Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.)
AbstractJerusalem artichoke (Helianthus tuberosus L.) is a fructan-accumulating plant and an industrial raw material for fructan production. However, the genetic mechanism of fructan biosynthesis remains unclear. Therefore, this study performed transcriptome analysis to determine the genetic differe...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2022.2098056 |
| _version_ | 1797974103724916736 |
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| author | Ying Wang Gui Wang Zhenjie Zhou Yuan Zong Lihui Wang Shipeng Yang Yaqi Zhang Xuemei Sun |
| author_facet | Ying Wang Gui Wang Zhenjie Zhou Yuan Zong Lihui Wang Shipeng Yang Yaqi Zhang Xuemei Sun |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | AbstractJerusalem artichoke (Helianthus tuberosus L.) is a fructan-accumulating plant and an industrial raw material for fructan production. However, the genetic mechanism of fructan biosynthesis remains unclear. Therefore, this study performed transcriptome analysis to determine the genetic differences between two Jerusalem artichoke genotypes and different fructan contents. Approximately 19.73 Gb of clean data were obtained after filtering, and a total of 164,006 unigenes were annotated based on GO, KOG and KEGG functional classification. Then, seven homologous genes involved in fructan biosynthesis were obtained by homology comparison. CL11458.Contig2 and CL7122.Contig14 were candidate genes for sucrose:sucrose 1-fructosyl-transferase (1-SST) and fructan:fructan 1-fructosyl-transferase (1-FFT), respectively. The genetic relationship between SST and SST from artichoke in the phylogenetic tree are relatively close, and FFT is located in the same branch as FFT from ‘Qingyu No.1’ Jerusalem artichoke and Viguiera. SST and FFT were the main regulatory genes of fructan accumulation in Jerusalem artichoke. Therefore, this study successfully identified major genes involved in fructan biosynthesis. Future studies should aim to prove the role of each candidate gene in fructan biosynthesis in Jerusalem artichoke. |
| first_indexed | 2024-04-11T04:14:45Z |
| format | Article |
| id | doaj.art-09fb1578b8ea424e850577532878a562 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-04-11T04:14:45Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-09fb1578b8ea424e850577532878a5622022-12-31T18:03:17ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302022-12-0136147248010.1080/13102818.2022.2098056Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.)Ying Wang0Gui Wang1Zhenjie Zhou2Yuan Zong3Lihui Wang4Shipeng Yang5Yaqi Zhang6Xuemei Sun7Qinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR ChinaQinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR ChinaQinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, PR ChinaQinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR ChinaQinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR ChinaQinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR ChinaQinghai Key Laboratory of Genetics and Physiology of Vegetables, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, Qinghai, PR ChinaAbstractJerusalem artichoke (Helianthus tuberosus L.) is a fructan-accumulating plant and an industrial raw material for fructan production. However, the genetic mechanism of fructan biosynthesis remains unclear. Therefore, this study performed transcriptome analysis to determine the genetic differences between two Jerusalem artichoke genotypes and different fructan contents. Approximately 19.73 Gb of clean data were obtained after filtering, and a total of 164,006 unigenes were annotated based on GO, KOG and KEGG functional classification. Then, seven homologous genes involved in fructan biosynthesis were obtained by homology comparison. CL11458.Contig2 and CL7122.Contig14 were candidate genes for sucrose:sucrose 1-fructosyl-transferase (1-SST) and fructan:fructan 1-fructosyl-transferase (1-FFT), respectively. The genetic relationship between SST and SST from artichoke in the phylogenetic tree are relatively close, and FFT is located in the same branch as FFT from ‘Qingyu No.1’ Jerusalem artichoke and Viguiera. SST and FFT were the main regulatory genes of fructan accumulation in Jerusalem artichoke. Therefore, this study successfully identified major genes involved in fructan biosynthesis. Future studies should aim to prove the role of each candidate gene in fructan biosynthesis in Jerusalem artichoke.https://www.tandfonline.com/doi/10.1080/13102818.2022.2098056Helianthus tuberosus L.transcriptome analysissucrose:sucrose 1-fructosyl-transferasefructan:fructan 1-fructosyl-transferase genefructan biosynthesis |
| spellingShingle | Ying Wang Gui Wang Zhenjie Zhou Yuan Zong Lihui Wang Shipeng Yang Yaqi Zhang Xuemei Sun Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.) Biotechnology & Biotechnological Equipment Helianthus tuberosus L. transcriptome analysis sucrose:sucrose 1-fructosyl-transferase fructan:fructan 1-fructosyl-transferase gene fructan biosynthesis |
| title | Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.) |
| title_full | Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.) |
| title_fullStr | Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.) |
| title_full_unstemmed | Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.) |
| title_short | Transcriptome analysis for genes involved in fructan biosynthesis in the Jerusalem artichoke (Helianthus tuberosus L.) |
| title_sort | transcriptome analysis for genes involved in fructan biosynthesis in the jerusalem artichoke helianthus tuberosus l |
| topic | Helianthus tuberosus L. transcriptome analysis sucrose:sucrose 1-fructosyl-transferase fructan:fructan 1-fructosyl-transferase gene fructan biosynthesis |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2022.2098056 |
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