Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis.
The ability to integrate environmental and developmental signals with physiological responses is critical for plant survival. How this integration is done, particularly through posttranscriptional control of gene expression, is poorly understood. Previously, it was found that the 30 kD subunit of Ar...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4278772?pdf=render |
| _version_ | 1830382614253404160 |
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| author | Man Liu Ruqiang Xu Carrie Merrill Liwei Hong Carol Von Lanken Arthur G Hunt Qingshun Q Li |
| author_facet | Man Liu Ruqiang Xu Carrie Merrill Liwei Hong Carol Von Lanken Arthur G Hunt Qingshun Q Li |
| author_sort | Man Liu |
| collection | DOAJ |
| description | The ability to integrate environmental and developmental signals with physiological responses is critical for plant survival. How this integration is done, particularly through posttranscriptional control of gene expression, is poorly understood. Previously, it was found that the 30 kD subunit of Arabidopsis cleavage and polyadenylation specificity factor (AtCPSF30) is a calmodulin-regulated RNA-binding protein. Here we demonstrated that mutant plants (oxt6) deficient in AtCPSF30 possess a novel range of phenotypes--reduced fertility, reduced lateral root formation, and altered sensitivities to oxidative stress and a number of plant hormones (auxin, cytokinin, gibberellic acid, and ACC). While the wild-type AtCPSF30 (C30G) was able to restore normal growth and responses, a mutant AtCPSF30 protein incapable of interacting with calmodulin (C30GM) could only restore wild-type fertility and responses to oxidative stress and ACC. Thus, the interaction with calmodulin is important for part of AtCPSF30 functions in the plant. Global poly(A) site analysis showed that the C30G and C30GM proteins can restore wild-type poly(A) site choice to the oxt6 mutant. Genes associated with hormone metabolism and auxin responses are also affected by the oxt6 mutation. Moreover, 19 genes that are linked with calmodulin-dependent CPSF30 functions, were identified through genome-wide expression analysis. These data, in conjunction with previous results from the analysis of the oxt6 mutant, indicate that the polyadenylation factor AtCPSF30 is a regulatory hub where different signaling cues are transduced, presumably via differential mRNA 3' end formation or alternative polyadenylation, into specified phenotypic outcomes. Our results suggest a novel function of a polyadenylation factor in environmental and developmental signal integration. |
| first_indexed | 2024-12-20T10:09:46Z |
| format | Article |
| id | doaj.art-09f8ea2a97b347be94c6b968f0618ef8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T10:09:46Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-09f8ea2a97b347be94c6b968f0618ef82022-12-21T19:44:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11577910.1371/journal.pone.0115779Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis.Man LiuRuqiang XuCarrie MerrillLiwei HongCarol Von LankenArthur G HuntQingshun Q LiThe ability to integrate environmental and developmental signals with physiological responses is critical for plant survival. How this integration is done, particularly through posttranscriptional control of gene expression, is poorly understood. Previously, it was found that the 30 kD subunit of Arabidopsis cleavage and polyadenylation specificity factor (AtCPSF30) is a calmodulin-regulated RNA-binding protein. Here we demonstrated that mutant plants (oxt6) deficient in AtCPSF30 possess a novel range of phenotypes--reduced fertility, reduced lateral root formation, and altered sensitivities to oxidative stress and a number of plant hormones (auxin, cytokinin, gibberellic acid, and ACC). While the wild-type AtCPSF30 (C30G) was able to restore normal growth and responses, a mutant AtCPSF30 protein incapable of interacting with calmodulin (C30GM) could only restore wild-type fertility and responses to oxidative stress and ACC. Thus, the interaction with calmodulin is important for part of AtCPSF30 functions in the plant. Global poly(A) site analysis showed that the C30G and C30GM proteins can restore wild-type poly(A) site choice to the oxt6 mutant. Genes associated with hormone metabolism and auxin responses are also affected by the oxt6 mutation. Moreover, 19 genes that are linked with calmodulin-dependent CPSF30 functions, were identified through genome-wide expression analysis. These data, in conjunction with previous results from the analysis of the oxt6 mutant, indicate that the polyadenylation factor AtCPSF30 is a regulatory hub where different signaling cues are transduced, presumably via differential mRNA 3' end formation or alternative polyadenylation, into specified phenotypic outcomes. Our results suggest a novel function of a polyadenylation factor in environmental and developmental signal integration.http://europepmc.org/articles/PMC4278772?pdf=render |
| spellingShingle | Man Liu Ruqiang Xu Carrie Merrill Liwei Hong Carol Von Lanken Arthur G Hunt Qingshun Q Li Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis. PLoS ONE |
| title | Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis. |
| title_full | Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis. |
| title_fullStr | Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis. |
| title_full_unstemmed | Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis. |
| title_short | Integration of developmental and environmental signals via a polyadenylation factor in Arabidopsis. |
| title_sort | integration of developmental and environmental signals via a polyadenylation factor in arabidopsis |
| url | http://europepmc.org/articles/PMC4278772?pdf=render |
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