and Random hexamer primers. KLK2 specific primers designed by Primer Express software, version 3.0 (Applied Biosystems, Foster City, CA, USA), and KLK2 gene expression evaluated by using ∆∆ct methods. Results: In comparison with patients and normal sample`s gene expression, the mean increase expression of KLK2 gene in patients less than 50 years was 2.32 and in patients more than 50 years, it was 5.79, P<0.0001. In addition, gene expression results with respect to GS (Gleason grading system) classification shown that patients with GS6 had the lowest gene expression (3.40) and in the patients with GS8, had the highest gene expression (10.74) in comparison with normal group (P<0.0001). Conclusion: The expression of KLK2 gene in people with prostate cancer is the higher than the healthy person; finally, according to the results, it could be mentioned that the KLK2 gene considered as a useful factor in prostate cancer, whose expression is associated with progression and development of the prostate cancer.http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5649&slc_lang=en&sid=1gene expression KLK2 prostate cancer real-time polymerase chain reaction |
| spellingShingle | Sajad Shafai Elham Moslemi Mehdi Mohammadi Kasra Esfahani Amir Izadi Expression of KLK2 gene in prostate cancer Tehran University Medical Journal gene expression KLK2 prostate cancer real-time polymerase chain reaction |
| title | Expression of KLK2 gene in prostate cancer |
| title_full | Expression of KLK2 gene in prostate cancer |
| title_fullStr | Expression of KLK2 gene in prostate cancer |
| title_full_unstemmed | Expression of KLK2 gene in prostate cancer |
| title_short | Expression of KLK2 gene in prostate cancer |
| title_sort | expression of klk2 gene in prostate cancer |
| topic | gene expression KLK2 prostate cancer real-time polymerase chain reaction |
| url | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5649&slc_lang=en&sid=1 |
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