Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying <i>S. epidermidis</i>
<i>Staphylococcus epidermidis</i> (<i>S. epidermidis</i>) belongs to methicillin-resistant bacteria strains that cause severe disease in humans. Herein, molecularly imprinted polymer (MIP) nanoparticles resulting from solid-phase synthesis on entire cells were employed as a s...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/23/7/3526 |
| _version_ | 1797607020842450944 |
|---|---|
| author | Chularat Hlaoperm Wisnu Arfian A. Sudjarwo Jakob Ehrenbrandtner Endre Kiss Giorgia Del Favero Kiattawee Choowongkomon Jatuporn Rattanasrisomporn Peter A. Lieberzeit |
| author_facet | Chularat Hlaoperm Wisnu Arfian A. Sudjarwo Jakob Ehrenbrandtner Endre Kiss Giorgia Del Favero Kiattawee Choowongkomon Jatuporn Rattanasrisomporn Peter A. Lieberzeit |
| author_sort | Chularat Hlaoperm |
| collection | DOAJ |
| description | <i>Staphylococcus epidermidis</i> (<i>S. epidermidis</i>) belongs to methicillin-resistant bacteria strains that cause severe disease in humans. Herein, molecularly imprinted polymer (MIP) nanoparticles resulting from solid-phase synthesis on entire cells were employed as a sensing material to identify the species. MIP nanoparticles revealed spherical shapes with diameters of approximately 70 nm to 200 nm in scanning electron microscopy (SEM), which atomic force microscopy (AFM) confirmed. The interaction between nanoparticles and bacteria was assessed using height image analysis in AFM. Selective binding between MIP nanoparticles and <i>S. epidermidis</i> leads to uneven surfaces on bacteria. The surface roughness of <i>S. epidermidis</i> cells was increased to approximately 6.3 ± 1.2 nm after binding to MIP nanoparticles from around 1 nm in the case of native cells. This binding behavior is selective: when exposing <i>Escherichia coli</i> and <i>Bacillus subtilis</i> to the same MIP nanoparticle solutions, one cannot observe binding. Fluorescence microscopy confirms both sensitivity and selectivity. Hence, the developed MIP nanoparticles are a promising approach to identify (pathogenic) bacteria species. |
| first_indexed | 2024-03-11T05:24:26Z |
| format | Article |
| id | doaj.art-09f84e8da25f46e28fe7ec4dbeb8f385 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T05:24:26Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-09f84e8da25f46e28fe7ec4dbeb8f3852023-11-17T17:33:50ZengMDPI AGSensors1424-82202023-03-01237352610.3390/s23073526Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying <i>S. epidermidis</i>Chularat Hlaoperm0Wisnu Arfian A. Sudjarwo1Jakob Ehrenbrandtner2Endre Kiss3Giorgia Del Favero4Kiattawee Choowongkomon5Jatuporn Rattanasrisomporn6Peter A. Lieberzeit7University of Vienna, Faculty for Chemistry, Department of Physical Chemistry, Waehringer Strasse 42, A-1090 Wien, AustriaUniversity of Vienna, Faculty for Chemistry, Department of Physical Chemistry, Waehringer Strasse 42, A-1090 Wien, AustriaUniversity of Vienna, Faculty for Chemistry, Department of Physical Chemistry, Waehringer Strasse 42, A-1090 Wien, AustriaUniversity of Vienna, Faculty for Chemistry, Core Facility Multimodal Imaging, Waehringer Strasse 38, A-1090 Vienna, AustriaUniversity of Vienna, Faculty for Chemistry, Core Facility Multimodal Imaging, Waehringer Strasse 38, A-1090 Vienna, AustriaDepartment of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandCenter for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, ThailandUniversity of Vienna, Faculty for Chemistry, Department of Physical Chemistry, Waehringer Strasse 42, A-1090 Wien, Austria<i>Staphylococcus epidermidis</i> (<i>S. epidermidis</i>) belongs to methicillin-resistant bacteria strains that cause severe disease in humans. Herein, molecularly imprinted polymer (MIP) nanoparticles resulting from solid-phase synthesis on entire cells were employed as a sensing material to identify the species. MIP nanoparticles revealed spherical shapes with diameters of approximately 70 nm to 200 nm in scanning electron microscopy (SEM), which atomic force microscopy (AFM) confirmed. The interaction between nanoparticles and bacteria was assessed using height image analysis in AFM. Selective binding between MIP nanoparticles and <i>S. epidermidis</i> leads to uneven surfaces on bacteria. The surface roughness of <i>S. epidermidis</i> cells was increased to approximately 6.3 ± 1.2 nm after binding to MIP nanoparticles from around 1 nm in the case of native cells. This binding behavior is selective: when exposing <i>Escherichia coli</i> and <i>Bacillus subtilis</i> to the same MIP nanoparticle solutions, one cannot observe binding. Fluorescence microscopy confirms both sensitivity and selectivity. Hence, the developed MIP nanoparticles are a promising approach to identify (pathogenic) bacteria species.https://www.mdpi.com/1424-8220/23/7/3526AFMmolecularly imprinted polymersnanoparticles<i>Staphylococcus epidermidis</i> |
| spellingShingle | Chularat Hlaoperm Wisnu Arfian A. Sudjarwo Jakob Ehrenbrandtner Endre Kiss Giorgia Del Favero Kiattawee Choowongkomon Jatuporn Rattanasrisomporn Peter A. Lieberzeit Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying <i>S. epidermidis</i> Sensors AFM molecularly imprinted polymers nanoparticles <i>Staphylococcus epidermidis</i> |
| title | Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying <i>S. epidermidis</i> |
| title_full | Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying <i>S. epidermidis</i> |
| title_fullStr | Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying <i>S. epidermidis</i> |
| title_full_unstemmed | Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying <i>S. epidermidis</i> |
| title_short | Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying <i>S. epidermidis</i> |
| title_sort | molecularly imprinted nanoparticle ensembles for rapidly identifying i s epidermidis i |
| topic | AFM molecularly imprinted polymers nanoparticles <i>Staphylococcus epidermidis</i> |
| url | https://www.mdpi.com/1424-8220/23/7/3526 |
| work_keys_str_mv | AT chularathlaoperm molecularlyimprintednanoparticleensemblesforrapidlyidentifyingisepidermidisi AT wisnuarfianasudjarwo molecularlyimprintednanoparticleensemblesforrapidlyidentifyingisepidermidisi AT jakobehrenbrandtner molecularlyimprintednanoparticleensemblesforrapidlyidentifyingisepidermidisi AT endrekiss molecularlyimprintednanoparticleensemblesforrapidlyidentifyingisepidermidisi AT giorgiadelfavero molecularlyimprintednanoparticleensemblesforrapidlyidentifyingisepidermidisi AT kiattaweechoowongkomon molecularlyimprintednanoparticleensemblesforrapidlyidentifyingisepidermidisi AT jatupornrattanasrisomporn molecularlyimprintednanoparticleensemblesforrapidlyidentifyingisepidermidisi AT peteralieberzeit molecularlyimprintednanoparticleensemblesforrapidlyidentifyingisepidermidisi |